MLT Urinalysis questions with complete solutions

pH Reaction Correct Answer: Double indicator Methyl Red and Bromethymol Blue Protein Reaction Correct Answer: Protein error of indicator Glucose Reaction Correct Answer: Glucose Oxidase Ketones Reaction Correct Answer: sodium nitroprusside Blood Reaction Correct Answer: peroxidase activity of Heme Myoglobin Reaction Correct Answer: Ammonium sulfate screen centrifuge sample and then test with Blood reagent strip positive = Myoglobin Bilirubin Reaction Correct Answer: Diazo reaction Diazonium salt > azobilirubin Urobilinogen Reaction Correct Answer: Para-dimethyl Elrich's Nitrite Reaction Correct Answer: Greiss Reaction Diazotization of nitrite w/ aromatic amino > Diazonium salt Leuocyte Reaction Correct Answer: Ester and Diazonium salt Ester + aromatic compound > Diazonium salt pH erroneous results Correct Answer: improper storage (increase) contaminated container Improper technique > leaching buffer Protein false positives Correct Answer: Highly buffered alkaline urine Allowing pad to stay in contact with urine too long > may leach out buffer Contamination of container with ammonium compounds or detergents > alkaline cleaning agents Highly colored substances, eg pyridium, beets (mask color) Protein false negatives Correct Answer: Globulin Myoglobin Bence Jones protein Highly colored substances, eg pyridium, beets (mask color)