, TEST BANK for Fundamentals of MolecularVirology 2nd
t t t t t t t
Edition by Acheson. t t t
Package Title: Testbank
t t
Course Title: Acheson 2nd edition
t t t t
Chapter Number: 01
t t t
Question type: Multiple Choice
t t t
1) Which of the following terms describes the protein shell that surrounds the viral genome?
t t t t t t t t t t t t t
a) capsid
b) envelope
c) matrix
d) virion
e) capsomere
Answer: a
t t
2) Which of the following would not be a nucleic acid form found in a viral genome?
t t t t t t t t t t t t t t t
a) dsDNA
b) ssDNA
c) dsRNA
d) ssRNA
e) an RNA:DNA hybrid
t t
Answer: e
t t
3) Which of the following is an enzyme that most RNA viruses encode in their genome?
t t t t t t t t t t t t t t
a) DNA-dependent-RNA-polymerase
b) RNA-dependent-RNA-polymerase
c) DNA-dependent-DNA-polymerase
d) DNA-dependent-RNA-polymerase
e) RNA-dependent-protein-synthetase
Answer: b
t t
4) Viruses play a major role in the ecology of the ocean by doing which of the following?
t t t t t t t t t t t t t t t t
a) By infecting photosynthetic bacteria and increasing their growth.
t t t t t t t
,b) By infecting harmful bacteria found in the ocean and limiting their growth.
t t t t t t t t t t t
c) By killing off fish and preventing them from becoming too plentiful.
t t t t t t t t t t
d) By lysing unicellular organisms and releasing carbon and oxygen back into the ocean.
t t t t t t t t t t t t
e) All of the above statements are correct.
t t t t t t
Answer: d t
5) If you wanted to show that an infectious agent is a virus, what experiment could you do?
t t t t t t t t t t t t t t t t
a) Show that the agent can be seen under a light microscope.
t t t t t t t t t t
b) Show that agent contains a lipid membrane.
t t t t t t
c) Show that the agent can form single colonies on an agar plate.
t t t t t t t t t t t
d) Show that the agent can be diluted and can still cause disease in an animal.
t t t t t t t t t t t t t t
e) Show that the agent can pass through a porcelain filter and is still infectious.
t t t t t t t t t t t t t
Answer: e t
6) What does the word “virus” mean in Latin?
t t t t t t t
a) infectious agent t
b) liquid
c) poison
d) death
e) disease
Answer: c
t t
7) The word “phage” is a shortened version of the name of a virus that can infect which type of
t t t t t t t t t t t t t t t t t t
organism?
a) bacterial cell t
b) human cell t
c) eukaryotic cell t
d) plant cell t
e) inset cell t
Answer: a
t t
8) The study of which group of viruses led to the discovery of cellular genes that can promoter cancer in
t t t t t t t t t t t t t t t t t t
animal cells?
t t
a) papillomaviruses
b) retroviruses
c) adneoviruses
d) polyomaviruses
, e) picornaviruses
Answer: b
t t
9) When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is
t t t t t t t t t t t t t t t t t t
put on top of the cells after they have been infected with the diluted virus. What is the major purpose of
t t t t t t t t t t t t t t t t t t t t t
this agar layer?
t t t
a) It helps to feed the cells as they grow.
t t t t t t t t
b) It promotes the replication of the virus.
t t t t t t
c) It makes the infected cells easier to visualize.
t t t t t t t
d) It limits the movement of virus particles.
t t t t t t
e) All of the above are correct.
t t t t t
Answer: d t
10) To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x
t t t t t t t t t t t t t t t t t t t t t t
107 pfu/ml onto a plate of animal cells that contains 5 x 106 cells. What is the multiplicity of infection for
t t t t t t t t t t t t t t t t t t t t t
this experiment?
t t
a) 12
b) 6
c) 5
d) 3
e) 1
Answer: b
t t
11) During a single growth cycle experiment, the titer of extracellular and intracellular virus drops in the
t t t t t t t t t t t t t t t
first hour. What explains this observation?
t t t t t t
a) The virus is inactivated by antibodies in the medium.
t t t t t t t t
b) The virus is inactivated by cellular enzymes.
t t t t t t
c) The virus enters the cell and is uncoated.
t t t t t t t
d) The virus binds to the cells in the dish and can’t be released.
t t t t t t t t t t t t
e) The cell’s antiviral defenses prevent replication of the virus
t t t t t t t t
Answer: c t
12) What is another term for an RNA-dependent-DNA-polymerase?
