Clinical Microbiology Exam Questions and Correct Solutions (Rated A+) Already Passed
Other study sites I have found helpful (I am not advertising for them, I just found them helpful): -
Answers http://quizlet.com/19112997/clinical-microbiology-test-1-study-guide-flash-cards/
http://quizlet.com/32297947/microbiology-i-media-biochemical-tests-for-enterobacteriaceae-flash-
cards/
http://quizlet.com/20801529/0515-bacteria-fungi-viruses-flow-charts-flash-cards/
http://www.microbiologyinpictures.com/index.html
http://www.uiweb.uidaho.edu/micro_biology/250/IDFlowcharts.pdf
http://www.austincc.edu/microbugz/lab_exercises.php
Define the term Microbiology - Answers The study of microorganisms and their relationships to the
environment, humans and animals in disease
Name the 5 classifications of microorganisms - Answers Bacteria, Virus, Mycobacterium, Fungi, Parasites
Blood agar is the most commonly used media in the clinical laboratory. What type of media is blood
agar? - Answers Enriched media, Differential media (detection of Beta-hemolytic organisms)
List some agars that are generally used in stool cultures and what type they are - Answers BAP -
Enriched, Differential
MAC - Selective, Differential
Campy - Enriched, Selective
Hektoen Enteric (HE) - Selective, Differential
Mac Sorbitol - Enriched, Selective
What media is used for Funal cultures? - Answers Sabouraud's (Sabs)
Inhibitory Mold (IMA)
,Brain Heart Infusion (BHI)
What media is generally used for the cultivation of acid fast bacilli? - Answers 7H11
7H11 Selective
Explain the difference between Prokaryote and Eukaryote - Answers Prokaryotes are bacteria that has
free floating RNA and DNA floating within the cell whereas Eukaryotes have a true nucleus surrounded
by a nuclear membrane
What are the 3 basic shapes of bacterium - Answers Cocci, Bacilli and Spirilla
List the growth factor/conditions in order to grow bacteria in the lab - Answers Atmosphere: Aerobic,
Anaerobic, Facultative Anaerobic conditions
Temperature: Psychrophiles, Mesophiles, Thermophiles
pH: Acidic vs Alkalinity environment
Osmotic Pressure: Isotonic conditions
What are the 4 stages of the Microbial Growth Curve - Answers Lag Phase: Introduction of bacteria into
the environment
Log (Exponential) Phase: Grow and multiply i.e.] 2,4,8,16,32,64,128 . . .
Stationary Phase: When the nutrients are depleting and toxic by-products accumulate
Decline (Death) Phase: Nutrients are totally depleted
What is the difference between Normal and Transient Flora? - Answers Normal flora are microorganisms
that are permanent residents in certain parts of the body. Transient flora are organisms that we
encounter everyday but do not attach permanently
What normal flora is found on the skin? - Answers Staph epidermidis
Staph aureus
, Propionibacterium acnes (anaerobic)
Corynebacterium
List some of the enterobacteriaceae organisms - Answers Salmonella
Shigella
Proteus
E. coli
Klebsiella
Enterobacter
Yersinia
Serratia
List some normal flora organisms located in the mouth - Answers Staph epi
Staph aureus
Strept mitis
Stept salivarius
Strept pneumo
Neisseria species - N meningitidis
Lactobacillus species
Spirochetes
What is the main difference between Gram + and Gram = organisms? - Answers Purple vs Red . . .
Gram + bacteria have a thick layer of peptidoglycan that Crystal Violet stain penetrates. A gram =
bacterium has an outer membrane covering a thin layer of peptidoglycan, Safranin or Carbol fuchsin
penetrates
Describe how to ID: Staph aureus - Answers Gram + cocci (clusters):
Catalase +
Coag +
Other study sites I have found helpful (I am not advertising for them, I just found them helpful): -
Answers http://quizlet.com/19112997/clinical-microbiology-test-1-study-guide-flash-cards/
http://quizlet.com/32297947/microbiology-i-media-biochemical-tests-for-enterobacteriaceae-flash-
cards/
http://quizlet.com/20801529/0515-bacteria-fungi-viruses-flow-charts-flash-cards/
http://www.microbiologyinpictures.com/index.html
http://www.uiweb.uidaho.edu/micro_biology/250/IDFlowcharts.pdf
http://www.austincc.edu/microbugz/lab_exercises.php
Define the term Microbiology - Answers The study of microorganisms and their relationships to the
environment, humans and animals in disease
Name the 5 classifications of microorganisms - Answers Bacteria, Virus, Mycobacterium, Fungi, Parasites
Blood agar is the most commonly used media in the clinical laboratory. What type of media is blood
agar? - Answers Enriched media, Differential media (detection of Beta-hemolytic organisms)
List some agars that are generally used in stool cultures and what type they are - Answers BAP -
Enriched, Differential
MAC - Selective, Differential
Campy - Enriched, Selective
Hektoen Enteric (HE) - Selective, Differential
Mac Sorbitol - Enriched, Selective
What media is used for Funal cultures? - Answers Sabouraud's (Sabs)
Inhibitory Mold (IMA)
,Brain Heart Infusion (BHI)
What media is generally used for the cultivation of acid fast bacilli? - Answers 7H11
7H11 Selective
Explain the difference between Prokaryote and Eukaryote - Answers Prokaryotes are bacteria that has
free floating RNA and DNA floating within the cell whereas Eukaryotes have a true nucleus surrounded
by a nuclear membrane
What are the 3 basic shapes of bacterium - Answers Cocci, Bacilli and Spirilla
List the growth factor/conditions in order to grow bacteria in the lab - Answers Atmosphere: Aerobic,
Anaerobic, Facultative Anaerobic conditions
Temperature: Psychrophiles, Mesophiles, Thermophiles
pH: Acidic vs Alkalinity environment
Osmotic Pressure: Isotonic conditions
What are the 4 stages of the Microbial Growth Curve - Answers Lag Phase: Introduction of bacteria into
the environment
Log (Exponential) Phase: Grow and multiply i.e.] 2,4,8,16,32,64,128 . . .
Stationary Phase: When the nutrients are depleting and toxic by-products accumulate
Decline (Death) Phase: Nutrients are totally depleted
What is the difference between Normal and Transient Flora? - Answers Normal flora are microorganisms
that are permanent residents in certain parts of the body. Transient flora are organisms that we
encounter everyday but do not attach permanently
What normal flora is found on the skin? - Answers Staph epidermidis
Staph aureus
, Propionibacterium acnes (anaerobic)
Corynebacterium
List some of the enterobacteriaceae organisms - Answers Salmonella
Shigella
Proteus
E. coli
Klebsiella
Enterobacter
Yersinia
Serratia
List some normal flora organisms located in the mouth - Answers Staph epi
Staph aureus
Strept mitis
Stept salivarius
Strept pneumo
Neisseria species - N meningitidis
Lactobacillus species
Spirochetes
What is the main difference between Gram + and Gram = organisms? - Answers Purple vs Red . . .
Gram + bacteria have a thick layer of peptidoglycan that Crystal Violet stain penetrates. A gram =
bacterium has an outer membrane covering a thin layer of peptidoglycan, Safranin or Carbol fuchsin
penetrates
Describe how to ID: Staph aureus - Answers Gram + cocci (clusters):
Catalase +
Coag +
