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PCB 3134 TEST 4, PCB3134 Eukaryotic
Cell Structure Practice Exam 4, PCB
3134 EXAM 4
Chromosome painting involves
a. staining chromosomes with Giemsa reagent.
b. hybridizing fluorescent probes to chromosomes.
c. hybridizing radioactive probes to chromosomes.
d. a and b - ans-b. hybridizing fluorescent probes to chromosomes.
Which of the following occurs during
anaphase A?
a. The spindle elongates.
b. Kinetochores remain attached to shortening
kinetochore microtubules.
c. Chromosomes move to the spindle equator.
d. The spindle poles move closer together. - ans-b. Kinetochores remain attached to shortening
kinetochore microtubules.
During which stage of the cell cycle is the
chromosome content of a mammalian liver cell
1n?
a. G1
b. S
c. G0
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dM
e none of the above - ans-e none of the above
Human genes/proteins that regulate the cell
cycle are most easily isolated by
a. biochemical purification.
b. complementation of Xenopus mutants.
c. complementation of yeast mutants.
d. positional cloning - ans-c. complementation of yeast mutants.
Kinetochores assemble at the
a. centrosome.
b. spindle pole.
c. telomere.
d. centromere. - ans-d. centromere.
Exit from mitosis depends upon the
degradation of
a. cohesion.
b. condensin.
c. mitotic cyclin.
d. securin.
e. separase. - ans-c. mitotic cyclin.
Separase initiates sister chromatid
segregation at anaphase by cleaving
a. APC.
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b. Cdc20.
c. cyclin B.
d. cohesin.
e. securin. - ans-d. cohesin.
Phosphorylation of Thr-161 on S. pombe
MPF
a. is required for MPF activity.
b. inhibits MPF activity.
c. is carried out by Wee1 protein.
d. b and c - ans-a. is required for MPF activity.
Which of the following histone modification
likely leads to chromatin condensation?
a. Phosphoryaltion.
b. Glycosylation.
c. Deacetylation.
d. Ubiquitination. - ans-c. Deacetylation.
All of the following can be found in
chromatin except
a. DNA.
b. histones.
c. RNA.
d. condensin. - ans-c. RNA.
All the following statements about
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heterochromatin are true except
a. It is a dark-staining area of a chromosome.
b. It is usually transcriptionally active.
c. It is often simple sequence DNA.
d. It is a region of condensed chromatin - ans-b. It is usually transcriptionally active.
In the mitotic spindle, kinetochore
microtubules function to
a. connect the spindle poles.
b. attach chromosomes to the spindle.
c. carry out cytokinesis.
d. allow the spindle to interact with the plasma
membrane. - ans-b. attach chromosomes to the spindle.
Which of the following is a target of the
APC?
a. Cdk1
b. securin
c. separase
d. S-phase CDK inhibitor Sic1
e. a and b - ans-b. securin
Which of the following inhibits separase
activity?
a. securin
b. p21CIP
c. p53
PCB 3134 TEST 4, PCB3134 Eukaryotic
Cell Structure Practice Exam 4, PCB
3134 EXAM 4
Chromosome painting involves
a. staining chromosomes with Giemsa reagent.
b. hybridizing fluorescent probes to chromosomes.
c. hybridizing radioactive probes to chromosomes.
d. a and b - ans-b. hybridizing fluorescent probes to chromosomes.
Which of the following occurs during
anaphase A?
a. The spindle elongates.
b. Kinetochores remain attached to shortening
kinetochore microtubules.
c. Chromosomes move to the spindle equator.
d. The spindle poles move closer together. - ans-b. Kinetochores remain attached to shortening
kinetochore microtubules.
During which stage of the cell cycle is the
chromosome content of a mammalian liver cell
1n?
a. G1
b. S
c. G0
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dM
e none of the above - ans-e none of the above
Human genes/proteins that regulate the cell
cycle are most easily isolated by
a. biochemical purification.
b. complementation of Xenopus mutants.
c. complementation of yeast mutants.
d. positional cloning - ans-c. complementation of yeast mutants.
Kinetochores assemble at the
a. centrosome.
b. spindle pole.
c. telomere.
d. centromere. - ans-d. centromere.
Exit from mitosis depends upon the
degradation of
a. cohesion.
b. condensin.
c. mitotic cyclin.
d. securin.
e. separase. - ans-c. mitotic cyclin.
Separase initiates sister chromatid
segregation at anaphase by cleaving
a. APC.
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b. Cdc20.
c. cyclin B.
d. cohesin.
e. securin. - ans-d. cohesin.
Phosphorylation of Thr-161 on S. pombe
MPF
a. is required for MPF activity.
b. inhibits MPF activity.
c. is carried out by Wee1 protein.
d. b and c - ans-a. is required for MPF activity.
Which of the following histone modification
likely leads to chromatin condensation?
a. Phosphoryaltion.
b. Glycosylation.
c. Deacetylation.
d. Ubiquitination. - ans-c. Deacetylation.
All of the following can be found in
chromatin except
a. DNA.
b. histones.
c. RNA.
d. condensin. - ans-c. RNA.
All the following statements about
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heterochromatin are true except
a. It is a dark-staining area of a chromosome.
b. It is usually transcriptionally active.
c. It is often simple sequence DNA.
d. It is a region of condensed chromatin - ans-b. It is usually transcriptionally active.
In the mitotic spindle, kinetochore
microtubules function to
a. connect the spindle poles.
b. attach chromosomes to the spindle.
c. carry out cytokinesis.
d. allow the spindle to interact with the plasma
membrane. - ans-b. attach chromosomes to the spindle.
Which of the following is a target of the
APC?
a. Cdk1
b. securin
c. separase
d. S-phase CDK inhibitor Sic1
e. a and b - ans-b. securin
Which of the following inhibits separase
activity?
a. securin
b. p21CIP
c. p53
