Modern Blood Banking & Transfusion
Practices - Denise Harmening
What is the maximum volume of blood that can be collected from a 110-lb donor,
including samples for processing?
a. 450 mL
b. 500 mL
c. 525 mL
d. 550 mL - ANSWER c. 525 mL
When RBCs are stored, there is a "shift to the left." This means:
a. Hemoglobin oxygen affinity increases, owing to an increase in 2,3-DPG.
b. Hemoglobin oxygen affinity increases, owing to a decrease in 2,3-DPG.
c. Hemoglobin oxygen affinity decreases, owing to a decrease in 2,3-DPG.
d. Hemoglobin oxygen affinity decreases, owing to an increase in 2,3-DPG. -
ANSWER b. Hemoglobin oxygen affinity increases, owing to a decrease in 2,3-
DPG.
Which of the following anticoagulant preservatives provides a storage time of 35
days at 1°C to 6°C for units of whole blood and prepared RBCs if an additive solution
is not added?
a. ACD-A
b. CP2D
c. CPD
d. CPDA-1 - ANSWER d. CPDA-1
What are the current storage time and storage temperature for platelet concentrates
and apheresis platelet components?
a. 5 days at 1°C to 6°C
b. 5 days at 24°C to 27°C
c. 5 days at 20°C to 24°C
d. 7 days at 22°C to 24°C - ANSWER c. 5 days at 20°C to 24°C
With continuous agitation
Maintain vascular integrity
Initial arrest of bleeding
Stabilization of hemostatic plug - ANSWER Role of platelets
Factors influencing platelet metabolism and function in a closed system except:
,a. Total platelet count
b. Duration of storage
c. Temperature of storage
d. Fibrinogen concentration - ANSWER d. Fibrinogen concentration
Platelet components are stored up fo 5 days at 20-24'C with continuous agitation.
When necessary during shipping, platelets can be stored without agitation for up to
_____ (at 20 to 24'C) during a 5-day storage period
a. 4 hrs
b. 6 hrs
c. 12 hrs
d. 24 hrs - ANSWER d. 24 hrs
Platelets stored ar (1-6'C): 2 days
If a platelet bag is broken or opened, the platelets must be transfused within _____
when stored at 20 to 24'C
a. 4 hrs
b. 24 hrs
c. 5 days
d. 7 days - ANSWER a. 4 hrs
What is the minimum number of platelets required in a platelet concentrate prepared
from whole blood by centrifugation (90% of sampled units)?
a. 5.5 x 10^11
b. 3 x 10^10
c. 3 x 10^11
d. 5.5 x 10^10 - ANSWER d. 5.5 x 10^10
RBCs can be frozen for:
a. 12 months.
b. 1 year.
c. 5 years.
d. 10 years. - ANSWER d. 10 years
What is the minimum number of platelets required in an apheresis component (90%
of the sampled units)?
a. 3 x 10^11
b. 4 x 10^11
c. 2 x 10^11
d. 3.5 x 10^11 - ANSWER a. 3 x 10^11
Whole blood and RBC units are stored at what temperature?
,a. 1°C to 6°C
b. 20°C to 24°C
c. 37°C
d. 24°C to 27°C - ANSWER a. 1°C to 6°C
Additive solutions are approved for storage of red blood cells for how many days?
a. 21
b. 42
c. 35
d. 7 - ANSWER b. 42
One criterion used by the FDA for approval of new preservation solutions and
storage containers is an average 24-hour post-transfusion RBC survival of more
than:
a. 50%.
b. 60%.
c. 65%.
d. 75%. - ANSWER d. 75%
What is the lowest allowable pH for a platelet component at outdate?
a. 6
b. 5.9
c. 6.8
d. 6.2 - ANSWER d. 6.2
Frozen and thawed RBCs processed in an open system can be stored for how many
days/hours?
a. 3 days
b. 6 hours
c. 24 hours
d. 15 days - ANSWER c. 24 hours
What is the hemoglobin source for hemoglobin-based oxygen carriers in advanced
clinical testing?
a. Only bovine hemoglobin
b. Only human hemoglobin
c. Both bovine and human hemoglobins
d. None of the above - ANSWER c. Both bovine and human hemoglobins
Major disadvantage of stroma-free hemoglobin solutions - ANSWER High oxygen
affinity
Which of the following is the most common cause of bacterial contamination of
platelet products?
, a. Entry of skin plugs into the collection bag
b. Environmental contamination during processing
c. Bacteremia in the donor
d. Incorrect storage temperature - ANSWER a. Entry of skin plugs into the collection
bag
Which of the following is not involved in the acquired or adaptive immune response?
a. Phagocytosis
b. Production of antibody or complement
c. Induction of immunologic memory
d. Accelerated immune response upon subsequent exposure to antigen - ANSWER
a. Phagocytosis
Which cells are involved in the production of antibodies?
a. Dendritic cells
b. T lymphocytes
c. B lymphocytes
d. Macrophages - ANSWER c. B lymphocytes
Which of the following cells is involved in antigen recognition following phagocytosis?
a. B lymphocytes
b. T lymphocytes
c. Macrophages
d. Granulocytes - ANSWER b. T lymphocytes
The role of the macrophage during an antibody response is to:
a. Make antibody
b. Lyse virus-infected target cells
c. Activate cytotoxic T cells
d. Process antigen and present it - ANSWER d. Process antigen and present it
Which of the following immunoglobulins is produced in the primary immune
response?
a. IgA
b. IgE
c. IgG
d. IgM - ANSWER d. IgM
Practices - Denise Harmening
What is the maximum volume of blood that can be collected from a 110-lb donor,
including samples for processing?
a. 450 mL
b. 500 mL
c. 525 mL
d. 550 mL - ANSWER c. 525 mL
When RBCs are stored, there is a "shift to the left." This means:
a. Hemoglobin oxygen affinity increases, owing to an increase in 2,3-DPG.
b. Hemoglobin oxygen affinity increases, owing to a decrease in 2,3-DPG.
c. Hemoglobin oxygen affinity decreases, owing to a decrease in 2,3-DPG.
d. Hemoglobin oxygen affinity decreases, owing to an increase in 2,3-DPG. -
ANSWER b. Hemoglobin oxygen affinity increases, owing to a decrease in 2,3-
DPG.
