CTBS Study Guide – Complete With Correct Solutions
Aseptic recovery techniques due to oral or canal contamination Right Ans -
Minimize talking
Cover orifices
Drape donors head and perineum
Aseptic techniques for skin during recovery Right Ans - Wash donor skin
and do surgical prep or disinfect donor
Wash as much of our own skin prior to recovery
Traffic of surgical suit Right Ans - Unrestrictive = regular clothes
Semi restrictive= everything but mask (so cover hair, shoes, and wear scrubs
(tuck scrub top into pants to prevent skin sloughing))
Restrictive = everything plus the mask
Sterile field Right Ans - Sterile field must be managed by a sterile personnel
who wears a gown and their gloves cover the gown cuffs
Sterile field is very small area: it's tops of tables and a small area of the
recovering personnel
Table level
Cartilage Right Ans - Buffer for bone
Ligament Right Ans - Connects bone to bone
Tendon Right Ans - Connects muscle to bone
Skin processing/preservation Right Ans - processing of skin is initiated
within 10 days of recovery if storage media is replaced every 72 hours.
If media isn't changed, processing is initiated within 96 hours of recovery
8 required infectious disease tests Right Ans - 1. HIV Ab to types 1 and 2
(anti HIV-1 and anti HIV-2)
2. Hep C Ab (anti HCV)
3. Hep B core Ab (anti HBc - total IgG and IGM)
,4. Hep B surface antigens (HBsAg)
5. HCV NAT
6. HIV-1 NAT
7. HBV NAT
8. Syphilis (non treponemal or treponemal specific assay)
Leukocyte rich tissue donors Right Ans - Semen/cellular tissue
Must test negative for
Human T lymphotropic virus type I and II antibodies (anti HTLV -1 and anti
HTLV-II)
Testing on living donors Right Ans - Non autologous donors require
negative
WNV NAT during June 1st through October 31 every year
Where is staphylococcus found? Right Ans - Skin, hair, and nasopharynx
Look like clumped grapes
Where is streptococcus found? Right Ans - Mouth, upper respiratory tract,
skin, and intestines
Look like Small circular and chained
Where is enterococcus found? Right Ans - GI tract
Look like Circular diplos
Where is Escherichia coli found? Right Ans - Lower intestines
Look like Large cylindricals
All tissue is quarantined until Right Ans - 1. All required infectious disease
testing is complete, reviewed, and found to be negative
2. Donor screening complete and reviewed and deemed risk free of
HIV/HBV/HCV
Tissue is quarantined if Right Ans - 1. Pending completion processing
2. Unacceptable test results
3. Recall
4. Failing QA
5. Discard as waste
6. Returned by co-signer
, Max Storage period Based on Right Ans - 1. Type of tissue
2. Method of preservation
3. Storage temp
4. Packaging
5. Processing
6. Intended application
Transplantation occurs after reviewed by whom? Right Ans - medical
director
Preliminary review Right Ans - DRAI is preliminarily reviewed to evaluate
acceptability for recovery/acquisition/collection/processing
Non-treponemal screening assay used and donor is positive for syphilis/RPR,
And treponemal specific confirmatory assay is negative
If only treponemal specific confirmatory test is used on donor and results are
positive, tissue cannot be transplanted Right Ans - Tissue can be used
Tissue cannot be used if Right Ans - only treponemal specific confirmatory
test is used on donor and results are positive
LABEL: non clinical use only and biohazard Right Ans - Goes on Tissue
ineligible for transplant due to donor eligibility assessment or disease
screening, but available for non-clinical use
Why we concerned with smallpox vaccine? Right Ans - Smallpox caused by
variola virus
The vaccine against smallpox is made from the vaccinia virus
Airborne
Live vaccine = possible active infection
Process for a donors weight? Right Ans - Use the worst case scenario,
which will be the largest
Chancroid Right Ans - bacterial STD causing genital sores
When do you not have jaundice? Right Ans - When your skin is yellowish in
color and your total bilirubin is < 3mg/dL, high bilirubin levels = jaundice
Aseptic recovery techniques due to oral or canal contamination Right Ans -
Minimize talking
Cover orifices
Drape donors head and perineum
Aseptic techniques for skin during recovery Right Ans - Wash donor skin
and do surgical prep or disinfect donor
Wash as much of our own skin prior to recovery
Traffic of surgical suit Right Ans - Unrestrictive = regular clothes
Semi restrictive= everything but mask (so cover hair, shoes, and wear scrubs
(tuck scrub top into pants to prevent skin sloughing))
Restrictive = everything plus the mask
Sterile field Right Ans - Sterile field must be managed by a sterile personnel
who wears a gown and their gloves cover the gown cuffs
Sterile field is very small area: it's tops of tables and a small area of the
recovering personnel
Table level
Cartilage Right Ans - Buffer for bone
Ligament Right Ans - Connects bone to bone
Tendon Right Ans - Connects muscle to bone
Skin processing/preservation Right Ans - processing of skin is initiated
within 10 days of recovery if storage media is replaced every 72 hours.
