Microbiology UTA 2460 - Lab
Practical 2 Exam Q’s and A’s
What is the role of resazurin in fluid thioglycolate medium... - -Resazurin
indicates the presence of oxygen in the media
- A bacterial isolate is subcultured to two blood agar plates, one of which is
incubated in an anaerobe jar, and the other is incubated on a shelf in the
incubator. After incubation, growth is observed on the plate from the
anaerobe jar, but there is not growth on the plate incubated on the incubator
shelf. Based on these results how would this organism be categorized... - -
obligate anaerobe
- Where would you predict an facultative anaerobe would grow in a tube of
fluid thioglycolate medium... - -throughout the tube
- The pink color in the top of the broth in an FTM tube indicates... - -the
presence of oxygen
- Both human pathogens and normal microbiota are typically classified as... -
-mesophiles
- Taq polymersae is a DNA polymerase enzyme that demonstrates maximal
activity at 72C. The organism from which this enzyme was isolated would
most likely be classified as a... - -thermophile
- Vibrio cholerae is the bacterium responsible for cholera, a GI tract
infection. Normally, it takes the ingestion of over one million bacterial cells to
cause infection, but the consumption of antacids significantly lowers this
number. What does this tell you about the likely classification of V. cholerae
in terms of pH preference... - -it is likely an alkaliphile
- How does pH negatively affect the metabolism of microorganisms... - -pH
changes can denature enzymes
- Why can jams and honey be stored at room temperature for long periods
of time... - -the high sugar content causes plasmolysis of bacterial cells
- Microorganisms that can grow at salt concentrations as high as 15-30% are
known as... - -halophiles
- An experiment involves the inoculation bacteria onto a variety of media
with NaCl concentrations ranging from 1% to 25%. Which of these media is
most likely to be isotonic with the bacterial cells... - -1%
Practical 2 Exam Q’s and A’s
What is the role of resazurin in fluid thioglycolate medium... - -Resazurin
indicates the presence of oxygen in the media
- A bacterial isolate is subcultured to two blood agar plates, one of which is
incubated in an anaerobe jar, and the other is incubated on a shelf in the
incubator. After incubation, growth is observed on the plate from the
anaerobe jar, but there is not growth on the plate incubated on the incubator
shelf. Based on these results how would this organism be categorized... - -
obligate anaerobe
- Where would you predict an facultative anaerobe would grow in a tube of
fluid thioglycolate medium... - -throughout the tube
- The pink color in the top of the broth in an FTM tube indicates... - -the
presence of oxygen
- Both human pathogens and normal microbiota are typically classified as... -
-mesophiles
- Taq polymersae is a DNA polymerase enzyme that demonstrates maximal
activity at 72C. The organism from which this enzyme was isolated would
most likely be classified as a... - -thermophile
- Vibrio cholerae is the bacterium responsible for cholera, a GI tract
infection. Normally, it takes the ingestion of over one million bacterial cells to
cause infection, but the consumption of antacids significantly lowers this
number. What does this tell you about the likely classification of V. cholerae
in terms of pH preference... - -it is likely an alkaliphile
- How does pH negatively affect the metabolism of microorganisms... - -pH
changes can denature enzymes
- Why can jams and honey be stored at room temperature for long periods
of time... - -the high sugar content causes plasmolysis of bacterial cells
- Microorganisms that can grow at salt concentrations as high as 15-30% are
known as... - -halophiles
- An experiment involves the inoculation bacteria onto a variety of media
with NaCl concentrations ranging from 1% to 25%. Which of these media is
most likely to be isotonic with the bacterial cells... - -1%
