Central dogma - Answers DNA > RNA > protein
2 main stages of protein synthesis - Answers transcription, translation
transcription - Answers DNA > mRNA
information in DNA (template strand) transcribed into mRNA
enzyme: RNA polymerase
structure of rna - Answers single-stranded
sugar = Ribose
Uracil instead of Thymine (A, U, G, C)
types of rna - Answers mRNA and tRNA
mRNA - Answers copy of gene - goes to ribosome
tRNA - Answers carries amino acids to ribosomes
transcription factors - Answers proteins that bind dna at specific sites, initiate transcription, bind to
promoter region on DNA (start of gene), RNA polymerase binds to make mRNA
steps of transcription - Answers initiation, elongation, termination
initiation - Answers binding of transcription factors and RNA polymerase to promoter
elongation - Answers rna polymerase adds nucleotides in a 5' to 3' direction
termination - Answers terminator sequence is reached
RNA processing - Answers before leaving nucleus - mRNA is processed
5' cap
3' poly a tail
removal of introns (intervening sequences - don't code for protein)
translation - Answers mRNA > protein
information in mRNA translated into protein
occurs at ribosome
uses genetic code
, genetic code - Answers triplet code (3 mRNA bases = 1 amino acid)
non-overlapping
redundant - more than one codon can specify an amino acid
unambiguous
universal
steps of translation - Answers initiation, elongation, termination
initiation - Answers mRNA, small ribosomal unit, and tRNA come together
elongation - Answers large subunit attaches, tRNA molecules bring amino acid to ribosome, amino acid
attached
termination - Answers stop codon reached
how does trna know which amino acid to deliver? - Answers trna: anticodon complementary to condon
on mrna
protein structure - Answers 4 levels of protein structure = 3D shape
protein function is dependent upon proper structure
change in structure leads to change in function
primary structure - Answers the sequence of amino acids
secondary structure - Answers folding into loops, coils, helices, sheets
dependent on primary structure
tertiary structure - Answers further folding as a result of interactions with water (hydrophobic regions
avoid water, hydrophilic regions orient towards water)
quarternary structure - Answers joining together of more than one polypeptide
epigenetic - Answers "above the gene"
epigenetic changes - Answers - do not change DNA sequences
- do change chemical groups associated w/ DNA
- can be passed to new generation
embryo subunits - Answers epsilon and zeta
fetus subunits - Answers gamma and alpha