ASCP MLS Exam Study Guide

ASCP MLS Exam Study Guide What feature is most often found with patients with a splenectomy on a Wright's stained blood smear? - Howell-Jolly bodies Patients with antibody to the following antigen are immune to Hepatitis B: - Surface antigen (HbSAg) Which two of the following tests are helpful for documenting previous Streptococcal throat and skin infections: - ASO titer and Anti-DNase B In mass spectrometry, the "appearance potential" is defined as the: - Minimum energy required for the appearance of a particular fragment ion in mass spectrum What is generally accepted as the lower threshold value for semen pH from fertile males? - 7.2 Coulometry: - An automated method for measuring chloride which generates silver ions in the reaction Erythroleukemia: - Also known as Acute Myelogenous Leukemia type M6. This type is associated with either the presence of both erythroid and myeloid precursors OR strictly erythroid precursors. Myelomonocytic leukemia - Is associated with an increase in myeloid and monocytic cell precursors, but not red blood cell precursors Species that are Lysine (+), arginine (=) and ornithine (+): - Enterobacter aerogenes, Edwardsiella species and most Serratia species Lysine reaction for all Citrobacter: - Negative Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - negative reactions for the three decarboxylases commonly tested ( Lysine, arginine and ornithine) Which organism is MOST likely responsible for septic reactions associated with Red Blood Cell transfusions? - Yersina entercolitica is most likely responsible for septic reactions in transfusions of Red Blood Cells. This organism is usually acquired by ingestion of contaminated food and causes mild symptoms of abdominal pain and diarrhea. Growth of Y. entercolitica is enhanced in iron-rich environments such as red cell components. Gaucher's disease - The most common lysosomal storage disease caused by insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the deposition of glucocerebroside in cells of the macrophage-monocyte system. Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) - Chediak- Higashi syndrome (CH), Chronic granulomatous disease (CGD), Myeloperoxidase deficiency (MPO) Nephrotic Syndrome: - Caused by damage to the kidneys, especially the basement membrane of the glomerulus; which causes abnormal excretion of protein and red blood cells in the urine. Fats are also present in the urine in most cases. A foamy appearance of the urine is a key characteristic of this condition. Clinical symptoms include: Swelling Weight gain from fluid retention Poor appetite High blood pressure Key Biochemical reactions for Enterobacteriaceae: - The key biochemical reactions by which the family Enterobacteriaceae can be identified include fermentation of carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase activity. ANA - See 2 quiz #27 Prozone effect: - Results in a false positive reaction; is the result of antibody excess and dilution of antibody can help prevent its occurrence Purpose of a bi-chromatic analyzer with dual wavelengths