Howard MIC224 Final Practical With Solution

Howard MIC224 Final Practical With
Solution

What does MSA stand for? - ANSWER mannitol salt agar

What is the substrate in MSA? - ANSWER mannitol

What is the indicatior in MSA? - ANSWER phenol red

What's the selective agent in MSA? - ANSWER NaCl

What color would a positive reaction be on MSA? - ANSWER yellow (s.
aureus)

What color would a negative reaction be on MSA? - ANSWER red (s.
epidermis)

What does GEL stand for? - ANSWER gelatin agar

Is MSA selective or differential? - ANSWER selective

What is the substrate in GEL? - ANSWER gelatin

What is the indicator in GEL? - ANSWER HCl

What is the selective agent in GEL? - ANSWER none

What does a positive reaction (zone) mean in GEL plates? - ANSWER the
organism produces gelatinase

What does STA stand for? - ANSWER starch agar

What is the substrate in STA? - ANSWER starch

, What is the indicator in STA? - ANSWER iodine

What is the selective agent in STA? - ANSWER none

Is STA selective or differential? - ANSWER differential

What does a positive reaction to STA mean? - ANSWER the organism
produces amylase

What does SBA stand for? - ANSWER sheep's blood agar

What is the substrate in SBA? - ANSWER blood

What is the indicator in SBA? - ANSWER none

What is the selective agent in SBA? - ANSWER none




Is SBA selective or differential? - ANSWER differential




What does alpha hemolysis mean? - ANSWER green, incomplete clearing




What does beta hemolysis mean? - ANSWER complete clearing, zone




What does gamma hemolysis mean? - ANSWER no clearing, nothing




What is the selective agent in PEA? - ANSWER phenol alcohol