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MLT Study Guide #1 with Complete Solutions

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MLT Study Guide #1 with Complete Solutions Hematology - Answer-the study of blood Average adult has how much blood? - Answer-About 5-6 liters (8-12 pints) When blood is centrifuges what happens? - Answer-It seperates into two distinct portions plasma & cells About 45% is cellular or formed elements called? - Answer-RBCs, WBCs, and platelets RBCs - Answer-Contain hemoglobin which carries oxygen to tissues WBCs - Answer-defend against foreign infectious agents (such as bacteria, viruses, fungi, etc. Platelets (Plt) - Answer-aid in the stoppage of bleeding The remaining 55% is plasma which contains what? - Answer-Straw/yellow fluid which is 90% water and 10% proteins, carbohydrates, vitamins, hormones, enzymes, lipids, salts, etc. Transmission of microorganisms from person to person resulting in infection is called? - Answer- Chain of infection What is blood-borne pathogens? - Answer-Organisms that can be transmitted through contact with human blood or other potentially infectious fluids or specimens that come from humans. HIV, HCV, and HBV are the viruses that are of major concern. EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024. Page 2/5 Universal Precautions - Answer-(UP) a method of infection control in which all human blood and other body fluids containing visible blood are treated as if infectious. Standard Precautions - Answer-a set of comprehensive safety guidelines designed to protect patients and healthcare workers by requiring that all patients and all body fluids, body substances, organs, and unfixed tissues be regarded as potentially infectious. (Combines UP &BI) BSI - Answer-Body Substance Isolation PPE - Answer-Personal Protective Equipment should be worn when handling or collecting blood or body fluids contaminated with blood. (gloves, goggles, face shield, etc) Work areas should be cleaned with what? - Answer-10% solution of bleach (1:10 dilution- 1 part bleach mixed with 9 parts water) Active ingredient in household bleach is hypochlorite and a 10% solution equals 0.5% sodium hypochlorite. What is the clear yellow colored portion called in a centrifuged blood specimen that was colllected in a vacuum tube without anticoagulant? - Answer-Serum (it does not contain fibrinogen) What is the clear yellow portion called in a blood specimen with anticoagulant? - Answer-Plasma (it contains fibrinogen) What is the correct order of draw? - Answer-(1) Blood culture tubes (2) sodium citrate -coagulation tubes- LIGHT BLUE TOP (3) Serum tubes with or without clot activators- RED, GOLD, OR SPECKLED TOP (4) -eparin tubes with or without gel-GREEN TOP (5) EDTA tubes-LAVENDER TOP (6) Sodium fluoride (glycolytic inhibitor)-GRAY TOP Order of Draw - Answer- EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024. Page 3/5 What is an anticoagulant? - Answer-Substances that prevent the clotting (coagulation) of blood. Oxalate, Citrate, and EDTA - Answer-These work by removing calcium from the coagulation process. Disodium or dipotassium (K3) salt of Ethylenediaminetetraacetic Acid or EDTA - Answer-EDTA prevents coagulation by chelating (binding) ionized calcium in the blood into an insoluble salt which is a necessary component in the coagulation process. EDTA - Answer-It is the best anticoagulant to make blood smears/films for differentials because it causes less distortion of blood cells and less artifacts on the slide. Good for hematology procedures because it prevents platelet aggregation which yields more reliable platelet counts and makes them look more natural on prepared slides. (lavender top) Blood smears/slides - Answer-Must be made within 2-3 hours after collection from the patient or distortion and artifacts will increase with time. Excessive EDTA - Answer-causes RBCs to shrink causing crenation Sodium Citrate - Answer-the anticoagulant of choice for coagulation studies. It prevents coagulation by precipitating calcium in an unusable form. Tubes must be filled properly (full) because coagulation tests can be affected if the proper 1 part 0.109 (3.2%) sodium citrate to 9 parts whole blood is not met. Also good at maintaining the stability of factors V, VIII and platelet functions. (light blue top) Heparin - Answer-coagulation is prevented by enhancing antithrombin III which in turn neutralizes factor II (thrombin) & X. It is not a good choice for preparing differential sides since WBCs and Platelets will clump in addition to the stain being to blue. For special hematology test. (green top

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EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.




MLT Study Guide #1 with Complete
Solutions

Hematology - Answer✔✔-the study of blood


Average adult has how much blood? - Answer✔✔-About 5-6 liters (8-12 pints)


When blood is centrifuges what happens? - Answer✔✔-It seperates into two distinct portions


plasma & cells


About 45% is cellular or formed elements called? - Answer✔✔-RBCs, WBCs, and platelets


RBCs - Answer✔✔-Contain hemoglobin which carries oxygen to tissues


WBCs - Answer✔✔-defend against foreign infectious agents (such as bacteria, viruses, fungi, etc.


Platelets (Plt) - Answer✔✔-aid in the stoppage of bleeding


The remaining 55% is plasma which contains what? - Answer✔✔-Straw/yellow fluid which is 90% water

and 10% proteins, carbohydrates, vitamins, hormones, enzymes, lipids, salts, etc.


Transmission of microorganisms from person to person resulting in infection is called? - Answer✔✔-

Chain of infection


What is blood-borne pathogens? - Answer✔✔-Organisms that can be transmitted through contact with

human blood or other potentially infectious fluids or specimens that come from humans. HIV, HCV, and

HBV are the viruses that are of major concern.




Page 1/5

, EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.



Universal Precautions - Answer✔✔-(UP) a method of infection control in which all human blood and

other body fluids containing visible blood are treated as if infectious.


Standard Precautions - Answer✔✔-a set of comprehensive safety guidelines designed to protect patients

and healthcare workers by requiring that all patients and all body fluids, body substances, organs, and

unfixed tissues be regarded as potentially infectious. (Combines UP &BI)


BSI - Answer✔✔-Body Substance Isolation


PPE - Answer✔✔-Personal Protective Equipment should be worn when handling or collecting blood or

body fluids contaminated with blood. (gloves, goggles, face shield, etc)


Work areas should be cleaned with what? - Answer✔✔-10% solution of bleach (1:10 dilution- 1 part

bleach mixed with 9 parts water) Active ingredient in household bleach is hypochlorite and a 10%

solution equals 0.5% sodium hypochlorite.


What is the clear yellow colored portion called in a centrifuged blood specimen that was colllected in a

vacuum tube without anticoagulant? - Answer✔✔-Serum (it does not contain fibrinogen)


What is the clear yellow portion called in a blood specimen with anticoagulant? - Answer✔✔-Plasma (it

contains fibrinogen)


What is the correct order of draw? - Answer✔✔-(1) Blood culture tubes (2) sodium citrate -coagulation

tubes- LIGHT BLUE TOP (3) Serum tubes with or without clot activators- RED, GOLD, OR SPECKLED TOP

(4) -eparin tubes with or without gel-GREEN TOP (5) EDTA tubes-LAVENDER TOP (6) Sodium fluoride

(glycolytic inhibitor)-GRAY TOP


Order of Draw - Answer✔✔-




Page 2/5

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