BIOL 200 Questions with Verified
Solutions
Suppose you successfully transplant an entire gene from a eukaryotic cell into a prokaryotic cell.
Can you be sure that the prokaryotic mRNA will be the same as the mRNA produced by the
eukaryotic cell (ignoring the 5′ cap and 3′ tail)? ✔️✔️no, because the gene could include
introns, which would not be removed in the prokaryote
where is the 5' end ✔️✔️furthest away from RNA polymerase
if each base in an mRNA sequence were translated into one amino acid in a polypeptide, for how
many types of amino acids could mRNA code? ✔️✔️4; there are 4 bases in mRNA, if each
translates to one amino acid, there would be four possible choices of amino acids
if each unique doublet of two bases in the mRNA sequence were translated into one type of
amino acid, would there be a sufficient number of unique two base sequences to represent all 20
amino acids ? ✔️✔️no, this would lead 4^2=16, which is not 20.
what must RNA have in order to be translated? ✔️✔️indication of where to start translation
indication of polypeptides amino acid sequence
indication of where to stop translation
where are codons located? ✔️✔️mRNA
, is tRNA apart of mRNA? ✔️✔️no
how are amino acids matched to bases in the mRNA sequences? ✔️✔️molecules of tRNA are
used to match each triplet of bases in the mRNA to an amino acid
what is difference between the promoter and the ribosome binding site? ✔️✔️the promoter is
on DNA, the ribosome binding site is on mRNA
in what binding sites are the tRNAs never attached? ✔️✔️E
how does the ribosome move along the mRNA after incorporating an amino acid? ✔️✔️the
ribosome moves 3 bases along the mRNA
the peptide bond is formed between amino acids attached to tRNAs at which two sites?
✔️✔️A&P
is the stop codon part of the open reading frame? ✔️✔️no
during translation initiation, what happens after the small ribosomal subunit binds? ✔️✔️the
tRNA carrying methionine binds to the mRNA
Solutions
Suppose you successfully transplant an entire gene from a eukaryotic cell into a prokaryotic cell.
Can you be sure that the prokaryotic mRNA will be the same as the mRNA produced by the
eukaryotic cell (ignoring the 5′ cap and 3′ tail)? ✔️✔️no, because the gene could include
introns, which would not be removed in the prokaryote
where is the 5' end ✔️✔️furthest away from RNA polymerase
if each base in an mRNA sequence were translated into one amino acid in a polypeptide, for how
many types of amino acids could mRNA code? ✔️✔️4; there are 4 bases in mRNA, if each
translates to one amino acid, there would be four possible choices of amino acids
if each unique doublet of two bases in the mRNA sequence were translated into one type of
amino acid, would there be a sufficient number of unique two base sequences to represent all 20
amino acids ? ✔️✔️no, this would lead 4^2=16, which is not 20.
what must RNA have in order to be translated? ✔️✔️indication of where to start translation
indication of polypeptides amino acid sequence
indication of where to stop translation
where are codons located? ✔️✔️mRNA
, is tRNA apart of mRNA? ✔️✔️no
how are amino acids matched to bases in the mRNA sequences? ✔️✔️molecules of tRNA are
used to match each triplet of bases in the mRNA to an amino acid
what is difference between the promoter and the ribosome binding site? ✔️✔️the promoter is
on DNA, the ribosome binding site is on mRNA
in what binding sites are the tRNAs never attached? ✔️✔️E
how does the ribosome move along the mRNA after incorporating an amino acid? ✔️✔️the
ribosome moves 3 bases along the mRNA
the peptide bond is formed between amino acids attached to tRNAs at which two sites?
✔️✔️A&P
is the stop codon part of the open reading frame? ✔️✔️no
during translation initiation, what happens after the small ribosomal subunit binds? ✔️✔️the
tRNA carrying methionine binds to the mRNA