t t t t t t
a) DNA polymerase t
b) RNA polymerase II t t
t t t t t t t
Edition by Acheson. t t t
Package Title: Testbank
t t
Course Title: Acheson 2nd edition
t t t t
Chapter Number: 01
t t t
Question type: Multiple Choice
t t t
1) Which of the following terms describes the protein shell that surrounds the viral genome?
t t t t t t t t t t t t t
a) capsid
b) envelope
c) matrix
d) virion
e) capsomere
Answer: a
t t
2) Which of the following would not be a nucleic acid form found in a viral genome?
t t t t t t t t t t t t t t t
a) dsDNA
b) ssDNA
c) dsRNA
d) ssRNA
e) an RNA:DNA hybrid
t t
Answer: e
t t
3) Which of the following is an enzyme that most RNA viruses encode in their genome?
t t t t t t t t t t t t t t
a) DNA-dependent-RNA-polymerase
b) RNA-dependent-RNA-polymerase
c) DNA-dependent-DNA-polymerase
d) DNA-dependent-RNA-polymerase
e) RNA-dependent-protein-synthetase
Answer: b
t t
4) Viruses play a major role in the ecology of the ocean by doing which of the following?
t t t t t t t t t t t t t t t t
a) By infecting photosynthetic bacteria and increasing their growth.
t t t t t t t
,b) By infecting harmful bacteria found in the ocean and limiting their growth.
t t t t t t t t t t t
c) By killing off fish and preventing them from becoming too plentiful.
t t t t t t t t t t
d) By lysing unicellular organisms and releasing carbon and oxygen back into the ocean.
t t t t t t t t t t t t
e) All of the above statements are correct.
t t t t t t
Answer: d t
5) If you wanted to show that an infectious agent is a virus, what experiment could you do?
t t t t t t t t t t t t t t t t
a) Show that the agent can be seen under a light microscope.
t t t t t t t t t t
b) Show that agent contains a lipid membrane.
t t t t t t
c) Show that the agent can form single colonies on an agar plate.
t t t t t t t t t t t
d) Show that the agent can be diluted and can still cause disease in an animal.
t t t t t t t t t t t t t t
e) Show that the agent can pass through a porcelain filter and is still infectious.
t t t t t t t t t t t t t
Answer: e t
6) What does the word “virus” mean in Latin?
t t t t t t t
a) infectious agent t
b) liquid
c) poison
d) death
e) disease
Answer: c
t t
7) The word “phage” is a shortened version of the name of a virus that can infect which type of
t t t t t t t t t t t t t t t t t t
organism?
a) bacterial cell t
b) human cell t
c) eukaryotic cell t
d) plant cell t
e) inset cell t
Answer: a
t t
8) The study of which group of viruses led to the discovery of cellular genes that can promoter cancer in
t t t t t t t t t t t t t t t t t t
animal cells?
t t
a) papillomaviruses
b) retroviruses
c) adneoviruses
d) polyomaviruses
, e) picornaviruses
Answer: b
t t
9) When doing a plaque assay with human or animal cells, a layer of nutrient media mixed with agar is
t t t t t t t t t t t t t t t t t t
put on top of the cells after they have been infected with the diluted virus. What is the major purpose of
t t t t t t t t t t t t t t t t t t t t t
this agar layer?
t t t
a) It helps to feed the cells as they grow.
t t t t t t t t
b) It promotes the replication of the virus.
t t t t t t
c) It makes the infected cells easier to visualize.
t t t t t t t
d) It limits the movement of virus particles.
t t t t t t
e) All of the above are correct.
t t t t t
Answer: d t
10) To start an experiment, you do an infection by putting 0.5 ml of a virus stock that has a titer of 6 x
t t t t t t t t t t t t t t t t t t t t t t
107 pfu/ml onto a plate of animal cells that contains 5 x 106 cells. What is the multiplicity of infection for
t t t t t t t t t t t t t t t t t t t t t
this experiment?
t t
a) 12
b) 6
c) 5
d) 3
e) 1
Answer: b
t t
11) During a single growth cycle experiment, the titer of extracellular and intracellular virus drops in the
t t t t t t t t t t t t t t t
first hour. What explains this observation?
t t t t t t
a) The virus is inactivated by antibodies in the medium.
t t t t t t t t
b) The virus is inactivated by cellular enzymes.
t t t t t t
c) The virus enters the cell and is uncoated.
t t t t t t t
d) The virus binds to the cells in the dish and can’t be released.
t t t t t t t t t t t t
e) The cell’s antiviral defenses prevent replication of the virus
t t t t t t t t
Answer: c t
12) What is another term for an RNA-dependent-DNA-polymerase?
t t t t t t
a) DNA polymerase t
b) RNA polymerase II t t
