UIUC MCB 150 EXAM 2 STUDY GUIDE
Topoisomerase - Answers :Wind, unwind, cut, and supercoil DNA
Nuclease - Answers :Enzyme that degrades DNA
Nucleosomes - Answers :8 proteins, 147 base pairs
Histones - Answers :Small, basic proteins
-H1, H2A, H2B, H3, H4
Chromatosome - Answers :Nucleosome, 10 bp DNA on each side, H1
Leading Strand - Answers :Strand that can go through DNA synthesis uninterrupted
Lagging Strand - Answers :Starts away from ori, then works backwards
Okazaki Fragments - Answers :Small fragments of DNA in lagging strand
Euchromatin - Answers :Loosely condensed, found throughout nucleus in 30-nm fibers
Heterochromatin - Answers :Highly condensed, found in periphery of nucleus
Helicase - Answers :Enzyme that separates DNA strands
Primase - Answers :DNA-dependent, RNA-synthesizing enzyme
DNA Polymerase III
(E. Coli) - Answers :Synthesizes the new DNA molecule
DNA Polymerase I - Answers :Removes primers and replaces w/ DNA nucleotides
DNA Ligase - Answers :ATP-dependent, seals nicks in strand
Transcription - Answers :Making RNA from DNA
RNA Polymerase
(E. Coli) - Answers :Copies DNA into RNA
Holoenzyme - Answers :6 units of the Polymerase together
Core Enzyme - Answers :Polymerase without pi subunit
Promoters - Answers :Markers for beginning of genes
Coding strand - Answers :Non-template strand
Topoisomerase - Answers :Wind, unwind, cut, and supercoil DNA
Nuclease - Answers :Enzyme that degrades DNA
Nucleosomes - Answers :8 proteins, 147 base pairs
Histones - Answers :Small, basic proteins
-H1, H2A, H2B, H3, H4
Chromatosome - Answers :Nucleosome, 10 bp DNA on each side, H1
Leading Strand - Answers :Strand that can go through DNA synthesis uninterrupted
Lagging Strand - Answers :Starts away from ori, then works backwards
Okazaki Fragments - Answers :Small fragments of DNA in lagging strand
Euchromatin - Answers :Loosely condensed, found throughout nucleus in 30-nm fibers
Heterochromatin - Answers :Highly condensed, found in periphery of nucleus
Helicase - Answers :Enzyme that separates DNA strands
Primase - Answers :DNA-dependent, RNA-synthesizing enzyme
DNA Polymerase III
(E. Coli) - Answers :Synthesizes the new DNA molecule
DNA Polymerase I - Answers :Removes primers and replaces w/ DNA nucleotides
DNA Ligase - Answers :ATP-dependent, seals nicks in strand
Transcription - Answers :Making RNA from DNA
RNA Polymerase
(E. Coli) - Answers :Copies DNA into RNA
Holoenzyme - Answers :6 units of the Polymerase together
Core Enzyme - Answers :Polymerase without pi subunit
Promoters - Answers :Markers for beginning of genes
Coding strand - Answers :Non-template strand
