Histotechnology: Decalcification and Processing questions with 100% complete solutions

Histotechnology: Decalcification and Processing After completion of decalcification, the specimen should be - correct answers Washed in water Over decalcification is more noticeable in staining of nuclei or cytoplasm? - correct answers Nuclei Acid solutions soften bone by removing what type of salts? - correct answers Calcium salts What type of acid is a chelating agent used in decalcification? - correct answers EDTA (ethylene diamine tetra acetic acid) To offset the hydrolysis of nucleic acids caused by decalcification, bone marrow is often fixed in what solution? - correct answers Zenker's During decalcification, what type of gas is released? - correct answers Carbon dioxide What four factors influence the rate of decalcification? - correct answers Size of specimen, concentration of decal solution, Time in decal solution Amount of decal solution For proper decalcification, bone should be cut at no more than - correct answers 4-5 mm thick Chelating agents act by binding to: - correct answers Calcium ions Four methods of decalcification are: - correct answers Acid, Ion exchange, Electrolytic method, Chelation A clearing agent must be miscible with: - correct answers Dehydrants and infiltrating media In order, the three steps of processing are: - correct answers Dehydration, clearing and infiltration Two purposes of the clearing step are to: - correct answers Remove alcohol and make tissue receptive to the infiltration agent. Universal solvents can perform what two function simultaneously? - correct answers Dehydration and clearing. Name two universal solvents - correct answers Tertiary butanol, tetrahydrofuran Most universal solvents are suitable for use with delicate tissue T/F? - correct answers False Why are graded alcohols used for dehydration? - correct answers Gradually increasing concentration of the dehydrant reduces tissue shrinkage. If the clearing agent is cloudy after the tissue has been processed, it was probably contaminated with - correct answers Water Paraffin processing is not recommended for the preservation of - correct answers Enzymes Universal fixatives are unsuitable for a gastric biopsy T/F? - correct answers True Hydrolysis of tissue resulting in loss of basophilia results from - correct answers Overdecalcification In what organ is there an abrupt change in the type of epithelial cell found on its surface? - correct answers Cervix the organ which contains large cells with large central nuclei, and which secretes bile as one of its many functions is the... - correct answers Liver Which of the following infiltrating media is routinely used in electron microscopy? - correct answers Resin Ethanol is unavailable to use as a dehydrant. What is a good substitute to use? - correct answers Isopropanol