Red Blood Cell Count Exam Study Guide Solutions

Red Blood Cell Count Exam Study Guide Solutions red blood cell count - ANSWER-is the number of red cells in 1 mm^3 of blood isotonic solution - ANSWER-blood is diluted with an _____ solution to preserve the blood corpuscles Gower's diluting fluid - ANSWER-diluting fluid used in RBC count must be an isotonic solution must have a high specific gravity must be easy to prepare must be cheap must be a good preservative must have a buffer action must not initiate growth of molds - ANSWER-Characteristics of a good RBC diluting fluid 0.5 mark - ANSWER-aspirate blood up to the ____ mark of the RBC pipette ©EMILLECT2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/8 diluting fluid - ANSWER-where to place the pipette after aspirating 0.5 mark of blood 101 mark - ANSWER-aspirate the diluting fluid up to the _____ mark 1:200 - ANSWER-dilution of RBC pipette 5-6 drops - ANSWER-number of drops to be discarded of the diluted blood from the pipette 30°-35° - ANSWER-angle of the pipette while charging LPO - ANSWER-locate the ruled area for RBC counting by focusing the microscope using the ______ HPO - ANSWER-objective used for actual counting five tertiary squares - ANSWER-count the RBCs seen on the _______ of the counting chamber 1/5 mm^2 - ANSWER-area of the five squares 20 or less - ANSWER-cell difference between the squares of RBC count 4.5-6.0 M/mm^3 - ANSWER-normal values of RBC count in males 4.0-5.5 M/mm^3 - ANSWER-normal values of RBC count in females 3.0-5.0 M/mm^3 - ANSWER-normal values of RBC count in late pregnancy 7.0 M/mm^3 - ANSWER-normal values of RBC count at birth ©EMILLECT2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 3/8 I. microscopic methods ii. automated methods iii. photoelectric methods - ANSWER-Other Methods of RBC count a. Unopette system b. Tube dilution - ANSWER-microscopic methods unopette system - ANSWER-is a convenient method of preparing diluted blood sample for cell counting unopette system - ANSWER-it uses a premeasured volume of diluting fluid in a plastic reservoir capillary pipette - ANSWER-a specific amount of blood is then collected using a _______ and mixed with the diluting fluid tube dilution - ANSWER-uses 0.02 mL of blood plus 4 mL of diluting fluid a. sysmex E-5000 b. sysmex NE-8000 - ANSWER-automated methods sysmex E-5000 - ANSWER-analyzes and reports CBC, RBC indices, red blood cell distribution with (RDW) platelet count, MVP and nucleated cells according to