Microbiology Chapter 9 Test Bank

In Figure 9.2, the enzyme in step 1 is A) DNA polymerase. B) DNA ligase. C) RNA polymerase. D) Reverse transcriptase. E) Spliceosome. C- RNA polymerase In Figure 9.2, the enzyme in step 2 is A) DNA polymerase. B) DNA ligase. C) RNA polymerase. D) Reverse transcriptase. E) Spliceosome. A- DNA polymerase The reaction catalyzed by reverse transcriptase is A) DNA → mRNA. B) mRNA → cDNA. C) mRNA → protein. D) DNA → DNA. E) tRNA → mRNA. C- mRNA - protein Which of the following is an advantage of using E. coli to make a human gene product? A) Endotoxin may be in the product. B) It does not secrete most proteins. C) Its genes are well known. D) It cannot process introns. E) Endotoxin may be in the product and it does not secrete most proteins. C- Its genes are well known If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is A) transformation of E. coli with Ti plasmid. B) Splicing tDNA into a plasmid. C) Transformation of an animal cell. D) Inserting the Ti plasmid into Agrobacterium. E) Inserting the Ti plasmid into a plant cell. D- Inserting the Ti plasmid into Agrobacterium Biotechnology involves the A) Use of microorganisms to make desired products. B) Use of animal cells to make vaccines. C) Development of disease-resistant crop plants. D) Use of microorganisms to make desired products and the use of animal cells to make vaccines. E) Use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease-resistant crop plants. D- Use the microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease-resistant crop plants Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures? A) Self-replication B) large size C) has a selectable marker D) circular form of DNA or integrates into the host chromosome E) may replicate in several species large size B-Large size