biol 227 Lab Exam #2 Questions with
Complete Answers
Using a microscope
Clean lenses, eyepiece, and objectives with lense paper
turn on light to midlevel
Raise condenser to highest position
Place slide on stage and center
Using 100x objective, raise stage until slide almost touches
Find specimen with 4x
Rotate 10x and bring into focus with fine adjustment
Adjust interpupillary distant and diopter adjustments
Kohler illumination
Purpose of Kohler illumination
Allows for high contrast and resolution with reduced heat on the specimen
Kohler illumination
Contrast level/aperture iris to middle
close field iris diaphragm, center the circle
lower condenser until polygon is clear
open field iris to edge of FOV
bright field
dark specimen on bright background
dark field
bright specimen on dark background (good for transparent specimen)
Oil Immerision
,drop of oil on slide, turn to 100x, focus with fine adjustment
measuring field of view
move stage until lines of ruler align with edge, count (in mm), convert to microm
(1mm=1000microm)
CFU/mL
colony count/total dilution factor
total dilution factor
, dilution factor x volume transferred
serial dilution factor
diluted several times in sequence
colony morphology
the properties and characteristics of colonies such as shape, margin, and the elevation
colony morphology shape
circular, spindle, irregular, filamentous, rhizoids
colony morphology margin
entire, undulate, lobate, curled, rhizoid, filamentous
colony morphology elevation
flat, raised, convex, pulvinate, umbonate
colony morphology size
punctiform, small, moderate, large
colony morphology texture
smooth or rough
colony morphology apperance
shiny or dull
colony morphology pigment
color
colony morphology optical property
opaque, translucent, transparent
cell morphology shapes
Complete Answers
Using a microscope
Clean lenses, eyepiece, and objectives with lense paper
turn on light to midlevel
Raise condenser to highest position
Place slide on stage and center
Using 100x objective, raise stage until slide almost touches
Find specimen with 4x
Rotate 10x and bring into focus with fine adjustment
Adjust interpupillary distant and diopter adjustments
Kohler illumination
Purpose of Kohler illumination
Allows for high contrast and resolution with reduced heat on the specimen
Kohler illumination
Contrast level/aperture iris to middle
close field iris diaphragm, center the circle
lower condenser until polygon is clear
open field iris to edge of FOV
bright field
dark specimen on bright background
dark field
bright specimen on dark background (good for transparent specimen)
Oil Immerision
,drop of oil on slide, turn to 100x, focus with fine adjustment
measuring field of view
move stage until lines of ruler align with edge, count (in mm), convert to microm
(1mm=1000microm)
CFU/mL
colony count/total dilution factor
total dilution factor
, dilution factor x volume transferred
serial dilution factor
diluted several times in sequence
colony morphology
the properties and characteristics of colonies such as shape, margin, and the elevation
colony morphology shape
circular, spindle, irregular, filamentous, rhizoids
colony morphology margin
entire, undulate, lobate, curled, rhizoid, filamentous
colony morphology elevation
flat, raised, convex, pulvinate, umbonate
colony morphology size
punctiform, small, moderate, large
colony morphology texture
smooth or rough
colony morphology apperance
shiny or dull
colony morphology pigment
color
colony morphology optical property
opaque, translucent, transparent
cell morphology shapes
