KMLTTB DEGREE LEVEL THEORY PAPER 1
MICROBIOLOGY PAPER
SECTION A MULTIPLE CHOICE (ANSWER ALL QUESTIONS) 20 MARKS
(Q1) Which of the following is a substitute for crystal violet used in gram-staining procedure?
(a) Methylene blue
(b) Bromocresol green
(c) Safranin
(d) Phenolpthalene
(Q2)The cooci which mostly occur in single or pairs are:
(a) Streptococci
(b) Diplococci
(c) Tetracocci
(d) None of these
(Q3) Which of the following medium are used to differentiate the colonies of Vibrio cholerae
and V. parahaemolyticus?
(a) Alkaline bile salt
(b) Thiosulphate-citrate-bile-sucrose
(c) MacConkey
(d) All of the above
(Q4) Which of the following can be identified by milk ring test?
(a) Brucellosis
(b) Salmonellosis
(c) Bovine tuberculosis
(d) All of these
(Q5) Which of the following specimen is not expected to have any bacteria in normal
conditions?
(a) Urethral swab
(b) Sputum
(c) Pus
(d) Cerebrospinal fluid
1
,(Q6) Which of the folowing methods is not suitable for sterilization of bacteriological media?
(a) Tyndillization
(b) Autoclaving
(c) Steaming at 100oC
(d) disinfecting in Lysol
(Q7) The most pathogenic Brucella spp for man is:
(a) B melitensis
(b) B abortus
(c) B suis
(d) B canis
(Q8) Which of the following disinfectant is NOT effective against viruses?
(a) Hydrogen peroxide
(b) Hypochlorite
(c) Formaldehyde
(d) methylated spirit
(Q9) Salmonella typhi is association with?
(a) Botulinum
(b) Brucellosis
(c) Amoebic dysentery
(d) Enteric fever
(Q10) Cryptococcus neoformans is an opportunistic pathogen commonly isolated from?
(a) Slool
(b) Urine
(c) CSF
(d) Blood
(Q11) Which of the following is best used for long term storage of microbial specimens when
carried out properly?
(a) Storage in a freezer at -10°C
(b) Storage in a freezer at ultra low temperatures (-70°C)
(c) Storage in a refrigerator on an agar slant
(d) Storage on a petri plate at room temperature
(Q12) The concept of putting microbes to help clean up the environment is called:
2
, (a) Pasteurization
(b) Bioremediation
(c) Fermentation
(d) Biolistics
(Q13) The nature of the poliovirus given for oral vaccination (satin vaccine) as part of the
eradication programme is:
(a) heat killed virus
(b) live attenuated strains of all three immunological types
(c) small dosage of wild-type live viruses
(d) formalin-inactivated viruses
(Q14) For most bacteria, the optimum pH for growth lies between:
(a) 6.5-7.5
(b) 3.5-4.5
(c) 4.5-5.5
(d) 5.5-6.5
(Q15) Suspension cultures consist of cells and cell aggregates, growing dispersed in:
(a) liquid medium
(b) solid nutrient medium
(c) solid or liquid medium
(d) none of these
(Q16) Which of the following is not the characteristic of a growth curve?
(a) Shows development of microbial population under relatively stable
environmental conditions
(b) Plotted with logarithmic numbers
(c) Graphs numbers of microbes versus time
(d) Each growth curve consists of four distinct phases
(Q17) The organism which obtain their energy from chemicals are designated as:
(a) Prototrophs
(b) Chemotrophs
(c) Organotrophs
(d) Autotrophs
(Q18) Which of the following media is selective for Salmonella?
(a) Thiosulphate citrate bile salt agar
3
, (b) Wilson and Blair's brilliant-green bismuth sulphite agar medium
(c) Bile salt agar
(d) All of the above
(Q19) Xylose lysine deoxycholate (XLD) agar is better selective medium than the deoxycholate
citrate agar (DCA) because:
(a) it is selective
(b) it imparts better growth
(c) it contains high salt concentration
(d) it is less inhibitory to S. Dysentriae and S. flexneri
(Q20) The viruses that attack bacteria are:
(a) Bacterial viruses
(b) . Bacterial pathogens
(c) . Bacteriophages
(d) Various
SECTION B (ANSWER ALL QUESTIONS) 30 MARKS.
(Q1) Outline post-analytical errors that would compromise the quality of work in a microbiology
laboratory (10 marks)
(Q2) describe the standard operating procedure for collecting:
(a) high vaginal swab (5 marks)
(b) urethral swab from a male client (5 marks)
(Q3) briefly describe the essential areas to be considered in implementing a comprehensive
quality assurance program in microbiology laboratory (10 marks)
.
