intro to cryo-electron microscopy
is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures and embedded in an environment of vitreous water. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane.[2] While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.[3] This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.
Escuela, estudio y materia
- Institución
- Stanford University
- Grado
- Cryo-EM
Información del documento
- Subido en
- 22 de febrero de 2022
- Número de páginas
- 69
- Escrito en
- 2020/2021
- Tipo
- Notas de lectura
- Profesor(es)
- Ron dror
- Contiene
- Todas las clases
Temas
-
electron microscopy
-
cryo em
-
cryogenic electron microscopy