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Examen

BIO201 Lab 3 – Biology Laboratory Exercises and Practical Study Guide

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BIO201 Lab 3 – Biology Laboratory Exercises and Practical Study Guide

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Lab 3 Mitosis and Meiosis BIO201L

Student Name: Adaeze Efunobi
Access Code (located on the lid of your lab kit): AC-03FCGR


Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected. Pre-
lab Questions
1. What are chromosomes made of?
Chromosomes are composed of long strands of coiled DNA molecules.


2. Compare and contrast mitosis and meiosis, ensuring to detail the differences in both function
and in process. (Hint: Your response should be 4-5 sentences long.)
Mitosis and meiosis share similarities in stages such as interphase, prophase, metaphase,
anaphase, telophase, and cytokinesis. However, meiosis involves two rounds of cell division,
resulting in the production of four haploid gamete cells, while mitosis produces two identical
diploid cells. The key distinction lies in meiosis contributing to genetic diversity through crossing
over and independent assortment, crucial for sexual reproduction. In contrast, mitosis is
responsible for growth, tissue repair, and maintaining the organism's somatic cells.


3. Cancer is a disease related to uncontrolled cell division. Investigate two known causes for these
rapidly dividing cells and use this knowledge to invent a drug that would inhibit the growth of
cancer cells. (Hint: Your response should be 4-5 sentences long.)
Two known causes of uncontrolled cell division in cancer include mutations in genes that
accelerate cell division rates or inhibit normal cell cycle controls, such as mutations in the tumor
suppressor gene p53. To inhibit the growth of cancer cells, a potential drug could target the p53
pathway, mimicking its function to suppress uncontrolled cell division and prevent tumor
formation.




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Lab 3 Mitosis and Meiosis BIO201L

EXPERIMENT 1: OBSERVATION OF MITOSIS IN A PLANT CELL
Introduction Questions
1. State how you will calculate the number of hours each stage of mitosis takes based on
observations. Do so by either stating the “Hours of Stage” equation, or by discussing the
equation and its application. If you decide to insert an equation, you must use an equation
editor to properly state the equation with the correct symbols and fraction format.
To calculate the time spent in each stage of mitosis, I will use the "Hours of Stage" equation,
which involves measuring the proportion of cells in each stage and multiplying it by the total
duration of the cell cycle. The equation is as follows: Hours of Stage=(Number of Cells in
Stage/Total Number of Cells)×Total Time of Cell CycleHours of Stage=(Total Number of
CellsNumber of Cells in Stage)×Total Time of Cell Cycle


2. In Table 1, below, predict how many hours of the 24-hour cell cycle you think each step takes.
Ensure you provide robust supporting evidence to back your prediction.


Table 1: Mitosis Predictions

Interphase – 22 hours Prophase – 40 minutes Metaphase –
Predictions
Cytokinesis – 20 minutes
Interphase lasts anywhere from 18-24 hours while mitosis only
occupies 1 to 2 hours of the cell cycle. We predict that interphase will
Supporting Evidence last 22 hours, as supported above, with prophase being the longest of
the mitosis stages at 40 minutes and the rest of the stages taking
approximately 20 minutes totally out to be a 24 hour cell cycle.




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Lab 3 Mitosis and Meiosis BIO201L

Data and Observations
Record your observations in the table below. Based on those observations, calculate the percentage of
time spent in each phase of mitosis.

Table 2: Mitosis Data

Chosen Image Onion root tip, 100x - 4th (last) picture

Number of Cells in Calculated % of Time
Stage Total Number of Cells
Stage Spent in Stage

Interphase 18 25 72%

Prophase 2 25 8%

Metaphase 0 25 0%

Anaphase 1 25 4%

Telophase 2 25 8%

Cytokinesis 2 25 8%




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