BACE Exam-Knowledge (A+ Guaranteed)

Clinical Trial- Phase 1 correct answers - Tests drug in healthy individuals - Tests for safety, dosage, and side effects Clinical Trial- Phase 2 correct answers - Tests on larger group of effected individuals - Tests for efficacy and side effects Clinical Trial-Phase 3 correct answers - Tests on new and wider demographic - Tests for long term effectiveness and comparisons with other medications Clinical Trial- FDA Approval correct answers Treatment determined effective and safe for public use Clinical Trial- Phase 4 correct answers - Continues to test for effectiveness and safety - Can be taken off the market if necessary Cell Harvesting- Indirect Cell Count correct answers Determine concentration of cells is a liquid culture DOES NOT tell me if the cells are viable vs nonviable- some cells may be dead Cell Harvesting- Indirect Cell Count Reagents correct answers - Luria Broth(Food Source) - Shaking Incubator or shaking water bath (Incubator or Water is for 37 C; Shaking-Oxygen) - Spectrophotometer (measures absorbance values to give concentration) Cell Harvesting- Direct Cell Count correct answers Determine concentration of cells in agar place Cell Harvesting- Direct Cell Count Reagents correct answers - Agar Plate - Luria Broth(food source) - Incubator (37 C) - Physically Count- the amount the colonies and calculate the concentration Gram Staining correct answers Determine if peptidoglycan is present in cell wall Gram Staining Reagents correct answers - Crystal Violet=Gram Positive - Iodine= fixation - Alcohol= decolorizer - Safranin= Gram negative Inserting into the Cell correct answers Neutralize cell membrane and substance being inserted now passive disuse can occur Inserting into the Cell- Reagents correct answers - Cofactors: CaCl2 or MgCl2 - Heat-shock (chemical transformation= prokaryotic) or Electricity/Electroporation (Transfection= Eukaryotic Cells)