Which of the following anticoagulant preservatives provides a storage time of 35
days at 1°C to 6°C for units of whole blood and prepared RBCs if an additive solution
is not added?
a. ACD-A
b. CP2D
c. CPD
d. CPDA-1 - ANSWER d. CPDA-1
What are the current storage time and storage temperature for platelet concentrates
and apheresis platelet components?
a. 5 days at 1°C to 6°C
b. 5 days at 24°C to 27°C
c. 5 days at 20°C to 24°C
d. 7 days at 22°C to 24°C - ANSWER c. 5 days at 20°C to 24°C
With continuous agitation
Maintain vascular integrity
Initial arrest of bleeding
Stabilization of hemostatic plug - ANSWER Role of platelets
Factors influencing platelet metabolism and function in a closed system except:
,a. Total platelet count
b. Duration of storage
c. Temperature of storage
d. Fibrinogen concentration - ANSWER d. Fibrinogen concentration
Platelet components are stored up fo 5 days at 20-24'C with continuous agitation.
When necessary during shipping, platelets can be stored without agitation for up to
_____ (at 20 to 24'C) during a 5-day storage period
a. 4 hrs
b. 6 hrs
c. 12 hrs
d. 24 hrs - ANSWER d. 24 hrs
Platelets stored ar (1-6'C): 2 days
If a platelet bag is broken or opened, the platelets must be transfused within _____
when stored at 20 to 24'C
a. 4 hrs
b. 24 hrs
c. 5 days
d. 7 days - ANSWER a. 4 hrs
What is the minimum number of platelets required in a platelet concentrate prepared
from whole blood by centrifugation (90% of sampled units)?
a. 5.5 x 10^11
b. 3 x 10^10
c. 3 x 10^11
d. 5.5 x 10^10 - ANSWER d. 5.5 x 10^10
RBCs can be frozen for:
a. 12 months.
b. 1 year.
c. 5 years.
d. 10 years. - ANSWER d. 10 years
What is the minimum number of platelets required in an apheresis component (90%
of the sampled units)?
a. 3 x 10^11
b. 4 x 10^11
c. 2 x 10^11
d. 3.5 x 10^11 - ANSWER a. 3 x 10^11
Whole blood and RBC units are stored at what temperature?
,a. 1°C to 6°C
b. 20°C to 24°C
c. 37°C
d. 24°C to 27°C - ANSWER a. 1°C to 6°C
Additive solutions are approved for storage of red blood cells for how many days?
a. 21
b. 42
c. 35
d. 7 - ANSWER b. 42
One criterion used by the FDA for approval of new preservation solutions and
storage containers is an average 24-hour post-transfusion RBC survival of more
than:
a. 50%.
b. 60%.
c. 65%.
d. 75%. - ANSWER d. 75%
What is the lowest allowable pH for a platelet component at outdate?
a. 6
b. 5.9
c. 6.8
d. 6.2 - ANSWER d. 6.2
Frozen and thawed RBCs processed in an open system can be stored for how many
days/hours?
a. 3 days
b. 6 hours
c. 24 hours
d. 15 days - ANSWER c. 24 hours
What is the hemoglobin source for hemoglobin-based oxygen carriers in advanced
clinical testing?
a. Only bovine hemoglobin
b. Only human hemoglobin
c. Both bovine and human hemoglobins
d. None of the above - ANSWER c. Both bovine and human hemoglobins
Major disadvantage of stroma-free hemoglobin solutions - ANSWER High oxygen
affinity
Which of the following is the most common cause of bacterial contamination of
platelet products?
, a. Entry of skin plugs into the collection bag
b. Environmental contamination during processing
c. Bacteremia in the donor
d. Incorrect storage temperature - ANSWER a. Entry of skin plugs into the collection
bag
Which of the following is not involved in the acquired or adaptive immune response?
a. Phagocytosis
b. Production of antibody or complement
c. Induction of immunologic memory
d. Accelerated immune response upon subsequent exposure to antigen - ANSWER
a. Phagocytosis
Which cells are involved in the production of antibodies?
a. Dendritic cells
b. T lymphocytes
c. B lymphocytes
d. Macrophages - ANSWER c. B lymphocytes
Which of the following cells is involved in antigen recognition following phagocytosis?
a. B lymphocytes
b. T lymphocytes
c. Macrophages
d. Granulocytes - ANSWER b. T lymphocytes
The role of the macrophage during an antibody response is to:
a. Make antibody
b. Lyse virus-infected target cells
c. Activate cytotoxic T cells
d. Process antigen and present it - ANSWER d. Process antigen and present it
Which of the following immunoglobulins is produced in the primary immune
response?
a. IgA
b. IgE
c. IgG
d. IgM - ANSWER d. IgM
