BIOD 171 MODULE 3 EXAM SCRIPT
COMPLETE ANSWERS GRADED A+
◉ How do confocal microscopes work?
Answer: combine the usefulness of fluorescence microscopy with
the ability to visualize cells in 3D unlike light or fluorescence
microscopy where light is focused into a single 2D plane.
◉ How do stains work?
Answer: stains are salts, which break down into positively and
negatively charged ions
chromophore is the color in the dye
◉ acidic dyes
Answer: anionic (-) chromophores that stain positively charged
structures in acidic conditions mostly used for negative staining
◉ basic dyes
Answer: cationic (+) chromophores that stain negatively charged
structures in basic conditions (high pH), most common dye
◉ simple stain
Answer: simple stain is a single basic dye with and involve soaking
in the dye for 30-60 seconds and rinsing ex - crystal violet,
safranin, fuschin, or methylene blue
COMPLETE ANSWERS GRADED A+
◉ How do confocal microscopes work?
Answer: combine the usefulness of fluorescence microscopy with
the ability to visualize cells in 3D unlike light or fluorescence
microscopy where light is focused into a single 2D plane.
◉ How do stains work?
Answer: stains are salts, which break down into positively and
negatively charged ions
chromophore is the color in the dye
◉ acidic dyes
Answer: anionic (-) chromophores that stain positively charged
structures in acidic conditions mostly used for negative staining
◉ basic dyes
Answer: cationic (+) chromophores that stain negatively charged
structures in basic conditions (high pH), most common dye
◉ simple stain
Answer: simple stain is a single basic dye with and involve soaking
in the dye for 30-60 seconds and rinsing ex - crystal violet,
safranin, fuschin, or methylene blue
