BIOL 3001 QUIZ QUESTIONS WITH
CORRECT ANSWERS!!
____________ is a series of techniques by which biomolecules can be separated
based on size, length, and/or shape - ANSWERelectrophoresis
You will be using a new computer program this week called ELS which stands for
Electrophoresis Learning System - ANSWERTrue
The scientific name (genus species) for the fruit fly (red-eyed pomace fly) is? -
ANSWERDrosophila melanogaster
How many PAIRS of chromosomes doe fruit flies have? - ANSWER4
For the Lygaeus Mode of Sex-determination, list the chromosome assignment for
each gender - ANSWERFemale: XX
Male: XY
Why are the Parentals of most crosses homozygous (1 dominant and 1 recessive)? -
ANSWERall offspring in first Filial (F1) will be heterozygous
A total of 428 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many red offspring
did you expect from the 428 progeny? - ANSWER321
When using the ELS program, the samples should be heated for a minimum of
__________ minutes - ANSWER5
The fragments of DNA we will be examining this week in lab will be separated based
on fragment _________ - ANSWERlength
why it is so important to control the time of gel electrophoresis. what might happen if
the gel runs too short of a time versus too long of a time. - ANSWER1. not enough
resolution/separation of the band fragments if the gel runs too short making it difficult
to interpret and 2. loss of data if bands are run off the end of the gel
The final step in the ELS program is to develop your gel so that the DNA fragments
become visible. This technique uses a small amount of radioactive phosphorus and
is called ___________ - ANSWERautoradiography
The DNA fragments used in this lab will exhibit a very simple mode of inheritance,
which is ___________ - ANSWERco-dominance
When using microsatellites as genetic markers, as we do in this lab exercise, it is
easy to identify heterozygotes because they all show 2 bands. True or False? -
ANSWERTrue
, Genotyping also provides us with another critical lesson, namely that __________ -
ANSWERuncertainly exists in all scientific studies
A total of 3852 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring
did you expect from the 3852 progeny? - ANSWER963
A total of 424 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring
did you expect from the 424 progeny? - ANSWER106
the National Institute of Health created a free repository called __________ -
ANSWERGeneStore
To determine the identity of your DNA samples, we will be using a search program
provided by the NIH (National Institute of Health). This program is called
__________. - ANSWERBLAST
Mendel's Second Principle describes the interaction of 2 or more loci and is call the
Principle of Independent ______________. - ANSWERAssortment
In a recent survey of students in General Genetics, 102 identified themselves are
female and 171 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
____? - ANSWER17.44
In a recent survey of students in General Genetics, 97 identified themselves are
female and 114 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
____? - ANSWER1.37
In a recent survey of students in General Genetics, 511 identified themselves are
female and 396 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
_________? - ANSWER14.58
__________ is a technique for determining the precise order of the nucleotides in a
DNA fragment. - ANSWERDNA sequencing
PCR is a laboratory technique that allows geneticists to precisely generate millions of
copies of small fragments of DNA. The letters PCR are an abbreviation for
________________. - ANSWERPolymerase Chain Reaction
The most commonly used sequencing technique was developed in 1977 by Dr. Fred
Sanger and is called ___________. - ANSWERChain Termination sequencing
5 main components of the Sanger sequencing technique: - ANSWER1. DNA
template
2. DNA primer
CORRECT ANSWERS!!
____________ is a series of techniques by which biomolecules can be separated
based on size, length, and/or shape - ANSWERelectrophoresis
You will be using a new computer program this week called ELS which stands for
Electrophoresis Learning System - ANSWERTrue
The scientific name (genus species) for the fruit fly (red-eyed pomace fly) is? -
ANSWERDrosophila melanogaster
How many PAIRS of chromosomes doe fruit flies have? - ANSWER4
For the Lygaeus Mode of Sex-determination, list the chromosome assignment for
each gender - ANSWERFemale: XX
Male: XY
Why are the Parentals of most crosses homozygous (1 dominant and 1 recessive)? -
ANSWERall offspring in first Filial (F1) will be heterozygous
A total of 428 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many red offspring
did you expect from the 428 progeny? - ANSWER321
When using the ELS program, the samples should be heated for a minimum of
__________ minutes - ANSWER5
The fragments of DNA we will be examining this week in lab will be separated based
on fragment _________ - ANSWERlength
why it is so important to control the time of gel electrophoresis. what might happen if
the gel runs too short of a time versus too long of a time. - ANSWER1. not enough
resolution/separation of the band fragments if the gel runs too short making it difficult
to interpret and 2. loss of data if bands are run off the end of the gel
The final step in the ELS program is to develop your gel so that the DNA fragments
become visible. This technique uses a small amount of radioactive phosphorus and
is called ___________ - ANSWERautoradiography
The DNA fragments used in this lab will exhibit a very simple mode of inheritance,
which is ___________ - ANSWERco-dominance
When using microsatellites as genetic markers, as we do in this lab exercise, it is
easy to identify heterozygotes because they all show 2 bands. True or False? -
ANSWERTrue
, Genotyping also provides us with another critical lesson, namely that __________ -
ANSWERuncertainly exists in all scientific studies
A total of 3852 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring
did you expect from the 3852 progeny? - ANSWER963
A total of 424 offspring are produced. Some are red and some are blue. Given the
cross that you made, you expected a ratio of 3 red : 1 blue. How many blue offspring
did you expect from the 424 progeny? - ANSWER106
the National Institute of Health created a free repository called __________ -
ANSWERGeneStore
To determine the identity of your DNA samples, we will be using a search program
provided by the NIH (National Institute of Health). This program is called
__________. - ANSWERBLAST
Mendel's Second Principle describes the interaction of 2 or more loci and is call the
Principle of Independent ______________. - ANSWERAssortment
In a recent survey of students in General Genetics, 102 identified themselves are
female and 171 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
____? - ANSWER17.44
In a recent survey of students in General Genetics, 97 identified themselves are
female and 114 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
____? - ANSWER1.37
In a recent survey of students in General Genetics, 511 identified themselves are
female and 396 as male. Use Chi-Squared analysis to determine if this sample fits
an expected ratio of 1:1. The Observed Chi-Squared calculated from these data is
_________? - ANSWER14.58
__________ is a technique for determining the precise order of the nucleotides in a
DNA fragment. - ANSWERDNA sequencing
PCR is a laboratory technique that allows geneticists to precisely generate millions of
copies of small fragments of DNA. The letters PCR are an abbreviation for
________________. - ANSWERPolymerase Chain Reaction
The most commonly used sequencing technique was developed in 1977 by Dr. Fred
Sanger and is called ___________. - ANSWERChain Termination sequencing
5 main components of the Sanger sequencing technique: - ANSWER1. DNA
template
2. DNA primer
