Approach, 2nd ed. by Cowan
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Microbiology Fundamentals: Clinical Approach,
2nd ed. by Cowan Chapter2
Bacteria Correct Answer- Category of prokaryotes with peptidoglycan in their cell
walls and circular chromosome(s). This group of small cells is widely distributed in
the earth's habitats
Colony Correct Answer- A macroscopic cluster of cells appearing on a solid
medium, each arising from the multiplication of a single cell.
Culture Correct Answer- The visible accumulation of microorganisms in or on a
nutrient medium. Also, the propagation of microorganisms with various media.
Differential Media Correct Answer- A single substrate that discriminates between
groups of microorganisms on the basis of differences in their appearance due to
different chemical reactions.
Differential Stain Correct Answer- A technique that utilizes two dyes to distinguish
between different microbial groups or cell parts by color reactions.
Enriched Medium Correct Answer- A nutrient medium supplemented with blood,
serum, or some growth factor to promote the multiplication of fastidious
microorganisms.
Fastidious Correct Answer- Requiring special nutritional or environmental
conditions for growth; said of bacteria.
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Inoculation Correct Answer- The implantation of microorganisms into or upon
culture media.
Methods for Isolating Correct Answer- Based on the concept that if an individual
bacterial cell is separated from other cells and provided adequate space on a
nutrient surface, it will grow into a discrete mound of cells called a colony.
Requires a small number of cells be inoculated into a relatively large volume or
over a large area of medium.
Negative Stain Correct Answer- A staining technique that renders the background
opaque or colored and leaves the object unstained so that it is outlined as a
colorless area.
Positive Stain Correct Answer- A method for coloring microbial specimens that
involves a chemical that sticks to the specimen to give it color.
Resolving Power Correct Answer- The capacity of a microscope lens system to
accurately distinguish between two separate entities that lie close to each other.
Selective Media Correct Answer- Nutrient media designed to favor the growth of
certain microbes and to inhibit undesirable competitors.
Microbiology Correct Answer- Specialized area of biology that deals with living
things too small to be seen without magnification
Major groups of microorganisms in order from largest to smallest Correct Answer-
Bacteria
Archaea
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Protozoa
Fungi
Helminths
Viruses
Algae
Microbes are easy to study because... Correct Answer- They reproduce rapidly
They can be grown in large populations in the lab
Microbes are difficult to study because... Correct Answer- They can't be directly
seen & have to be analyzed through indirect means
Eu-kary Correct Answer- True nucleus
Pro-kary Correct Answer- Pre nucleus
Prokaryotes include... Correct Answer- Bacteria
Archaea
Prokaryotes have been on the planet... Correct Answer- 3.5 billion years
Eukaryotes have been on the planet... Correct Answer- 1.8 billion years
Eukaryotes were precursors to... Correct Answer- Organisms that eventually
formed multicellular animals
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