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DNA Methylation Methods and Protocols Second Edition

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Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii PART I. INTRODUCTION 1. DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker J¨org Tost. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 PART II. GLOBAL METHYLATION LEVELS 2. Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry Mar´ıa Berdasco, Mario F. Fraga, and Manel Esteller . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 3. Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels Kenneth P. Nephew, Curt Balch, and David G. Skalnik . . . . . . . . . . . . . . . . . . . . . . . . . 35 PART III. METHODS FOR WHOLE GENOME ANALYSIS OF DNA METHYLATION PATTERNS 4. Immunodetection Array Johannes Pr¨oll, Christian Wechselberger, Mathilde F¨odermayr, Otto Zach, and Dieter Lutz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 5. Methylated DNA Immunoprecipitation (MeDIP) Fabio Mohn, Michael Weber, Dirk Sch¨ubeler, and Tim-Christoph Roloff . . . . . . . . . . 55 6. The MIRA Method for DNA Methylation Analysis Tibor A. Rauch and Gerd P. Pfeifer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 7. The HELP Assay Mayumi Oda and John M. Greally . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 8. Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray Pearlly S. Yan, Dustin Potter, Daniel E. Deatherage, Shili Lin, and Tim H.-M. Huang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 9. Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS) Mireia Jord`a, Jairo Rodriguez, Jordi Frigola, and Miguel A. Peinado . . . . . . . . . . . 107 10. Methylation-Sensitive Representational Difference Analysis (MS-RDA) Toshikazu Ushijima and Satoshi Yamashita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

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Springer Protocols
Methods in Molecular Biology 507




DNA Methylation
Methods and Protocols
Second Edition


Edited by

Jörg Tost

,DNA Methylation

, M E T H O D S I N M O L E C U L A R B I O L O G Y TM
John M. Walker, SERIES EDITOR
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, TM
METHODS IN MOLECULAR BIOLOGY




DNA Methylation
Methods and Protocols

Second Edition


Edited by

Jörg Tost, PhD
CEA - Institut de Génomique, Evry, France

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Subido en
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Escrito en
2023/2024
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