C(ASCP) TEST METHODS &
PROCEDURES WITH ANSWERS
fluorometry ANSWER > gives more accurate (significantly more
sensitive & specific) concentration of whole DNA than
spectrophotometry or PCR
downsides:
= specific dyes used aren't stable for long periods & mixed
nucleic acid samples are difficult to quantify properly
capillary electrophoresis ANSWER = used in DNA sequencing
and several other molecular diagnostic procedures to separate
amplified products
> serves as an alternative testing method for HPLC
> used to separate organic chemicals and inorganic ions
serum protein electrophoresis ANSWER > property most
responsible for the migration of proteins in an electrical field is
'net surface charge'
> normal pattern has 5 distinct bands
>
, electrophoretic mobility (anodal to cathodal side) ANSWER
albumin
alpha 1
alpha 2
beta
gamma
chronic inflammation on SPE ANSWER => broad-based
increase in the gamma band, corresponding to polyclonal
hypergammaglobulinemia seen in this condition
> an increase in the alpha-1 & alpha-2 regions is also seen
multiple myeloma on SPE ANSWER => a tall narrow peak in
the beta region with decreased immunoglobulins in the gamma
region
> relatively fast migrating monoclonal protein present
polyacrylamide gel ANSWER used for electrophoresis
> has the greatest resolution or separation of solutes b/c of
varying pore size in each gel layer
12% acrylamide gel ANSWER most ideal for separating out
proteins about 80-120 bp in length
pulse-field electrophoresis ANSWER used for large DNA
methyl mercuric hydroxide ANSWER a buffer additive used in
gel electrophoresis to prevent complementary base pairing on
RNA molecules by interacting with the amino groups
parallel chemistry analyzer ANSWER all specimens analyzed
at the same time
PROCEDURES WITH ANSWERS
fluorometry ANSWER > gives more accurate (significantly more
sensitive & specific) concentration of whole DNA than
spectrophotometry or PCR
downsides:
= specific dyes used aren't stable for long periods & mixed
nucleic acid samples are difficult to quantify properly
capillary electrophoresis ANSWER = used in DNA sequencing
and several other molecular diagnostic procedures to separate
amplified products
> serves as an alternative testing method for HPLC
> used to separate organic chemicals and inorganic ions
serum protein electrophoresis ANSWER > property most
responsible for the migration of proteins in an electrical field is
'net surface charge'
> normal pattern has 5 distinct bands
>
, electrophoretic mobility (anodal to cathodal side) ANSWER
albumin
alpha 1
alpha 2
beta
gamma
chronic inflammation on SPE ANSWER => broad-based
increase in the gamma band, corresponding to polyclonal
hypergammaglobulinemia seen in this condition
> an increase in the alpha-1 & alpha-2 regions is also seen
multiple myeloma on SPE ANSWER => a tall narrow peak in
the beta region with decreased immunoglobulins in the gamma
region
> relatively fast migrating monoclonal protein present
polyacrylamide gel ANSWER used for electrophoresis
> has the greatest resolution or separation of solutes b/c of
varying pore size in each gel layer
12% acrylamide gel ANSWER most ideal for separating out
proteins about 80-120 bp in length
pulse-field electrophoresis ANSWER used for large DNA
methyl mercuric hydroxide ANSWER a buffer additive used in
gel electrophoresis to prevent complementary base pairing on
RNA molecules by interacting with the amino groups
parallel chemistry analyzer ANSWER all specimens analyzed
at the same time
