MCB 317 EXAM 3 Question 9-11 with Verified
Correct Answers
Answer the next two questions based on the following information. You are studying two
genes, gene 1 and gene 2. Gene 1 is normally off but is turned on in the presence of
progesterone. Gene 2 is constitutively expressed. You "swap" the promoter regions for these
two genes so that the promoter from gene 1 replaces the promoter from gene 2 and visa
versa.
What pattern of expression do you expect to see for the hybrid version of the gene containing
the gene 1 coding region?
What pattern of expression do you expect to see for the hybrid version of the gene containing
the gene 2 coding region?
1. Normally off but is turned on in the presence of progesterone
2. Constitutively expressed
The function for both stay the same because we're just changing the promoter, which is
essentially just the "on/off" switch. Changing this wouldn't affect the function of each gene.
The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII:
Incorrect are homologous to some RNA pol II consensus elements.
Correct: are found within the tRNA and 5S RNA coding regions.
Incorrect are found upstream of the tRNA and 5S RNA coding regions.
Incorrect are homologous to some RNA pol I consensus elements.
Incorrect are homologous to some RNA pol I and pol II consensus elements.
What is the general name for the type of DNA elements that allow eukaryotic genes to be
turned on under certain specific conditions, such as in the presence of glucocorticoid
hormones?
response element
True or false? As discussed in lecture, virtually any enhancer can work in combination with
virtually any promoter.
, True
Which eukaryotic control region typically mediates regulation of transcription?
A. Promoter
B. Enhancer
Enhancer
True or false? As discussed in lecture, linker scanner analysis defines the borders of a control
region.
False, it is deletion analysis
Transformation and transfection involve putting DNA molecules constructed in the laboratory
into cells. What is the difference between transformation and transfection?
In transformation the DNA is stably maintained and genes on transformed DNA can be
expressed over long periods of time; whereas,in transfection the DNA is not stably maintained
and genes on transfected DNA are only transiently expressed.
Based on our discussion in lecture up to 4.4.09, which DNA control elements are required for
activation of transcription by eukaryotic RNAPII in vitro? Select 'Yes' or 'No' for each.
Promoter
Silencer
Enhancer
Yes: Promoter
No: Silencer
No: Enhancer
Based on our discussion in lecture up to 4.4.09, which DNA control elements are required for
activation of transcription by eukaryotic RNAPII in vivo? Select 'Yes' or 'No' for each.
Promoter
Enhancer
Silencer
Yes: Promoter
Yes: Enhancer
No: Silencer
The promoter region for genes transcribed by which RNAP are contained entirely within the
transcribed region?
RNAPIII
Correct Answers
Answer the next two questions based on the following information. You are studying two
genes, gene 1 and gene 2. Gene 1 is normally off but is turned on in the presence of
progesterone. Gene 2 is constitutively expressed. You "swap" the promoter regions for these
two genes so that the promoter from gene 1 replaces the promoter from gene 2 and visa
versa.
What pattern of expression do you expect to see for the hybrid version of the gene containing
the gene 1 coding region?
What pattern of expression do you expect to see for the hybrid version of the gene containing
the gene 2 coding region?
1. Normally off but is turned on in the presence of progesterone
2. Constitutively expressed
The function for both stay the same because we're just changing the promoter, which is
essentially just the "on/off" switch. Changing this wouldn't affect the function of each gene.
The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII:
Incorrect are homologous to some RNA pol II consensus elements.
Correct: are found within the tRNA and 5S RNA coding regions.
Incorrect are found upstream of the tRNA and 5S RNA coding regions.
Incorrect are homologous to some RNA pol I consensus elements.
Incorrect are homologous to some RNA pol I and pol II consensus elements.
What is the general name for the type of DNA elements that allow eukaryotic genes to be
turned on under certain specific conditions, such as in the presence of glucocorticoid
hormones?
response element
True or false? As discussed in lecture, virtually any enhancer can work in combination with
virtually any promoter.
, True
Which eukaryotic control region typically mediates regulation of transcription?
A. Promoter
B. Enhancer
Enhancer
True or false? As discussed in lecture, linker scanner analysis defines the borders of a control
region.
False, it is deletion analysis
Transformation and transfection involve putting DNA molecules constructed in the laboratory
into cells. What is the difference between transformation and transfection?
In transformation the DNA is stably maintained and genes on transformed DNA can be
expressed over long periods of time; whereas,in transfection the DNA is not stably maintained
and genes on transfected DNA are only transiently expressed.
Based on our discussion in lecture up to 4.4.09, which DNA control elements are required for
activation of transcription by eukaryotic RNAPII in vitro? Select 'Yes' or 'No' for each.
Promoter
Silencer
Enhancer
Yes: Promoter
No: Silencer
No: Enhancer
Based on our discussion in lecture up to 4.4.09, which DNA control elements are required for
activation of transcription by eukaryotic RNAPII in vivo? Select 'Yes' or 'No' for each.
Promoter
Enhancer
Silencer
Yes: Promoter
Yes: Enhancer
No: Silencer
The promoter region for genes transcribed by which RNAP are contained entirely within the
transcribed region?
RNAPIII
