MLT ASCP BOC Blood Bank EXAM LATEST VERSION
2026-2027 QUESTIONS AND ANSWERS
which of the following reagents is used to facilitate hem-agglutination following the
sensitization of red cells with an IgG alloantibody? - answer>>anti-human globulin serum
the addition of antibody-sensitized red cells (check cells) to all negative anti-human globulin
(AHG) tests ensures that:the test was interpreted correctly - answer>>AHG reagent was added
to each test
in which of the following clinical situations will the direct antiglobulin test be positive? -
answer>>hemolytic disease of the fetus and newborn
in which of the following is the indirect antiglobulin test utilized? - answer>>antibody detection
(screening) test
polyspecific AHG reagents - answer>>anti-IgG and anti-C3d
a false negative direct antiglobulin test can be the result of: - answer>>insufficient saline
washing of red cells
a false positive indirect antiglobulin test can be the result of: - answer>>overcentrifugation
a negative result using solid phase adherence assays will demonstrate indicator red cells as: -
answer>>a RBC pellet in the bottom of the well
a 4+ positive reactions using gel technology will appear as RBCs: - answer>>in a layer at the top
of the gel media
,one of the advantages of performing antibody screening (detection) studies using gel technology
is: - answer>>saline washing is not required
monoclonal blood banking reagents have which of the following as a disadvantage? -
answer>>overspecificity
the direct antiglobulin test in a suspected case of warm autoimmune hemolytic anemia is
positive. Which of the following monospecific reagents would be used in further direct
antiglobulin testing? - answer>>anti-C3d
low ionic strength saline (LISS) acts as an enhancement medium and facilitates antibody uptake
by: - answer>>reducing zeta potential
the membrane attack complex (MAC) formed during the activation of complement via the
classical pathway consists of: - answer>>C5b through C9
in ABO hemolytic transfusion reactions, complement is activated via which of the following
pathways? - answer>>classical
IgG-sensitized red cells that activate complement may not complete complement activation to
cell lysis. In addition to IgG on the surface of these red cells, what complement component is
present? - answer>>C3b
In a normal adult, the spleen acts as a site for: - answer>>removal of aging red blood cells
which of the following cells transport O2 and CO2? - answer>>erythrocytes
which of the following cells is the largest cell in the bone marrow? - answer>>megakaryocyte
in patients who present with bleeding due to a low platelet count, the most common type of
bleeding is: - answer>>mucosal bleeding
,Transfusion of which of the following is needed to help correct hypofibrinogenemia due to DIC?
- answer>>cryoprecipitated AHF
a blood component used in the treatment of hemophilia A is: - answer>>factor VIII
a unit of Fresh Frozen Plasma was inadvertently thawed and then immediately refrigerated at 4
oC on Monday morning. On Tuesday evening this unit may still be transfused as a replacement
for: - answer>>Factor IX
a newborn demonstrates petechiae, ecchymosis and mucosal bleeding. the preferred blood
component for this infant would be: - answer>>Platelets
which of the following would be the best source of platelets for transfusion in the case of
alloimmune neonatal thrombocytopenia? - answer>>mother
fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by which of the following
maternal antibodies? - answer>>IgG alloantibodies against HPA antigens
an obstetrical patient has had 3 previous pregnancies. Her first baby was healthy, the second
was jaundiced at birth and required an exchange transfusion, while the third was stillborn.
Which of the following is the most likely cause? - answer>>Rh incompatibility
ABO-hemolytic disease of the newborn: - answer>>is usually seen only in the newborn of group
O mothers
which of the following antigens is MOST likely to be involved in hemolytic disease of the fetus
and newborn? - answer>>Kell
ABO hemolytic disease of the fetus and newborn (HDFN) differs from Rh HDFN in that: -
answer>>Rh HDFN is clinically more severe than ABO HDFN
, a group A Rh positive infant of a group O, Rh positive mother has a weakly positive DAT and
moderately elevated bilirubin 12 hours after birth. the most likely cause is: - answer>>ABO
incompatability
in suspected cases of hemolytic disease of the fetus and newborn, what significant information
can be obtained from the baby's blood smear? - answer>>determination of the presence of
spherocytes
in treating hyperbilirubinemia in hemolytic disease of teh fetus and newborn (HDFN), light
phototherapy is used to: - answer>>breakdown unconjugated bilirubin to a nontoxic isomer
the purpose of performing antibody titers on serum from an immunized pregnant woman is to: -
answer>>identify candidates requiring additional fetal monitoring
which unit should be selected for exchange transfusion if the newborn is group A, Rh-positive
and the motehr is group A, Rh-positive with anti-c? - answer>>CDe/CDe
a mother is group A, with anti-D in her serum, what would be the preferred blood product if an
intrauterine transfusion is indicated? - answer>>Rh-negative RBC, Irradiated, CMV safe, HgbS-
negative
laboratory studies of maternal and cord blood yield the following results:
maternal blood= O,
Rh negative;
anti-E in serum
cord blood = B,
Rh positive, DAT=2+, anti-E in eluate - answer>>O, Rh-positive, E-
a blood specimen from a pregnant woman is found to be group B, Rh-negative and the serum
contains anti-D with a titer of 512. What would be the most appropriate type of blood to have
available for a possible exchange transfusion for her infant? - answer>>O, Rh-negative