4
MICROBIOLOGY PAPER
SECTION A MULTIPLE CHOICE (ANSWER ALL QUESTIONS) 20 MARKS
(Q1) Which of the following is a substitute for crystal violet used in gram-staining procedure?
(a) Methylene blue
(b) Bromocresol green
(c) Safranin
(d) Phenolpthalene
(Q2)The cooci which mostly occur in single or pairs are:
(a) Streptococci
(b) Diplococci
(c) Tetracocci
(d) None of these
(Q3) Which of the following medium are used to differentiate the colonies of Vibrio cholerae
and V. parahaemolyticus?
(a) Alkaline bile salt
(b) Thiosulphate-citrate-bile-sucrose
(c) MacConkey
(d) All of the above
(Q4) Which of the following can be identified by milk ring test?
(a) Brucellosis
(b) Salmonellosis
(c) Bovine tuberculosis
(d) All of these
(Q5) Which of the following specimen is not expected to have any bacteria in normal
conditions?
(a) Urethral swab
(b) Sputum
(c) Pus
(d) Cerebrospinal fluid
1
,(Q6) Which of the folowing methods is not suitable for sterilization of bacteriological media?
(a) Tyndillization
(b) Autoclaving
(c) Steaming at 100oC
(d) disinfecting in Lysol
(Q7) The most pathogenic Brucella spp for man is:
(a) B melitensis
(b) B abortus
(c) B suis
(d) B canis
(Q8) Which of the following disinfectant is NOT effective against viruses?
(a) Hydrogen peroxide
(b) Hypochlorite
(c) Formaldehyde
(d) methylated spirit
(Q9) Salmonella typhi is association with?
(a) Botulinum
(b) Brucellosis
(c) Amoebic dysentery
(d) Enteric fever
(Q10) Cryptococcus neoformans is an opportunistic pathogen commonly isolated from?
(a) Slool
(b) Urine
(c) CSF
(d) Blood
(Q11) Which of the following is best used for long term storage of microbial specimens when
carried out properly?
(a) Storage in a freezer at -10°C
(b) Storage in a freezer at ultra low temperatures (-70°C)
(c) Storage in a refrigerator on an agar slant
(d) Storage on a petri plate at room temperature
(Q12) The concept of putting microbes to help clean up the environment is called:
2
, (a) Pasteurization
(b) Bioremediation
(c) Fermentation
(d) Biolistics
(Q13) The nature of the poliovirus given for oral vaccination (satin vaccine) as part of the
eradication programme is:
(a) heat killed virus
(b) live attenuated strains of all three immunological types
(c) small dosage of wild-type live viruses
(d) formalin-inactivated viruses
(Q14) For most bacteria, the optimum pH for growth lies between:
(a) 6.5-7.5
(b) 3.5-4.5
(c) 4.5-5.5
(d) 5.5-6.5
(Q15) Suspension cultures consist of cells and cell aggregates, growing dispersed in:
(a) liquid medium
(b) solid nutrient medium
(c) solid or liquid medium
(d) none of these
(Q16) Which of the following is not the characteristic of a growth curve?
(a) Shows development of microbial population under relatively stable
environmental conditions
(b) Plotted with logarithmic numbers
(c) Graphs numbers of microbes versus time
(d) Each growth curve consists of four distinct phases
(Q17) The organism which obtain their energy from chemicals are designated as:
(a) Prototrophs
(b) Chemotrophs
(c) Organotrophs
(d) Autotrophs
(Q18) Which of the following media is selective for Salmonella?
(a) Thiosulphate citrate bile salt agar
3
, (b) Wilson and Blair's brilliant-green bismuth sulphite agar medium
(c) Bile salt agar
(d) All of the above
(Q19) Xylose lysine deoxycholate (XLD) agar is better selective medium than the deoxycholate
citrate agar (DCA) because:
(a) it is selective
(b) it imparts better growth
(c) it contains high salt concentration
(d) it is less inhibitory to S. Dysentriae and S. flexneri
(Q20) The viruses that attack bacteria are:
(a) Bacterial viruses
(b) . Bacterial pathogens
(c) . Bacteriophages
(d) Various
SECTION B (ANSWER ALL QUESTIONS) 30 MARKS.
(Q1) Outline post-analytical errors that would compromise the quality of work in a microbiology
laboratory (10 marks)
(Q2) describe the standard operating procedure for collecting:
(a) high vaginal swab (5 marks)
(b) urethral swab from a male client (5 marks)
(Q3) briefly describe the essential areas to be considered in implementing a comprehensive
quality assurance program in microbiology laboratory (10 marks)
.
4
