BSC 3403C LAB REPORT 1.
, Latocha 1
Logan Latocha
Prof. Verity
BSC 3403C
1/23/22
Lab Report 1
Principle of Methods: The purpose or objective of this lab is to familiarize ourselves with
basic lab equipment that we will be using for the rest of the semester in lab. We will be
understanding how to accurately use and safely operate things such as a Micropipette, centrifuge,
and spectrophotometer. The experiment begins by taking a large-sized micropipette (enough to
hold 800 µl), A micropipette is a transfer device used to transfer very small amount of liquids,
hence the name micro. There is a different range set for each pipette used for different scenarios,
each with its corresponding tip to hold the liquid. The amount of liquid dispensed is based on the
number on the micropipette, this can be adjusted by turning the knob just below the plunger for
both Denville and Eppendorf. The pipette should be held firmly with all fingers wrapped around
the pipette with the thumb on the plunger. Now to aspirate or take up liquid in your pipette with
the corresponding tip on tightly, first simply press on the plunger until a small bit of resistance is
felt, this is the first stop (we do not go to the second stop because this will aspirate more than the
amount shown on the outside of the micropipette). Once that is done slowly move the pipette
down and into the desired liquid until the tip is barely submerged (about 0.5 an inch) then slowly
release the plunger and the liquid will be aspirated. For dispensing everything is the same but
there are a few minor tweaks, first lower the pipette tip close to the liquid you are dispensing into
but not in the liquid because this will cause cross-contamination. Then instead of releasing the
plunger, the plunger is pressed down first to the first stop and then to the second stop to ensure
that all of the liquid is out. After this is done eject the pipette tip into waste, which is done by
pressing the button below and to the side of the plunger. This process is done with the
This study source was downloaded by 100000817936687 from CourseHero.com on 02-19-2022 09:55:46 GMT -06:00
https://www.coursehero.com/file/128524983/Lab-Report-1docx/
, Latocha 1
Logan Latocha
Prof. Verity
BSC 3403C
1/23/22
Lab Report 1
Principle of Methods: The purpose or objective of this lab is to familiarize ourselves with
basic lab equipment that we will be using for the rest of the semester in lab. We will be
understanding how to accurately use and safely operate things such as a Micropipette, centrifuge,
and spectrophotometer. The experiment begins by taking a large-sized micropipette (enough to
hold 800 µl), A micropipette is a transfer device used to transfer very small amount of liquids,
hence the name micro. There is a different range set for each pipette used for different scenarios,
each with its corresponding tip to hold the liquid. The amount of liquid dispensed is based on the
number on the micropipette, this can be adjusted by turning the knob just below the plunger for
both Denville and Eppendorf. The pipette should be held firmly with all fingers wrapped around
the pipette with the thumb on the plunger. Now to aspirate or take up liquid in your pipette with
the corresponding tip on tightly, first simply press on the plunger until a small bit of resistance is
felt, this is the first stop (we do not go to the second stop because this will aspirate more than the
amount shown on the outside of the micropipette). Once that is done slowly move the pipette
down and into the desired liquid until the tip is barely submerged (about 0.5 an inch) then slowly
release the plunger and the liquid will be aspirated. For dispensing everything is the same but
there are a few minor tweaks, first lower the pipette tip close to the liquid you are dispensing into
but not in the liquid because this will cause cross-contamination. Then instead of releasing the
plunger, the plunger is pressed down first to the first stop and then to the second stop to ensure
that all of the liquid is out. After this is done eject the pipette tip into waste, which is done by
pressing the button below and to the side of the plunger. This process is done with the
This study source was downloaded by 100000817936687 from CourseHero.com on 02-19-2022 09:55:46 GMT -06:00
https://www.coursehero.com/file/128524983/Lab-Report-1docx/