2026-2027 QUESTIONS AND ANSWERS
which of the following reagents is used to facilitate hem-agglutination following the
sensitization of red cells with an IgG alloantibody? - answer>>anti-human globulin serum
the addition of antibody-sensitized red cells (check cells) to all negative anti-human globulin
(AHG) tests ensures that:the test was interpreted correctly - answer>>AHG reagent was added
to each test
in which of the following clinical situations will the direct antiglobulin test be positive? -
answer>>hemolytic disease of the fetus and newborn
in which of the following is the indirect antiglobulin test utilized? - answer>>antibody detection
(screening) test
polyspecific AHG reagents - answer>>anti-IgG and anti-C3d
a false negative direct antiglobulin test can be the result of: - answer>>insufficient saline
washing of red cells
a false positive indirect antiglobulin test can be the result of: - answer>>overcentrifugation
a negative result using solid phase adherence assays will demonstrate indicator red cells as: -
answer>>a RBC pellet in the bottom of the well
a 4+ positive reactions using gel technology will appear as RBCs: - answer>>in a layer at the top
of the gel media
,one of the advantages of performing antibody screening (detection) studies using gel technology
is: - answer>>saline washing is not required
monoclonal blood banking reagents have which of the following as a disadvantage? -
answer>>overspecificity
the direct antiglobulin test in a suspected case of warm autoimmune hemolytic anemia is
positive. Which of the following monospecific reagents would be used in further direct
antiglobulin testing? - answer>>anti-C3d
low ionic strength saline (LISS) acts as an enhancement medium and facilitates antibody uptake
by: - answer>>reducing zeta potential
the membrane attack complex (MAC) formed during the activation of complement via the
classical pathway consists of: - answer>>C5b through C9
in ABO hemolytic transfusion reactions, complement is activated via which of the following
pathways? - answer>>classical
IgG-sensitized red cells that activate complement may not complete complement activation to
cell lysis. In addition to IgG on the surface of these red cells, what complement component is
present? - answer>>C3b
In a normal adult, the spleen acts as a site for: - answer>>removal of aging red blood cells
which of the following cells transport O2 and CO2? - answer>>erythrocytes
which of the following cells is the largest cell in the bone marrow? - answer>>megakaryocyte
in patients who present with bleeding due to a low platelet count, the most common type of
bleeding is: - answer>>mucosal bleeding
,Transfusion of which of the following is needed to help correct hypofibrinogenemia due to DIC?
- answer>>cryoprecipitated AHF
a blood component used in the treatment of hemophilia A is: - answer>>factor VIII
a unit of Fresh Frozen Plasma was inadvertently thawed and then immediately refrigerated at 4
oC on Monday morning. On Tuesday evening this unit may still be transfused as a replacement
for: - answer>>Factor IX
a newborn demonstrates petechiae, ecchymosis and mucosal bleeding. the preferred blood
component for this infant would be: - answer>>Platelets
which of the following would be the best source of platelets for transfusion in the case of
alloimmune neonatal thrombocytopenia? - answer>>mother
fetal neonatal alloimmune thrombocytopenia (FNAIT) is caused by which of the following
maternal antibodies? - answer>>IgG alloantibodies against HPA antigens
an obstetrical patient has had 3 previous pregnancies. Her first baby was healthy, the second
was jaundiced at birth and required an exchange transfusion, while the third was stillborn.
Which of the following is the most likely cause? - answer>>Rh incompatibility
ABO-hemolytic disease of the newborn: - answer>>is usually seen only in the newborn of group
O mothers
which of the following antigens is MOST likely to be involved in hemolytic disease of the fetus
and newborn? - answer>>Kell
ABO hemolytic disease of the fetus and newborn (HDFN) differs from Rh HDFN in that: -
answer>>Rh HDFN is clinically more severe than ABO HDFN
, a group A Rh positive infant of a group O, Rh positive mother has a weakly positive DAT and
moderately elevated bilirubin 12 hours after birth. the most likely cause is: - answer>>ABO
incompatability
in suspected cases of hemolytic disease of the fetus and newborn, what significant information
can be obtained from the baby's blood smear? - answer>>determination of the presence of
spherocytes
in treating hyperbilirubinemia in hemolytic disease of teh fetus and newborn (HDFN), light
phototherapy is used to: - answer>>breakdown unconjugated bilirubin to a nontoxic isomer
the purpose of performing antibody titers on serum from an immunized pregnant woman is to: -
answer>>identify candidates requiring additional fetal monitoring
which unit should be selected for exchange transfusion if the newborn is group A, Rh-positive
and the motehr is group A, Rh-positive with anti-c? - answer>>CDe/CDe
a mother is group A, with anti-D in her serum, what would be the preferred blood product if an
intrauterine transfusion is indicated? - answer>>Rh-negative RBC, Irradiated, CMV safe, HgbS-
negative
laboratory studies of maternal and cord blood yield the following results:
maternal blood= O,
Rh negative;
anti-E in serum
cord blood = B,
Rh positive, DAT=2+, anti-E in eluate - answer>>O, Rh-positive, E-
a blood specimen from a pregnant woman is found to be group B, Rh-negative and the serum
contains anti-D with a titer of 512. What would be the most appropriate type of blood to have
available for a possible exchange transfusion for her infant? - answer>>O, Rh-negative
