CLINICAL MICROBIOLOGY QUESTIONS
WITH ANSWERS
Microbiology ✅✅The study of microscopic organisms
BSL 1 ✅✅Microorganisms not known to cause disease in healthy adults and it has no
risk
Bacillus subtilis ✅✅Example of BSL 1
BSL 2 ✅✅Indicates most common microorganisms and it has a moderate risk
Staphylococcus, Hepatitis B virus, HIV, salmonella, shigella ✅✅Example of BSL 2
BSL 3 ✅✅biosafety cabinets to prevent airborne transmission
Mycobacterium tuberculosis, brucella, coccidiodes, immits, rickettsiaea, arboviruses
✅✅Example of BSL 3
BSL 4 ✅✅It is airborne and used in research facilities includes a limited of exotoxic
viruses filovirus & arenavirus
Sterilization, disinfection ✅✅2 chemical germicide categories
Moist heat, dry heat, filtration ✅✅3 types of sterilization
Sterilization ✅✅Removal of all living forma, including their spores
Autoclave, tyndallization, pasteurization, boiling ✅✅4 types of moist heat
121 degrees C and 15 psi (30 minutes) ✅✅What is the temperature and time of
autoclave?
100 degrees C (30mins for 3 days) ✅✅What is the temperature and time of
tyndallization?
63 degrees C (30mins); 72 degrees C (15secs) ✅✅What is the temperature and time
of pasteurization?
100 degrees C (15-30mins) ✅✅What is the temperature and time of boiling?
Oven, incineration, flaming, cremation ✅✅4 types of dry heat
WITH ANSWERS
Microbiology ✅✅The study of microscopic organisms
BSL 1 ✅✅Microorganisms not known to cause disease in healthy adults and it has no
risk
Bacillus subtilis ✅✅Example of BSL 1
BSL 2 ✅✅Indicates most common microorganisms and it has a moderate risk
Staphylococcus, Hepatitis B virus, HIV, salmonella, shigella ✅✅Example of BSL 2
BSL 3 ✅✅biosafety cabinets to prevent airborne transmission
Mycobacterium tuberculosis, brucella, coccidiodes, immits, rickettsiaea, arboviruses
✅✅Example of BSL 3
BSL 4 ✅✅It is airborne and used in research facilities includes a limited of exotoxic
viruses filovirus & arenavirus
Sterilization, disinfection ✅✅2 chemical germicide categories
Moist heat, dry heat, filtration ✅✅3 types of sterilization
Sterilization ✅✅Removal of all living forma, including their spores
Autoclave, tyndallization, pasteurization, boiling ✅✅4 types of moist heat
121 degrees C and 15 psi (30 minutes) ✅✅What is the temperature and time of
autoclave?
100 degrees C (30mins for 3 days) ✅✅What is the temperature and time of
tyndallization?
63 degrees C (30mins); 72 degrees C (15secs) ✅✅What is the temperature and time
of pasteurization?
100 degrees C (15-30mins) ✅✅What is the temperature and time of boiling?
Oven, incineration, flaming, cremation ✅✅4 types of dry heat
