BACTERIOLCCY
bauteria
important Whyis theStndyof
abundunce
,diversity andqibai distribution
bacttriantilizesCarbonandenerqy souvces
path waysresponsiblefor recyuling
metabolic
diversity
chemotrophs
chemoorgandtrophs
nse
organic aselectron
componnds
chemolithotrophs |
donors
nse
range aerobically oranaerobitaly
of inorganic Substances
photot rophs
ph otoheterotrophy
photo autotro
com nseUsephotosynthesis
organic pounds
phy
nseC02
baiteriacan
inhabitextreme
environment
psychvophile
e
temperatuy ID0
mesophile
moderate
temperature
lopt)
E.coli
ther mophile
temperature
high
evolutionlife of archaea
bacteria enkayya
carty life was
andxygenic
evidence ofceils
forearlyemergence
nthin
primordial soup
hydyocoybons
world RNA
lipid vesicies
fivstcenlmiar
life tree LUCA
3 domains
Stromatolifes
enkavvotic
origins
bacteria ofarchae
fusion and bauteria archaca
Archae bacteria
avchaeon ct-protebbatterium
endosymbiotic
nucleus mitochondria cocyte
enkaryotes >to
tyee relate
bacteriaarcheavs
bothare
prokaryotes
ave
in archaeadifferent
and
ceilmembranesceilwall incen
nopeptidoglycan,branched
hydvocarbons membrane
nnatfelted chibrampheniiol
byStreptomytin/
Vibosomal
antibiotius
reters
dogma
Centyal tDenkayyotes
©olivia_liying
,heaith anddisease
more baiteria than human
ceils
bacterial infeitions
iFluenza
streptococens (most
staphylocoens anrens
p
common) neu
infinenza
nsduringSeasonal
co-infectio
monine
Avonp A
Streptococeus infettion bacterial
secndavy
T6
septilaemia : balteria in multiplyingbloo
d
organ failnre
antibiotic yesistance
hiStoryof
micyobiblogy
firs t goidenage
of themiuroscopeinvention
asngents of infeutionsdisease
micvobes
second
goiden age
antibrotic
bibloqy andmolecmiar
LowisPastenr
infermentation
roleofmicroorganism
disproving
vallines
Spontaneous
Permentation
genevation
thesonyce
0f
as
RobertKoch
Microbiologial methods
plateslnitures AQaY
pureispiation of cmtuye
discoveryofcansutive diseases
infeutions
agents 6f
postuiatesKoch's aputhogenwithdiseasethe
Connelt
hen
principles to
reqnived
thepenicilimstovy Norman chain,Ernst
Heatley
ward
Florey Alexander Ho
Fleming,
food
were
andbrotechnoiogy
mast
discoveries
DNA
made
using E-coli
key
centraldoqma
code!
Genetic
genes
requiatory uacopern)
©olivia_liying
,appendages baterinl shape And
stainingtechniques and
lignt microscory
prepavation
Fixation
is killedandattalhed anism
ora to slide
heatfixrng
morpholoqu overallnotinternalstrnitnres
preserves
staining
increases
visibility
-
simple Staining
staindireatly>crustal
charges rinletdues
withpositive
ceu
wallcavries
negativecharge
stain qvamthe
aram aram
•
fixotion
cvustalvidlet
todine treatment
decolorization
counter stain
(safranin)
purpie pink
( Staphyiococysaurems) E.coli)
shapesof bacteria
00
couus dipidcoui
pr
.•..• streptocouuscorunebauteria
sarcina staphylowuus
ae
batillus Spirilla sprrochete VibYiO
©olivia_liying
, deveiopmentof milyoslopy
electronmicroscopy
artefalts mann
single nuciea nD
compartment
envelope
impressronof simplicity
:
cryoelectron milroscopy t tomography
-
Avoidsavtefauts
in lvestate
flashfrozen
sampies
3D
ContrastPooY
diqital imagíng finorescence
microscopy +
withfinorescentdue cellcomponents
Specifi
immunoflnorescence
visnairsed
green
flnorescent taqs
proteinCGFP)
-
micros limit) light
Copu(^200nm
superresoiution
Micxoscopy fluorescence
2ximprovementin resolution
immunotinorescence green finovestent
proteinGFf
tagging
componentdetect
Specific cenl jelytish from
antibody with
flnorestent make to
geneticmanipuiation
protein:detelt
native,wildtupe protein GFP a
fusion
andbefixedcens
permeabilised to
need imaging Irve ceu,timelapse
antibody access
AILDU0to colouY variants :
different proteins
Visnalization
of
timelapseimaging(fixed) nD proteinfusion 2
maifunution
mistendinglocalisation
manbe
strnuturesbalteria of
wall cell
-
peptidoalycan +vom
made
present andín batteria
LUCA
proteltionfromdamage and
lysis
Antibioticstargetof
sustem byinnate
recognised immune 03dta15
gramt cen
wau qrAm cell wall
Irpoteichoic
0OC g3o-cousau
8porin core
LPS
04005 7 polysauhande
Acid
OuteY lípia A
tcichoicaud
membrane
RERRIERTIRARO
peptidoglycan ipoprotein
bdo8os8o&dg
peptidoglycan pexiplasm
TH 401.peptidoglylan
telchoic.lipoterchoi aUid
cenmembrane
J07-peptidogiycan
bilayeronter
membrance
Selond
lipid :
lipoporysacchavde
LPS
endotoxin
complexliprd A
©olivia_liying
:
porin transport molecutes sman
altivity Site
perpiasm ofenzymic
