Hill reaction
Independent variable
light intensity reacting he chloroplasts
samples
S.I units Dependent variable - Amount of DLPIP reduced (colour
change
Control variables
Chloroplasts of he same
species
a
chopped spinach
↳ concentration of enzymes is constant
Amount
Jamb
chloroplasts/buffer
of
concentration is
solution added to each tube
uniform
>
-
,
Amount to each tube 10cmb will be
of DLPIP added added
·
-
rate to
Oxidation
uniform due concentration
-
is
·Amountlime bom tubes be
of will
fa e
compared
solution will be
Same pH used
buffer 0 added
I
to maintain pH of 7
activity
>
-
enzyme
.
affected by changes in active site .
Method :
&
-CutSpinachleaves
anda
, peste
in chilled isolation
medium
- Filter he liquid with a muslin cloth
-
transfer to
centrifuge tubes/pre coded
Centrifuge at
high speeds for
10 minutes
-
so
chloroplasts collect or he bottom as
a .
pellet
Independent variable
light intensity reacting he chloroplasts
samples
S.I units Dependent variable - Amount of DLPIP reduced (colour
change
Control variables
Chloroplasts of he same
species
a
chopped spinach
↳ concentration of enzymes is constant
Amount
Jamb
chloroplasts/buffer
of
concentration is
solution added to each tube
uniform
>
-
,
Amount to each tube 10cmb will be
of DLPIP added added
·
-
rate to
Oxidation
uniform due concentration
-
is
·Amountlime bom tubes be
of will
fa e
compared
solution will be
Same pH used
buffer 0 added
I
to maintain pH of 7
activity
>
-
enzyme
.
affected by changes in active site .
Method :
&
-CutSpinachleaves
anda
, peste
in chilled isolation
medium
- Filter he liquid with a muslin cloth
-
transfer to
centrifuge tubes/pre coded
Centrifuge at
high speeds for
10 minutes
-
so
chloroplasts collect or he bottom as
a .
pellet
