MLS Program Comprehensive Exam Questions with Correct Answers Graded A+
What antibody is most associated with delayed hemolytic transfusion reactions? - Answers Anti-Jka
What antibody is associated with Mycoplasma pneumonia? - Answers Anti-I
What class of antibodies cause extravascular hemolysis from sequestration by spleen? - Answers IgG
Enzyme panel will not react with which antibodes? - Answers Duffy (Fya or Fyb)
What can cause a mother to have a low titer anti-D? - Answers RhIg at 28 weeks
(T/F) Blood from mom to child must be irradiated. - Answers True
What kind of errors can room temp cross match detect? - Answers ABO
What is the main reason premature infants need transfusions? - Answers Blood loss from lab tests (aka
iatrogenic blood loss)
What type of positive control should be used when phenotyping? - Answers Heterozyous
What could make the auto control positive? - Answers Rouleaux or cold agglutinin
How would you correct rouleaux? - Answers Saline replacement
How would you fix cold agglutinin results? - Answers Prewarm
Cryoprecipitate is used today mostly to replace what? - Answers Fibrinogen
What type of plasma can an AB = patient receive? - Answers only AB
What are the requirements for intrauterine transfusions? - Answers Fresh, O neg, CMV neg, irradiated,
collected with CPDA
When is FFP used? - Answers When PT and PTT are prolonged
What temperature do Lewis antibodies react? - Answers RT and sometimes 37
What affect can Lewis antibodies have on samples? - Answers Can cause in vitro hemolysis
How long would a sexual partner of an IV drug user be deferred from donating? - Answers One year
What can make the Rh control positive when doing a weak D test? - Answers Positive DAT
What medication does PT monitor? - Answers Coumadin (Warfarin)
Protein C deficiency causes what? - Answers Thrombosis
What factor deficiency causes thrombosis instead of bleeding? - Answers XII
, What parameter does a cold agglutinin NOT affect? - Answers Hemoglobin
What will the cells of an aged sample look like? - Answers crenated red cells and vacuoles in white cells
What two RBC morphologies will lower a sed rate? - Answers Sickle cells and spherocytes
What changes should be made to a PT or PTT sample collected on a patient with polcythemia? - Answers
Anticoagulant should be adjusted to correct for less plasma
Megaloblastic anemia is associated with what? - Answers Hypersegmented neutrophils, pancytopenia,
and macrocytes
What are Dohle bodies? - Answers Blue oblong inclusions located near the periphery of seg
What are Dohle bodies composed of and when are they seen? - Answers RNA or rough endoplasmic
reticulum; Bacterial infection, inflammation, or burns
What are Howell-Jolly bodies composed of and when are they seen? - Answers DNA; megaloblastic
anemia, splenomegaly or splenectomy, thalassemia, sickle cell, or hemolytic anemia
What is basophilic stippling and what does it indicate/when is it seen? - Answers Denatured RNA;
altered or impaired hemoglobin synthesis/lead poisoning, thalassemia
What are Pappenheimer bodies called when Prussian blue stain is used? - Answers Siderocytes
What are Pappenheimer bodies composed of and when are they seen? - Answers Iron; sideroblastic
anemia (iron overload), thalassemia, splenectomy
What stain is used for Heinz bodies? - Answers Supravital (crystal violet or new methylene blue)
What are Heinz bodies composed of and when are they seen? - Answers Denatured or precipitated
hemoglobin; G6Pd deficiency, unstable hemoglobin causing iron to be unprotected, oxidizing drugs,
alpha thalassemia
What are cabot rings composed of and when are they seen? - Answers Remnants of spindle fibers;
severe anemias, pernicious anemia, dyserythropoiesis
Describe hemoglobin C crystals - Answers Oblong dark crystal in Hemoglobin C disease
What hgb electrophoresis results are seen in hgb C disease? - Answers 90% of the hemoglobin is hgb C
and the rest is A2 and F
Lab results in multiple myeloma - Answers Monoclonal M spike on protein electrophoresis, Bence Jones
proteins in urine, norm/norm anemia, pancytopenia in advanced stages, rouleaux, "sheets" of plasma
cells in bone marrow (usually 30% plasma cells)
How is osmotic fragility affected in hereditary spherocytosis? - Answers Increased
