Testing for Macronutrients
Macronutrient Test Result
Starch Iodine test Colour change from brown
to blue
Protein Biuret test Colour change from blue to
purple
Lipids Ethanol test White milky sheet layer on
the top of the water
Reducing Sugars Benedict test Colour change from blue to
brick red
Non-Reducing Sugars Benedict test 1st test no colour change
2nd test colour change from
blue to brick red.
Vitamin C DCpip Colourless from Blue
Method: (Cheerios)
1. I inserted a small amount of cheerios into separate mortars and crush them into a
liquid solution using distilled water.
2. I then filtered the cheerios liquid away from the excess food using a filter and poured
this into a test tube.
3. After this I used a pipette to take a small amount of the cheerios liquid from the test
tube and put a small amount into 6 different test tubes creating a 1:1 ratio amount
with each macronutrient. (Iodine, Biuret, Ethanol, Benedicts x2 and DCPIP)
4. Lastly I put the test tube with both mixes into a water bath to help quicken the
reaction while I waited for them to change colour.
5. I wrote down all the results on a table.
Method: (Potato)
1. I inserted a small amount of potatoes into separate mortars and crush them into a
liquid solution using distilled water.
2. I then filtered the potato liquid away from the excess food using a filter and poured
this into 5 different test tubes for each macronutrient.
3. After this I used a pipette to take a small amount of the potato liquid from the test
tube and put a small amount into 6 different test tubes creating a 1:1 ratio amount
with each macronutrient. (Iodine, Biuret, Ethanol, Benedicts x2 and DCPIP)
4. Lastly I put the test tube with both mixes into a water bath to help quicken the
reaction while I waited for them to change colour.
5. I wrote down all the results on a table.
8
Macronutrient Test Result
Starch Iodine test Colour change from brown
to blue
Protein Biuret test Colour change from blue to
purple
Lipids Ethanol test White milky sheet layer on
the top of the water
Reducing Sugars Benedict test Colour change from blue to
brick red
Non-Reducing Sugars Benedict test 1st test no colour change
2nd test colour change from
blue to brick red.
Vitamin C DCpip Colourless from Blue
Method: (Cheerios)
1. I inserted a small amount of cheerios into separate mortars and crush them into a
liquid solution using distilled water.
2. I then filtered the cheerios liquid away from the excess food using a filter and poured
this into a test tube.
3. After this I used a pipette to take a small amount of the cheerios liquid from the test
tube and put a small amount into 6 different test tubes creating a 1:1 ratio amount
with each macronutrient. (Iodine, Biuret, Ethanol, Benedicts x2 and DCPIP)
4. Lastly I put the test tube with both mixes into a water bath to help quicken the
reaction while I waited for them to change colour.
5. I wrote down all the results on a table.
Method: (Potato)
1. I inserted a small amount of potatoes into separate mortars and crush them into a
liquid solution using distilled water.
2. I then filtered the potato liquid away from the excess food using a filter and poured
this into 5 different test tubes for each macronutrient.
3. After this I used a pipette to take a small amount of the potato liquid from the test
tube and put a small amount into 6 different test tubes creating a 1:1 ratio amount
with each macronutrient. (Iodine, Biuret, Ethanol, Benedicts x2 and DCPIP)
4. Lastly I put the test tube with both mixes into a water bath to help quicken the
reaction while I waited for them to change colour.
5. I wrote down all the results on a table.
8
