MURPHY 301 EXAM INTRODUCTION TO
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Quiz: The side chain of histidine has a typical pKa value in the range of 6.5-
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7.4. However, when analyzing the pKa values in a particular protein,
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scientists determined that one particular His residue has an unusually low
pKa value of 4.8. Which of the following statements correctly explain this
anomaly?
1) The microenvironment around a residue can impact its pKa value.
2) A positively charged amino acid must be in close proximity to this
residue.
3) A negatively charged amino acid must be in close proximity to this
residue.
4) This residue must be located on the surface of the protein.⼀Answer:
Statement 1 and 2
Quiz: The side chain of ________ has a pKa in the physiological pH
range and is therefore often involved in proton transfer during enzymatic
catalysis.⼀Answer: Histidine
Quiz: All amino acids have a chiral α-carbon EXCEPT ________.⼀
Answer: glycine
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Quiz: Which of the following statements regarding Anfinsen's denaturing
experiments with ribonuclease A are valid?
1) Exposing the denatured protein to air oxidation and then dialysis to
remove urea restored the protein to its original functionality.
2) Removing urea by dialysis and then allowing air oxidation of the
denatured protein restored the protein to its original functionality.
3) Denaturing the protein with both urea and β-mercaptoethanol yielded
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an inactive protein.
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4) Protein folding is determined by its primary sequence.⼀Answer: Only
statements 2, 3, and 4 are valid.
Quiz: Which of the following statements about protein quaternary
structure are correct?
1) It involves a complex of two or more proteins interacting with each other.
2) The subunits of the structure can be either identical or different.
3) The interactions between subunits can give rise to indefinite growth of
polymeric complexes.
4) Most assemblies of protein subunits have one or more defined axis of
rotation.⼀Answer: All statements are correct.
Quiz: In general, enzymes are what kinds of molecules?
proteins
minerals
carbohydrates
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lipids
nucleic acids⼀Answer: proteins
Quiz: The basic building blocks of proteins are _______⼀Answer:
amino acids
Quiz: All amino acids are composed what three things attached to it?⼀
Answer: central α-carbon that has an amine group
a carboxylic acid group
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and an R group
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Quiz: All amino acids except _______ are chiral with the two isomer
forms known as the L-isomer and the D-isomer.⼀Answer: glycine
Quiz: The ____ is the only isomer known to be incorporated into
proteins.⼀Answer: L-isomer
Quiz: This ionic state, where the carboxylic acid is negative and the
amine group is positive, is known as the __________ state⼀Answer:
zwitterionic state
Quiz: What are the N-terminal and C-terminal residues for the following
peptide sequence?
Peptide sequence: EASY⼀Answer: E is the N-terminal residue; Y is the C-
terminal residue.
Quiz: Ultimate source of energy for life on earth is:
A. Sun
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B. Geothermal heat
C. Carbohydrates
D. Fats
E. Other organisms⼀Answer: Sun
Quiz: Which is not a common component of biomolecules?
A. Carbon
B. Hydrogen
C. Oxygen
D. Nitrogen
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E. Chlorine⼀Answer: Chlorine
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Quiz: Which is a basic structural unit of living organisms?
A. Nucleus
B. Cell membrane
C. Tissue
D. Cell
E. Cytoskeleton⼀Answer: Cell
Quiz: Which of the following is not an organelle?
A. Mitochondria
B. Chloroplast
C. Nucleus
D. Plasma Membrane
E. Golgi Complex⼀Answer: Plasma Membrane
Quiz: Which of the following is not a role played by carbohydrates in
living cells?