ASCP Exam |173 Questions| with complete solutions

Which is required to generate cDNA A. DNA polymerase B. RNase H C. Restriction Enzyme D. Helicase Correct Answer: B What is the function of Taq Polymerase Correct Answer: recognize dsDNA You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How much stock should you add(what is the dilution factor) Correct Answer: 2 What gene causes hypersensitivity to Irinotecan? Correct Answer: UGT1A Which allele causes hypersensitivity to Carbamazepine? Correct Answer: HLA-b*1502 What gives MLPA its specificity Correct Answer: Stuffer Sequence How is copy number determined? Correct Answer: MLPA What is the gene that cause resistance to Vancomycin Correct Answer: vanA Which assay is FISH best used for Correct Answer: Prader Willi RNA polymerase II: where is it and what does it do Correct Answer: Eukaryotic: It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA. (transcribes mRNA) Hazard that is blue and is level 4 what does it mean Correct Answer: big health hazard Dr calls wanting test results but results have not been signed out by a medical director what do you do Correct Answer: don't give out the result until it has been signed out my the MD What trinucleotide repeat is involved in Fragile X? Correct Answer: CGG In Fragile X, what is considered a full mutation expansion? Correct Answer: 200+ What method is used to detect Fragile X? Correct Answer: Southern blot (now PCR /mPCR) Which gene is the largest and has deletions/duplications as the most common mutation types Correct Answer: DMD