MCB 317 Question 2 and 3 Exam Questions with
Verified Correct Answers
quiz 2: questions 1-12, 15-17, 19, 25
quiz 3: questions 15, 20-25
look at google doc
Match the type of polymorphism with the letter of its corresponding mutational cause.
A - Unequal crossing over between homologous chromosomes
B - DNA polymerase pauses and misaligns the newly replicated strand when it reanneals
D - Transition mutation due to chemical mutagen
B: Microsatellite
D: SNPs
A: Minisatellite
What is the frequency for any individual person to have a SNP base-pair variant that is
different from the general population "consensus" wild-type chromosome?
Once every 1000 base pairs
True or false?
The length of the repeat unit of a microsatellite is longer than the length of the repeat unit of
the minisatellite
False
In the primer extension technique of SNP identification described in lecture, what method
was used to detect the reaction product?
Mass spec
True or false?
Most SNPs are bi-alleleic.
Individual SNPs are highly polymorphic.
, True
False
In the version of DNA sequencing in which four separate reactions are carried out and the
products of each reaction are made radioactive, which component of the initial reaction
mixture is radioactive?
dATP
As discussed in lecture, microarrays can be used to detect which of the following types of
polymorphic loci?
Microsatellites
SNPs
No
Yes
SNPs
1 of every 30bp in the human genome displays this type of variation in the human population as
a whole.
Polymorphism derived from a single mutation event.
Is responsible for the most common form of sickle cell anemia.
minisatellites
Polymorphism detected by DNA fingerprinting
True or false?
Most SNPs have resulted from a mutation that once in the history of the human population.
True
Which type of polymorphism is studied in DNA fingerprinting?
Minisatellite
True or false?
In dideoxysequencing, individual bands are visualize as a result of labeling of the primer
False
Verified Correct Answers
quiz 2: questions 1-12, 15-17, 19, 25
quiz 3: questions 15, 20-25
look at google doc
Match the type of polymorphism with the letter of its corresponding mutational cause.
A - Unequal crossing over between homologous chromosomes
B - DNA polymerase pauses and misaligns the newly replicated strand when it reanneals
D - Transition mutation due to chemical mutagen
B: Microsatellite
D: SNPs
A: Minisatellite
What is the frequency for any individual person to have a SNP base-pair variant that is
different from the general population "consensus" wild-type chromosome?
Once every 1000 base pairs
True or false?
The length of the repeat unit of a microsatellite is longer than the length of the repeat unit of
the minisatellite
False
In the primer extension technique of SNP identification described in lecture, what method
was used to detect the reaction product?
Mass spec
True or false?
Most SNPs are bi-alleleic.
Individual SNPs are highly polymorphic.
, True
False
In the version of DNA sequencing in which four separate reactions are carried out and the
products of each reaction are made radioactive, which component of the initial reaction
mixture is radioactive?
dATP
As discussed in lecture, microarrays can be used to detect which of the following types of
polymorphic loci?
Microsatellites
SNPs
No
Yes
SNPs
1 of every 30bp in the human genome displays this type of variation in the human population as
a whole.
Polymorphism derived from a single mutation event.
Is responsible for the most common form of sickle cell anemia.
minisatellites
Polymorphism detected by DNA fingerprinting
True or false?
Most SNPs have resulted from a mutation that once in the history of the human population.
True
Which type of polymorphism is studied in DNA fingerprinting?
Minisatellite
True or false?
In dideoxysequencing, individual bands are visualize as a result of labeling of the primer
False