bauteria
important Whyis theStndyof
abundunce
,diversity andqibai distribution
bacttriantilizesCarbonandenerqy souvces
path waysresponsiblefor recyuling
metabolic
diversity
chemotrophs
chemoorgandtrophs
nse
organic aselectron
componnds
chemolithotrophs |
donors
nse
range aerobically oranaerobitaly
of inorganic Substances
photot rophs
ph otoheterotrophy
photo autotro
com nseUsephotosynthesis
organic pounds
phy
nseC02
baiteriacan
inhabitextreme
environment
psychvophile
e
temperatuy ID0
mesophile
moderate
temperature
lopt)
E.coli
ther mophile
temperature
high
evolutionlife of archaea
bacteria enkayya
carty life was
andxygenic
evidence ofceils
forearlyemergence
nthin
primordial soup
hydyocoybons
world RNA
lipid vesicies
fivstcenlmiar
life tree LUCA
3 domains
Stromatolifes
enkavvotic
origins
bacteria ofarchae
fusion and bauteria archaca
Archae bacteria
avchaeon ct-protebbatterium
endosymbiotic
nucleus mitochondria cocyte
enkaryotes >to
tyee relate
bacteriaarcheavs
bothare
prokaryotes
ave
in archaeadifferent
and
ceilmembranesceilwall incen
nopeptidoglycan,branched
hydvocarbons membrane
nnatfelted chibrampheniiol
byStreptomytin/
Vibosomal
antibiotius
reters
dogma
Centyal tDenkayyotes
©olivia_liying
,heaith anddisease
more baiteria than human
ceils
bacterial infeitions
iFluenza
streptococens (most
staphylocoens anrens
p
common) neu
infinenza
nsduringSeasonal
co-infectio
monine
Avonp A
Streptococeus infettion bacterial
secndavy
T6
septilaemia : balteria in multiplyingbloo
d
organ failnre
antibiotic yesistance
hiStoryof
micyobiblogy
firs t goidenage
of themiuroscopeinvention
asngents of infeutionsdisease
micvobes
second
goiden age
antibrotic
bibloqy andmolecmiar
LowisPastenr
infermentation
roleofmicroorganism
disproving
vallines
Spontaneous
Permentation
genevation
thesonyce
0f
as
RobertKoch
Microbiologial methods
plateslnitures AQaY
pureispiation of cmtuye
discoveryofcansutive diseases
infeutions
agents 6f
postuiatesKoch's aputhogenwithdiseasethe
Connelt
hen
principles to
reqnived
thepenicilimstovy Norman chain,Ernst
Heatley
ward
Florey Alexander Ho
Fleming,
food
were
andbrotechnoiogy
mast
discoveries
DNA
made
using E-coli
key
centraldoqma
code!
Genetic
genes
requiatory uacopern)
©olivia_liying
,appendages baterinl shape And
stainingtechniques and
lignt microscory
prepavation
Fixation
is killedandattalhed anism
ora to slide
heatfixrng
morpholoqu overallnotinternalstrnitnres
preserves
staining
increases
visibility
-
simple Staining
staindireatly>crustal
charges rinletdues
withpositive
ceu
wallcavries
negativecharge
stain qvamthe
aram aram
•
fixotion
cvustalvidlet
todine treatment
decolorization
counter stain
(safranin)
purpie pink
( Staphyiococysaurems) E.coli)
shapesof bacteria
00
couus dipidcoui
pr
.•..• streptocouuscorunebauteria
sarcina staphylowuus
ae
batillus Spirilla sprrochete VibYiO
©olivia_liying
, deveiopmentof milyoslopy
electronmicroscopy
artefalts mann
single nuciea nD
compartment
envelope
impressronof simplicity
:
cryoelectron milroscopy t tomography
-
Avoidsavtefauts
in lvestate
flashfrozen
sampies
3D
ContrastPooY
diqital imagíng finorescence
microscopy +
withfinorescentdue cellcomponents
Specifi
immunoflnorescence
visnairsed
green
flnorescent taqs
proteinCGFP)
-
micros limit) light
Copu(^200nm
superresoiution
Micxoscopy fluorescence
2ximprovementin resolution
immunotinorescence green finovestent
proteinGFf
tagging
componentdetect
Specific cenl jelytish from
antibody with
flnorestent make to
geneticmanipuiation
protein:detelt
native,wildtupe protein GFP a
fusion
andbefixedcens
permeabilised to
need imaging Irve ceu,timelapse
antibody access
AILDU0to colouY variants :
different proteins
Visnalization
of
timelapseimaging(fixed) nD proteinfusion 2
maifunution
mistendinglocalisation
manbe
strnuturesbalteria of
wall cell
-
peptidoalycan +vom
made
present andín batteria
LUCA
proteltionfromdamage and
lysis
Antibioticstargetof
sustem byinnate
recognised immune 03dta15
gramt cen
wau qrAm cell wall
Irpoteichoic
0OC g3o-cousau
8porin core
LPS
04005 7 polysauhande
Acid
OuteY lípia A
tcichoicaud
membrane
RERRIERTIRARO
peptidoglycan ipoprotein
bdo8os8o&dg
peptidoglycan pexiplasm
TH 401.peptidoglylan
telchoic.lipoterchoi aUid
cenmembrane
J07-peptidogiycan
bilayeronter
membrance
Selond
lipid :
lipoporysacchavde
LPS
endotoxin
complexliprd A
©olivia_liying
:
porin transport molecutes sman
altivity Site
perpiasm ofenzymic
