Genetics Test 4 Questions and Answers
LCs
Restriction enzymes cleave _____ bonds in DNA. - Answer-phosphodiester
True or False: Genetically modified organisms are typically transgenic organisms. - Answer-true
A graduate student wants to amplify a gene of interest using polymerase chain reaction. When her PCR
protocol is complete, she is surprised to find that four genes, rather than just one, were amplified. How
is this possible? - Answer-The student likely made a mistake in designing her primers.
In a Southern blot, the probe for a specific DNA sequence is incubated with a(n) _____ that has size-
separated DNA attached to it. - Answer-membrane
The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the
palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this
restriction enzyme - Answer-3′-CTTAAG-5′
Which of the donor DNAs shown below have been cut with a restriction enzyme that produces sticky
ends?
The original DNA sequence:
, GCTAATTGCACCATTAAGC
CGATTAACGTGGTAATTCG
CGATTAACGTGGT---------- - Answer----AATTGCACCA-----------
During DNA cloning using bacterial cells, which of the following types of DNA is amplified? - Answer-
both donor and vector DNA
A researcher wants to use electrophoresis and a probe to determine whether cancer cells synthesize a
specific protein molecule. This researcher should use a(n) - Answer-Western blot.
Which of the following statements is TRUE?
Please choose the correct answer from the following choices, and then select the submit answer button.
a) The gene encoding human insulin contains only exons; it completely lacks introns.
b) A researcher who wished to isolate insulin RNA from a patient could take a tissue sample, preferably
one including β-islet cells, from the pancreas.
c) A researcher who wanted to produce human insulin using bacterial cells would likely insert the full
human insulin gene, not cDNA derived from this gene, into a vector.
d) The exact genomic location of the human insulin gene is not known; thus, polymerase chain reaction
could never be used to amplify this gene.
e) None of the answers is correct. - Answer-c) A researcher who wished to isolate insulin RNA from a
patient could take a tissue sample, preferably one including β-islet cells, from the pancreas.
If you were to take a cross section of an agarose or electrophoretic gel, you would see that it contains
many small pores, or air pockets; DNA molecules must navigate around such pockets as these molecules
migrate toward the end of the gel. The presence of these pores in the agarose gel supports the analogy
that an agarose gel is like a(n) - Answer-sieve.
LCs
Restriction enzymes cleave _____ bonds in DNA. - Answer-phosphodiester
True or False: Genetically modified organisms are typically transgenic organisms. - Answer-true
A graduate student wants to amplify a gene of interest using polymerase chain reaction. When her PCR
protocol is complete, she is surprised to find that four genes, rather than just one, were amplified. How
is this possible? - Answer-The student likely made a mistake in designing her primers.
In a Southern blot, the probe for a specific DNA sequence is incubated with a(n) _____ that has size-
separated DNA attached to it. - Answer-membrane
The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the
palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this
restriction enzyme - Answer-3′-CTTAAG-5′
Which of the donor DNAs shown below have been cut with a restriction enzyme that produces sticky
ends?
The original DNA sequence:
, GCTAATTGCACCATTAAGC
CGATTAACGTGGTAATTCG
CGATTAACGTGGT---------- - Answer----AATTGCACCA-----------
During DNA cloning using bacterial cells, which of the following types of DNA is amplified? - Answer-
both donor and vector DNA
A researcher wants to use electrophoresis and a probe to determine whether cancer cells synthesize a
specific protein molecule. This researcher should use a(n) - Answer-Western blot.
Which of the following statements is TRUE?
Please choose the correct answer from the following choices, and then select the submit answer button.
a) The gene encoding human insulin contains only exons; it completely lacks introns.
b) A researcher who wished to isolate insulin RNA from a patient could take a tissue sample, preferably
one including β-islet cells, from the pancreas.
c) A researcher who wanted to produce human insulin using bacterial cells would likely insert the full
human insulin gene, not cDNA derived from this gene, into a vector.
d) The exact genomic location of the human insulin gene is not known; thus, polymerase chain reaction
could never be used to amplify this gene.
e) None of the answers is correct. - Answer-c) A researcher who wished to isolate insulin RNA from a
patient could take a tissue sample, preferably one including β-islet cells, from the pancreas.
If you were to take a cross section of an agarose or electrophoretic gel, you would see that it contains
many small pores, or air pockets; DNA molecules must navigate around such pockets as these molecules
migrate toward the end of the gel. The presence of these pores in the agarose gel supports the analogy
that an agarose gel is like a(n) - Answer-sieve.