If media isn't changed, processing is initiated within 96 hours of recovery
8 required infectious disease tests Right Ans - 1. HIV Ab to types 1 and 2
(anti HIV-1 and anti HIV-2)
2. Hep C Ab (anti HCV)
3. Hep B core Ab (anti HBc - total IgG and IGM)
,4. Hep B surface antigens (HBsAg)
5. HCV NAT
6. HIV-1 NAT
7. HBV NAT
8. Syphilis (non treponemal or treponemal specific assay)
Leukocyte rich tissue donors Right Ans - Semen/cellular tissue
Must test negative for
Human T lymphotropic virus type I and II antibodies (anti HTLV -1 and anti
HTLV-II)
Testing on living donors Right Ans - Non autologous donors require
negative
WNV NAT during June 1st through October 31 every year
Where is staphylococcus found? Right Ans - Skin, hair, and nasopharynx
Look like clumped grapes
Where is streptococcus found? Right Ans - Mouth, upper respiratory tract,
skin, and intestines
Look like Small circular and chained
Where is enterococcus found? Right Ans - GI tract
Look like Circular diplos
Where is Escherichia coli found? Right Ans - Lower intestines
Look like Large cylindricals
All tissue is quarantined until Right Ans - 1. All required infectious disease
testing is complete, reviewed, and found to be negative
2. Donor screening complete and reviewed and deemed risk free of
HIV/HBV/HCV
Tissue is quarantined if Right Ans - 1. Pending completion processing
2. Unacceptable test results
3. Recall
4. Failing QA
5. Discard as waste
6. Returned by co-signer
, Max Storage period Based on Right Ans - 1. Type of tissue
2. Method of preservation
3. Storage temp
4. Packaging
5. Processing
6. Intended application
Transplantation occurs after reviewed by whom? Right Ans - medical
director
Preliminary review Right Ans - DRAI is preliminarily reviewed to evaluate
acceptability for recovery/acquisition/collection/processing
Non-treponemal screening assay used and donor is positive for syphilis/RPR,
And treponemal specific confirmatory assay is negative
If only treponemal specific confirmatory test is used on donor and results are
positive, tissue cannot be transplanted Right Ans - Tissue can be used
Tissue cannot be used if Right Ans - only treponemal specific confirmatory
test is used on donor and results are positive
LABEL: non clinical use only and biohazard Right Ans - Goes on Tissue
ineligible for transplant due to donor eligibility assessment or disease
screening, but available for non-clinical use
Why we concerned with smallpox vaccine? Right Ans - Smallpox caused by
variola virus
The vaccine against smallpox is made from the vaccinia virus
Airborne
Live vaccine = possible active infection
Process for a donors weight? Right Ans - Use the worst case scenario,
which will be the largest
Chancroid Right Ans - bacterial STD causing genital sores
When do you not have jaundice? Right Ans - When your skin is yellowish in
color and your total bilirubin is < 3mg/dL, high bilirubin levels = jaundice
