BACE Review Videos 5-8 Lab Procedures

How many milliliters does a BLUE serological pipette hold? - 1-2 mL How many milliliters does a GREEN serological pipette hold? - 5-10 mL How many milliliters does a RED serological pipette hold? - 25 mL How do you put together a serological pipette? - Press the tip into the pump How do you draw up liquid with a serological pipette? - Use the wheel to draw up liquid to the needed amount marked on the tip How should one check the accuracy of pipetting with a serological pipette? - Dispense into container on a balance How does one remove the tip from the pump on a serological pipette? - Click the button on the side Where should the buffer in a PCR buffer chamber come up to? - Just above the gel What is used to transfer the sample into the gel wells for PCR? - A micropipette How is dispensing a sample into PCR gel wells different than usual pipetting? - You should not press the plunger down to the second stop How should the lid be placed on a PCR chamber? - Red to red and black to black What settings should you adjust before running your gel during PCR? - Time and voltage or amps What button is pressed after truing on the PCR box to start the process? - The "run" button What PPE do you need before streaking plates? - Gloves What should you spray on you gloves and work surface before streaking your plate? - Isopropyl alcohol solution Where do you label a petri dish/plate? - The bottom perimeter What do you label a petri dish/plate with? - The date, your initials, and the type of sample streaked (ex.: 5/12/24, JH, e. coli) How should you open your plate to streak it? - Using the "seashell method" What tool is used to streak a sample onto the gel in a petri dish? - Inoculating loop What shape should you make when streaking on a gel? - a "z" or zig-zag Should you use a new inoculating loop for each new section on the petri dish/plate? - YES