BOC Study Guide Histotechnology 2nd
Ed. - Fixation (HT) Questions and
Answers Graded A+
What is the primary purpose of fixation in histotechnology?
A) To enhance staining
✔✔B) To preserve tissue morphology
C) To increase tissue size
D) To accelerate decomposition
Which of the following is a common fixative used in histology?
✔✔A) Formaldehyde
B) Ethanol
C) Acetone
D) Isopropanol
What is the optimal concentration of formaldehyde for fixation?
A) 1%
B) 5%
1
,✔✔C) 10%
D) 20%
What type of fixation preserves lipids better?
A) Formalin fixation
✔✔B) Osmium tetroxide fixation
C) Alcohol fixation
D) Picric acid fixation
What is a disadvantage of using glutaraldehyde as a fixative?
✔✔A) It can cause excessive cross-linking
B) It is less effective than formaldehyde
C) It does not penetrate tissues well
D) It is non-toxic
How long should tissues typically remain in formalin for proper fixation?
A) 30 minutes
✔✔B) 6-24 hours
2
, C) 48 hours
D) 1 hour
What happens to tissue if fixation is inadequate?
✔✔A) It may result in poor staining quality
B) It will be perfectly preserved
C) It will shrink significantly
D) It will be over-fixed
Which of the following is NOT a primary objective of fixation?
A) Preventing autolysis
✔✔B) Enhancing tissue elasticity
C) Stabilizing proteins
D) Preserving cellular architecture
What is the role of co-fixatives in histotechnology?
A) To decrease fixation time
✔✔B) To improve fixation quality
3
Ed. - Fixation (HT) Questions and
Answers Graded A+
What is the primary purpose of fixation in histotechnology?
A) To enhance staining
✔✔B) To preserve tissue morphology
C) To increase tissue size
D) To accelerate decomposition
Which of the following is a common fixative used in histology?
✔✔A) Formaldehyde
B) Ethanol
C) Acetone
D) Isopropanol
What is the optimal concentration of formaldehyde for fixation?
A) 1%
B) 5%
1
,✔✔C) 10%
D) 20%
What type of fixation preserves lipids better?
A) Formalin fixation
✔✔B) Osmium tetroxide fixation
C) Alcohol fixation
D) Picric acid fixation
What is a disadvantage of using glutaraldehyde as a fixative?
✔✔A) It can cause excessive cross-linking
B) It is less effective than formaldehyde
C) It does not penetrate tissues well
D) It is non-toxic
How long should tissues typically remain in formalin for proper fixation?
A) 30 minutes
✔✔B) 6-24 hours
2
, C) 48 hours
D) 1 hour
What happens to tissue if fixation is inadequate?
✔✔A) It may result in poor staining quality
B) It will be perfectly preserved
C) It will shrink significantly
D) It will be over-fixed
Which of the following is NOT a primary objective of fixation?
A) Preventing autolysis
✔✔B) Enhancing tissue elasticity
C) Stabilizing proteins
D) Preserving cellular architecture
What is the role of co-fixatives in histotechnology?
A) To decrease fixation time
✔✔B) To improve fixation quality
3
