Unit 17 AB
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Microbiology and Microbiological Techniques:
P3: Structures of microorganisms observed using a light microscope.
ELECTRON MICROGRAPHS OF MICROORGANISMS
Actual size = Size of the image / Magnification
Scanning electron microscope images:
1) E.Coli Magnification = 16,000 X
1
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Size of image: 5cm = 50000µm
Ans: 50000/16000 = 3.13µm
2) Staphylococcus aureus: Magnification:10,000X
Size of image: 1cm = 10000µm
Ans: 10000/10000 = 1µm
2
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Transmission electron microscope
1) Bacillus subtilis: Magnification: 16,000 x
Size of image: 2cm = 20000µm
Ans: 20000/16000 = 1.25µm
2) Diplococcus: Magnification:20,000 X
Size of image: 6cm = 60000µm
Ans: 60000/20000 = 3µm
3
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
SEM: Fungi:Yeast (Saccromyces Cervisicae) – Mag—7500 X
Size of image: 4cm = 40000µm
Ans: 40000/7500 = 5.32µm
TEM: Yeast Magnification: 12,000X
Size of image: 12cm = 120000µm
Ans: 120000/12000 = 10µm
4
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Virus T4 Bacteriophage– SEM - Magnification = 12,000X
Size of image: 2cm = 20000µm
Ans: 20000/12000 = 1.67µm
TEM: Magnification: 18,000X
Size of image: 10cm =
100000µm
5
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Ans:100000/18000 = 5.56µm
Protozoa: Amoeba
SEM: Magnification: 10,000 X
Size of image: 8cm = 80000µm
Ans: 80000/10000 = 8µm
6
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
TEM: Magnification: 16,500 X
Size of image: 11cm = 110000µm
Ans: 110000/16500 = 6.67µm
Paramecium:
SEM: Magnification: 8500x
Size of image: 8cm = 80000µm
Ans: 80000/8500 = 9.41
7
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
TEM: Magnification:12,500 X
Size of image: 13cm = 130000µm
Ans: 130000/12500 = 10.40µm
P2: Setting up and use of a light microscope to observe structures of microorganisms
under magnification.
Handling the Microscope
• Pick up and carry the microscope with two hands
• One hand placed under the base and the other firmly grasping the arm
• Place the microscope in the centre of the desk
• When using the microscope move yourself to the microscope don’t move the
microscope to you
• Plug in the microscope so that the chord is out of the way as much as possible
Handling Slides
• Grasp the slide by the edges so you do not place fingerprints on the top or bottom of
the slide
8
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written by
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Microbiology and Microbiological Techniques:
P3: Structures of microorganisms observed using a light microscope.
ELECTRON MICROGRAPHS OF MICROORGANISMS
Actual size = Size of the image / Magnification
Scanning electron microscope images:
1) E.Coli Magnification = 16,000 X
1
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Size of image: 5cm = 50000µm
Ans: 50000/16000 = 3.13µm
2) Staphylococcus aureus: Magnification:10,000X
Size of image: 1cm = 10000µm
Ans: 10000/10000 = 1µm
2
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Transmission electron microscope
1) Bacillus subtilis: Magnification: 16,000 x
Size of image: 2cm = 20000µm
Ans: 20000/16000 = 1.25µm
2) Diplococcus: Magnification:20,000 X
Size of image: 6cm = 60000µm
Ans: 60000/20000 = 3µm
3
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
SEM: Fungi:Yeast (Saccromyces Cervisicae) – Mag—7500 X
Size of image: 4cm = 40000µm
Ans: 40000/7500 = 5.32µm
TEM: Yeast Magnification: 12,000X
Size of image: 12cm = 120000µm
Ans: 120000/12000 = 10µm
4
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Virus T4 Bacteriophage– SEM - Magnification = 12,000X
Size of image: 2cm = 20000µm
Ans: 20000/12000 = 1.67µm
TEM: Magnification: 18,000X
Size of image: 10cm =
100000µm
5
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
Ans:100000/18000 = 5.56µm
Protozoa: Amoeba
SEM: Magnification: 10,000 X
Size of image: 8cm = 80000µm
Ans: 80000/10000 = 8µm
6
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
TEM: Magnification: 16,500 X
Size of image: 11cm = 110000µm
Ans: 110000/16500 = 6.67µm
Paramecium:
SEM: Magnification: 8500x
Size of image: 8cm = 80000µm
Ans: 80000/8500 = 9.41
7
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Najah Bendriss BTEC Applied Science Biology Unit 17AB
TEM: Magnification:12,500 X
Size of image: 13cm = 130000µm
Ans: 130000/12500 = 10.40µm
P2: Setting up and use of a light microscope to observe structures of microorganisms
under magnification.
Handling the Microscope
• Pick up and carry the microscope with two hands
• One hand placed under the base and the other firmly grasping the arm
• Place the microscope in the centre of the desk
• When using the microscope move yourself to the microscope don’t move the
microscope to you
• Plug in the microscope so that the chord is out of the way as much as possible
Handling Slides
• Grasp the slide by the edges so you do not place fingerprints on the top or bottom of
the slide
8
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