BIOL 2051 T. Luzan Lab Final (Lab Manual) questions &
answers A+ rated
1. Temperature and
Mi- crobes -pasteurization as a way to kill microbes
-refrigeration as a way to preserve food
-heat used to cook food and kill microbes
-temperature ettect depends upon the specific microbes
2. Pasteurization gentle heating (71.5-74 degrees C or 160-165 degrees F
for 15-30
seconds) was used to eliminate organisms that cause
spoilage in a food product such as beer or wine
3. Thermophiles - grow at high temperatures (45 degrees C-80 degrees C)
-grow in hot tubs, hot springs, geysers, etc.
4. Extreme Thermophiles ideal growth temperature is 100 degrees C
5. Mesophiles - grow well at the temperature comfortable for humans
between 20-45 degrees C
-optimum growth temperature at 37 degrees C
6. Psychrophiles - grow at low temperatures (<20 degrees C)
-refrigerator or polar regions of the Earth
7. Optimum Temperature the temperature at which a microbe grows the fastest
8. UV Radiation and Microbes - penetrates poorly, so there is limited applications for
eliminating
microbes
-can be used to sterilize walls, floors, counters, and to some
extent air in hospitals, labs, and operating rooms
-can be used to achieve sterility in vaccine production if very
thin liquids are treated
-portion of sunlight that is normally screened by the ozone
layer
-can be lethal to living cells
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36
,BIOL 2051 T. Luzan Lab Final (Lab Manual) questions &
answers A+ rated
-causes thymine dimers to form within DNA
2/
36
, BIOL 2051 T. Luzan Lab Final (Lab
Manual)
Study online at https://quizlet.com/_e699ea
9. Thymine Dimer can result in mutations or death of the cells
10. Pigmentation and UV Radi- - if mutation occurs in the genes for pigment production,
could
ation result colorless colony
-can provide protection from UV radiation like melanin in
human skin
11. Lethal Mutations result in the death of the cell because that damaged DNA
cannot
yield the RNA or protein product essential for cell survival
12. pH describes the acidity or alkalinity of a solution
13. Acidophiles - grows at pH values less than 6
-Lactobacillus
-Propionibacterium acnes
-yeasts and molds
-Sabouraud's Agar growth is selective for these
organisms
14. Neutrophiles - organisms best grow at a pH7
-E. coli
-Vibrio cholerae
15. Basophiles/Alkalinophiles - prefer pH above 7
-Agrobacterium
16. Solution composed of a substance (solute) dissolved in a solvent
17. Water solvent essential for all living cells
18. Osmosis flow of water from areas of less solute to areas of more
solute
19. Hypertonic - concentration of a solute is higher outside of the cell than
3/
36
, BIOL 2051 T. Luzan Lab Final (Lab
Manual)
Study online at https://quizlet.com/_e699ea it is inside
-water flows out of the cell
4/
36
answers A+ rated
1. Temperature and
Mi- crobes -pasteurization as a way to kill microbes
-refrigeration as a way to preserve food
-heat used to cook food and kill microbes
-temperature ettect depends upon the specific microbes
2. Pasteurization gentle heating (71.5-74 degrees C or 160-165 degrees F
for 15-30
seconds) was used to eliminate organisms that cause
spoilage in a food product such as beer or wine
3. Thermophiles - grow at high temperatures (45 degrees C-80 degrees C)
-grow in hot tubs, hot springs, geysers, etc.
4. Extreme Thermophiles ideal growth temperature is 100 degrees C
5. Mesophiles - grow well at the temperature comfortable for humans
between 20-45 degrees C
-optimum growth temperature at 37 degrees C
6. Psychrophiles - grow at low temperatures (<20 degrees C)
-refrigerator or polar regions of the Earth
7. Optimum Temperature the temperature at which a microbe grows the fastest
8. UV Radiation and Microbes - penetrates poorly, so there is limited applications for
eliminating
microbes
-can be used to sterilize walls, floors, counters, and to some
extent air in hospitals, labs, and operating rooms
-can be used to achieve sterility in vaccine production if very
thin liquids are treated
-portion of sunlight that is normally screened by the ozone
layer
-can be lethal to living cells
1/
36
,BIOL 2051 T. Luzan Lab Final (Lab Manual) questions &
answers A+ rated
-causes thymine dimers to form within DNA
2/
36
, BIOL 2051 T. Luzan Lab Final (Lab
Manual)
Study online at https://quizlet.com/_e699ea
9. Thymine Dimer can result in mutations or death of the cells
10. Pigmentation and UV Radi- - if mutation occurs in the genes for pigment production,
could
ation result colorless colony
-can provide protection from UV radiation like melanin in
human skin
11. Lethal Mutations result in the death of the cell because that damaged DNA
cannot
yield the RNA or protein product essential for cell survival
12. pH describes the acidity or alkalinity of a solution
13. Acidophiles - grows at pH values less than 6
-Lactobacillus
-Propionibacterium acnes
-yeasts and molds
-Sabouraud's Agar growth is selective for these
organisms
14. Neutrophiles - organisms best grow at a pH7
-E. coli
-Vibrio cholerae
15. Basophiles/Alkalinophiles - prefer pH above 7
-Agrobacterium
16. Solution composed of a substance (solute) dissolved in a solvent
17. Water solvent essential for all living cells
18. Osmosis flow of water from areas of less solute to areas of more
solute
19. Hypertonic - concentration of a solute is higher outside of the cell than
3/
36
, BIOL 2051 T. Luzan Lab Final (Lab
Manual)
Study online at https://quizlet.com/_e699ea it is inside
-water flows out of the cell
4/
36
