ISA Certified Arboriculture Certification Comprehensive
EXAM 2026-2027 LATEST UPDATED VERSION 400
QUESTIONS AND ANSWERS JUST RELEASED
What type of knot is most effective to tie equipment to a climber's line?
(A) Midline clove hitch
(B) Girth hitch
(C) Figure-8
(D) Timber hitch - answer>>The midline clove hitch is recommended for use in pulling
equipment on climbing lines.
The answer is (A)
What is not one of the five classifications of organs for plants?
(A) Leaves
(B) Stems
(C) Buds
(D) Fruit - answer>>The five organs of a plant are:
• Leaves
• Roots
• Stems
• Fruit
• Flowers
The answer is (C)
What species of trees do not have secondary growth?
,(A) Oak
(B) Palm
(C) Pine
(D) Flowering - answer>>Palms lack secondary growth.
The answer is (B)
What type of reproduction creates a genetically identical new plant?
(A) Insect pollination
(B) Seeds
(C) Cuttings
(D) Wind pollination - answer>>Trees can reproduce in a variety of ways. Cutting is a process of
taking a stem of a tree and planting it to grow on its own. Eventually, the stem will develop a
root system and become an independent tree. This process is also known as cloning and
produces a genetically identical tree.
The answer is (C)
What component of the xylem is only present in hardwood trees?
(A) Tracheids
(B) Vessels
(C) Fibers
(D) Parenchyma cells - answer>>The xylem is tissue both living and nonliving. It is composed of
tracheids, fibers, and parenchyma cells. Hardwoods also include vessels which are long tubes
used for water conducting elements.
The answer is (A)
,Growth rings are the product of which of the following?
(A) Seasonal production of xylem
(B) Annual production of cambium
(C) Annual production of epidermis
(D) Seasonal production of cortex - answer>>The vascular cambium ring is active during the
growing season and produces a yearly ring of xylem which is also called the growth rings.
The answer is (A)
The waxy outer layer of the leaf is called which of the following?
(A) Cuticle
(B) Stomata
(C) Epidermis
(D) palisade - answer>>Leaves have a waxy outer layer called the cuticle which works to hold in
moisture and keep the leaf from drying out.
The answer is (A)
What is not one of the main regions of the root structure?
(A) Differentiation
(B) Absorption
(C) Elongation
(D) Meristemic - answer>>Roots are divided into zones based on function as they grow. This
includes the differentiation region, elongation region, and meristematic zone.
, The answer is (B)
Photosynthates are responsible for the sufficient production of all of the following except:
(A) Amino acids
(B) Oxygen
(C) Fat
(D) Proteins - answer>>Photosynthates are the carbohydrates produced as a result of
photosynthesis. They are used as the foundation for the production of many of the elements the
tree needs to survive such as amino acids, fats, proteins, starches, and others. Oxygen is a
byproduct of the photosynthesis process and is not produced by photosynthates.
The answer is (B)
A tree that alters its growth direction due to the effects of gravity is which of the following?
(A) Auxins
(B) Cytokinin
(C) Phototropism
(D) Geotropism - answer>>Trees will adapt to the forces imposed on them in their environment.
This includes changing the direction of growth. This can be generally classified as a tree's
tropism. A geotropism is a tree that has a growth pattern which is a response to the effects of
gravity.
The answer is (D)
According to the Shigo compartmentalization process, Wall 1 performs which of the following
functions?
(A) Plugging of xylem vessels
(B) Depositing of chemicals in the interior cells
EXAM 2026-2027 LATEST UPDATED VERSION 400
QUESTIONS AND ANSWERS JUST RELEASED
What type of knot is most effective to tie equipment to a climber's line?
(A) Midline clove hitch
(B) Girth hitch
(C) Figure-8
(D) Timber hitch - answer>>The midline clove hitch is recommended for use in pulling
equipment on climbing lines.
The answer is (A)
What is not one of the five classifications of organs for plants?
(A) Leaves
(B) Stems
(C) Buds
(D) Fruit - answer>>The five organs of a plant are:
• Leaves
• Roots
• Stems
• Fruit
• Flowers
The answer is (C)
What species of trees do not have secondary growth?
,(A) Oak
(B) Palm
(C) Pine
(D) Flowering - answer>>Palms lack secondary growth.
The answer is (B)
What type of reproduction creates a genetically identical new plant?
(A) Insect pollination
(B) Seeds
(C) Cuttings
(D) Wind pollination - answer>>Trees can reproduce in a variety of ways. Cutting is a process of
taking a stem of a tree and planting it to grow on its own. Eventually, the stem will develop a
root system and become an independent tree. This process is also known as cloning and
produces a genetically identical tree.
The answer is (C)
What component of the xylem is only present in hardwood trees?
(A) Tracheids
(B) Vessels
(C) Fibers
(D) Parenchyma cells - answer>>The xylem is tissue both living and nonliving. It is composed of
tracheids, fibers, and parenchyma cells. Hardwoods also include vessels which are long tubes
used for water conducting elements.
The answer is (A)
,Growth rings are the product of which of the following?
(A) Seasonal production of xylem
(B) Annual production of cambium
(C) Annual production of epidermis
(D) Seasonal production of cortex - answer>>The vascular cambium ring is active during the
growing season and produces a yearly ring of xylem which is also called the growth rings.
The answer is (A)
The waxy outer layer of the leaf is called which of the following?
(A) Cuticle
(B) Stomata
(C) Epidermis
(D) palisade - answer>>Leaves have a waxy outer layer called the cuticle which works to hold in
moisture and keep the leaf from drying out.
The answer is (A)
What is not one of the main regions of the root structure?
(A) Differentiation
(B) Absorption
(C) Elongation
(D) Meristemic - answer>>Roots are divided into zones based on function as they grow. This
includes the differentiation region, elongation region, and meristematic zone.
, The answer is (B)
Photosynthates are responsible for the sufficient production of all of the following except:
(A) Amino acids
(B) Oxygen
(C) Fat
(D) Proteins - answer>>Photosynthates are the carbohydrates produced as a result of
photosynthesis. They are used as the foundation for the production of many of the elements the
tree needs to survive such as amino acids, fats, proteins, starches, and others. Oxygen is a
byproduct of the photosynthesis process and is not produced by photosynthates.
The answer is (B)
A tree that alters its growth direction due to the effects of gravity is which of the following?
(A) Auxins
(B) Cytokinin
(C) Phototropism
(D) Geotropism - answer>>Trees will adapt to the forces imposed on them in their environment.
This includes changing the direction of growth. This can be generally classified as a tree's
tropism. A geotropism is a tree that has a growth pattern which is a response to the effects of
gravity.
The answer is (D)
According to the Shigo compartmentalization process, Wall 1 performs which of the following
functions?
(A) Plugging of xylem vessels
(B) Depositing of chemicals in the interior cells
