DPIP Lab
What is the purpose of DPIP in this experiment? – answer DPIP is the electron acceptor
in this experiment.
What molecule found in chloroplasts does DPIP "replace" in this experiment? – answer
DPIP substitutes for the NADP molecules.
What is the source of the electrons that will reduce DPIP? - answer The electrons come
from the photolysis of water.
What was measured with the spectrophotometer in this experiment? - answer The
spectrophotometer measures the percentage of light transmittance through the cuvette
due to DPIP reduction.
What is the effect of darkness on the reduction of DPIP? Explain. - answer The effect of
darkness is that no reaction will occur.
What is the effect of boiling the chloroplasts on the subsequent reduction of DPIP?
Explain. - answerBoiling denatures the protein molecules and stops the reduction.
What reasons can you give for the difference in the percent transmittance between the
live chloroplasts that were incubated in the light and those that were kept in the dark? -
answerIn the dark cuvette, there was no light energy available, so there was no flow of
electrons and no photolysis of water, while in the lighted cuvette these processes were
allowed to continue.
What is the purpose of DPIP in this experiment? – answer DPIP is the electron acceptor
in this experiment.
What molecule found in chloroplasts does DPIP "replace" in this experiment? – answer
DPIP substitutes for the NADP molecules.
What is the source of the electrons that will reduce DPIP? - answer The electrons come
from the photolysis of water.
What was measured with the spectrophotometer in this experiment? - answer The
spectrophotometer measures the percentage of light transmittance through the cuvette
due to DPIP reduction.
What is the effect of darkness on the reduction of DPIP? Explain. - answer The effect of
darkness is that no reaction will occur.
What is the effect of boiling the chloroplasts on the subsequent reduction of DPIP?
Explain. - answerBoiling denatures the protein molecules and stops the reduction.
What reasons can you give for the difference in the percent transmittance between the
live chloroplasts that were incubated in the light and those that were kept in the dark? -
answerIn the dark cuvette, there was no light energy available, so there was no flow of
electrons and no photolysis of water, while in the lighted cuvette these processes were
allowed to continue.
