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VERIFIED QUESTIONS AND CORRECT ANSWERS
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1. Aminoacyl-trna isynthetases link to itheir irespective
itrna i molecules
a) Acetylation iof ithe iN iterminal idomain iof ia ilarge iRNA ipolymerase
iII i subunit
b) Phosphorylation iof ithe iC iterminal idomain iof ia ilarge iRNA
ipolymerase i subunit
c) Phosphorylation iof ithe iN iterminal idomain iof ia ilarge iRNA
ipolymerase i subunit
d) Methylation iof ithe iC iterminal idomain iof ia ilarge iRNA ipolymerase
iII i subunit
e) Methylation iof ithe iN iterminal idomain iof ia ilarge iRNA ipolymerase
iII i subunit
i- iANSWERS-e) iMethylation iof ithe iN iterminal idomain iof ia ilarge
iRNA i polymerase iII isubunit
2. During ithe ielongation istep iof itranslation, ithe iribosome iis ibound ito iappropriate
i tRNAS iin iboth ithe iA iand ithe iP isites iand iis iready ifor ipeptidyl itransfer. iWhat
iwill i happen inext?
A) The iamino iend iof ithe iamino iacid iis ireleased ifrom ithe iA isite
i tRNA, iand ijoined ito ithe icarboxyl igroup iof ithe ipolypeptide
ichain i linked ito ithe ip isite itRNA
B) None iof ithese
C) The icarboxyl iend iof ithe iamino iacid iis ireleased ifrom ithe iA
isite i tRNA iand ijoined ito ifree iamino igroup iof ithe
ipolypeptide ichain i lined ito ithe iP isite itRNA.
D) The iamino iend iof ithe ipolypeptide ichain iis ireleased ifrom ithe iP
isite i tRNA iand ijoined ito ithe ifree icarboxyl igroup iof ithe iamino
iacid i linked ito ithe iA isite itRNA
E) The icarboxyl iend iof ithe ipolypeptide ichain iis ireleased ifrom ithe
iP i site itRNA iand ijoined ito ithe ifree iamino igroup iof ithe iamino
iacid i linked ito ithe iA isite itRNA
i- iANSWERS-A) iThe iamino iend iof ithe iamino iacid iis
ireleased i from ithe iA isite itRNA, iand ijoined ito ithe icarboxyl
igroup iof ithe i polypeptide ichain ilinked ito ithe ip isite itRNA
, 3. Eukaryotic ipre imrna's iundergo ia inumber iof imodifications isuch ias icapping iat
ithe i 5' iend. iA i5' icap...
a) Consists iof ia imodified iterminal iadenine inucleotide
b) Carries ia inegative icharge iin ithe iterminal ibase idue ito imethylation
c) Prevents iinitiation iof itranslation
d) Marks ithe imRNA ifor itranslation
e) Contains ia itriphosphate ibridge ibetween ithe iterminal ibase iand ithe i5'
iend i of ithe ipre-mrna
i- iANSWERS-e) iContains ia itriphosphate ibridge ibetween ithe iterminal
ibase i and ithe i5' iend iof ithe ipre-mrna
4. How imany idifferent irna ipolymerases iare ithere iin ieukaryotes?
A) 3
B) 2
C) 1
D) 4
E) 8
i- iANSWERS-3
5. in iprokaryotes ithe iribosomal ibinding isite ion imrna iis icalled?
i- iANSWERS-The iribosomal ibinding isite i(RBS) ion imRNA iin iprokaryotes iis
icalled i the iShine-Dalgarno isequence. iIt iis ia ishort inucleotide isequence ilocated ia
ifew i nucleotides iupstream iof ithe istart icodon i(usually iAUG)
6. In ithe iMeselson istahl iDNA ireplication iexperiment i, iwhat ipercentage iof iDNA
i was icomposed iof ione ilight istrand iand ione iheavy istrand iafter ione igeneration
iof i growth iin iN-14 icontaining igrowth imedia?
a) 25
b) 50
c) 0
id) i
i 100
e) i i 75
i- iANSWERS-50
7. OriC iis ito ie.coli ias is ito iyeast:
a) Replication ifactories
b) DNA ipolymerase idelta
c) Heterochromatin
d) Autonomously ireplicating isequence
e) Origin irecognition icomplex
i- iANSWERS-e) iOrigin irecognition icomplex