What antibody is most associated with delayed hemolytic transfusion reactions? - Answers Anti-Jka
What antibody is associated with Mycoplasma pneumonia? - Answers Anti-I
What class of antibodies cause extravascular hemolysis from sequestration by spleen? - Answers IgG
Enzyme panel will not react with which antibodes? - Answers Duffy (Fya or Fyb)
What can cause a mother to have a low titer anti-D? - Answers RhIg at 28 weeks
(T/F) Blood from mom to child must be irradiated. - Answers True
What kind of errors can room temp cross match detect? - Answers ABO
What is the main reason premature infants need transfusions? - Answers Blood loss from lab tests (aka
iatrogenic blood loss)
What type of positive control should be used when phenotyping? - Answers Heterozyous
What could make the auto control positive? - Answers Rouleaux or cold agglutinin
How would you correct rouleaux? - Answers Saline replacement
How would you fix cold agglutinin results? - Answers Prewarm
Cryoprecipitate is used today mostly to replace what? - Answers Fibrinogen
What type of plasma can an AB = patient receive? - Answers only AB
What are the requirements for intrauterine transfusions? - Answers Fresh, O neg, CMV neg, irradiated,
collected with CPDA
When is FFP used? - Answers When PT and PTT are prolonged
What temperature do Lewis antibodies react? - Answers RT and sometimes 37
What affect can Lewis antibodies have on samples? - Answers Can cause in vitro hemolysis
How long would a sexual partner of an IV drug user be deferred from donating? - Answers One year
What can make the Rh control positive when doing a weak D test? - Answers Positive DAT
What medication does PT monitor? - Answers Coumadin (Warfarin)
Protein C deficiency causes what? - Answers Thrombosis
What factor deficiency causes thrombosis instead of bleeding? - Answers XII
, What parameter does a cold agglutinin NOT affect? - Answers Hemoglobin
What will the cells of an aged sample look like? - Answers crenated red cells and vacuoles in white cells
What two RBC morphologies will lower a sed rate? - Answers Sickle cells and spherocytes
What changes should be made to a PT or PTT sample collected on a patient with polcythemia? - Answers
Anticoagulant should be adjusted to correct for less plasma
Megaloblastic anemia is associated with what? - Answers Hypersegmented neutrophils, pancytopenia,
and macrocytes
What are Dohle bodies? - Answers Blue oblong inclusions located near the periphery of seg
What are Dohle bodies composed of and when are they seen? - Answers RNA or rough endoplasmic
reticulum; Bacterial infection, inflammation, or burns
What are Howell-Jolly bodies composed of and when are they seen? - Answers DNA; megaloblastic
anemia, splenomegaly or splenectomy, thalassemia, sickle cell, or hemolytic anemia
What is basophilic stippling and what does it indicate/when is it seen? - Answers Denatured RNA;
altered or impaired hemoglobin synthesis/lead poisoning, thalassemia
What are Pappenheimer bodies called when Prussian blue stain is used? - Answers Siderocytes
What are Pappenheimer bodies composed of and when are they seen? - Answers Iron; sideroblastic
anemia (iron overload), thalassemia, splenectomy
What stain is used for Heinz bodies? - Answers Supravital (crystal violet or new methylene blue)
What are Heinz bodies composed of and when are they seen? - Answers Denatured or precipitated
hemoglobin; G6Pd deficiency, unstable hemoglobin causing iron to be unprotected, oxidizing drugs,
alpha thalassemia
What are cabot rings composed of and when are they seen? - Answers Remnants of spindle fibers;
severe anemias, pernicious anemia, dyserythropoiesis
Describe hemoglobin C crystals - Answers Oblong dark crystal in Hemoglobin C disease
What hgb electrophoresis results are seen in hgb C disease? - Answers 90% of the hemoglobin is hgb C
and the rest is A2 and F
Lab results in multiple myeloma - Answers Monoclonal M spike on protein electrophoresis, Bence Jones
proteins in urine, norm/norm anemia, pancytopenia in advanced stages, rouleaux, "sheets" of plasma
cells in bone marrow (usually 30% plasma cells)
How is osmotic fragility affected in hereditary spherocytosis? - Answers Increased
