ETHICS IN BIOTECHNOLOGICAL
PRACTICES CERTIFICATION TEST WITH
ACCURATE ANSWERS
◉How many cycles of PCR does it take to produce a double-stranded
DNA fragment of the desired length? Ans: 3
◉What is transformation? Ans: The insertion of a foreign plasmid into a
bacterial cell which results in new acquired traits.
◉PCR happens in 3 steps (denaturing, annealing, extending) or 1
"cycle". How many times are cycles typically repeated? Ans: 30
◉What is the purpose of gel electrophoresis? Ans: separates DNA based
on size
◉What is pGlo? Ans: A plasmid with genes for GFP and ampicillin
resistance
, ◉LB containing ampicillin (2nd plate) is inoculated with the E. coli
bacteria that contains ampicillin resistant gene. The bacteria will: Ans:
Grow on the agar plate
◉What did we use to create temperature differential to sweep plasmid
inside? Ans: heat shock
◉At what temperature does the Denature step of PCR occur? Ans: 94-
96° C
◉At what temperature does the Anneal step of PCR occur? Ans: 50-65°
C
◉You decide to use PCR to determine if you have Cystic Fibrosis, a
genetic disorder. You then decide to also check if you are at risk for
developing Alzheimers, another genetic disorder. What PCR
"ingredient" would be different in these two tests? Ans: Primers
◉What happens in the Denature step of PCR? Ans: The double-
stranded DNA is separated into two single strands of DNA.
◉Why does PCR require a specific type of polymerase? Ans: Taq
polymerase can withstand the range of temperatures in PCR without
denaturing.
PRACTICES CERTIFICATION TEST WITH
ACCURATE ANSWERS
◉How many cycles of PCR does it take to produce a double-stranded
DNA fragment of the desired length? Ans: 3
◉What is transformation? Ans: The insertion of a foreign plasmid into a
bacterial cell which results in new acquired traits.
◉PCR happens in 3 steps (denaturing, annealing, extending) or 1
"cycle". How many times are cycles typically repeated? Ans: 30
◉What is the purpose of gel electrophoresis? Ans: separates DNA based
on size
◉What is pGlo? Ans: A plasmid with genes for GFP and ampicillin
resistance
, ◉LB containing ampicillin (2nd plate) is inoculated with the E. coli
bacteria that contains ampicillin resistant gene. The bacteria will: Ans:
Grow on the agar plate
◉What did we use to create temperature differential to sweep plasmid
inside? Ans: heat shock
◉At what temperature does the Denature step of PCR occur? Ans: 94-
96° C
◉At what temperature does the Anneal step of PCR occur? Ans: 50-65°
C
◉You decide to use PCR to determine if you have Cystic Fibrosis, a
genetic disorder. You then decide to also check if you are at risk for
developing Alzheimers, another genetic disorder. What PCR
"ingredient" would be different in these two tests? Ans: Primers
◉What happens in the Denature step of PCR? Ans: The double-
stranded DNA is separated into two single strands of DNA.
◉Why does PCR require a specific type of polymerase? Ans: Taq
polymerase can withstand the range of temperatures in PCR without
denaturing.
