BLOOD BANK EXAM 2 UPDATED EXAM SCRIPT
WITH COMPLETE ANSWERS
◉ H antigen.
Answer: ALL RBCs, regardless of ABO type, express this antigen
◉ Glycosyltransferases.
Answer: ABO, Hh, and Se Genes encode for _________________, which
causes transfer of monosaccharide molecule onto a precursor
substance on the RBC
◉ H gene.
Answer: Codes for fucosyltransferase (FUT1) that adds fucose to
terminal galactose of type 2 precursor chains to make H antigen
Must be inherited to form ABO antigens on RBCS
◉ A gene.
Answer: Codes for A-trasnferase that adds N-acetylgalactosamine
(GalNac) to subterminal galactose of the preformed H antigen to
form A antigen
◉ B gene.
,Answer: Codes for B-transferase that adds D galactose (Gal) to
subterminal galactose of H antigen to form B antigen
◉ Type 1 precursor chains.
Answer: Found in secretions (saliva, breast milk etc)
Gal-beta (1->3)- GlcNac
◉ Type 2 precursor chains.
Answer: Found on RBC surface
Gal-beta (1->4)- GlacNac
◉ Chromosome 19.
Answer: FUT 1 (H) and FUT 2 (Se) genes are closely linked and
located on what chromosome?
◉ Se gene (secretor gene).
Answer: Gene that must be inherited to form ABO antigens in
secretions
This gene encodes a fucosyl transferase that adds fucose to type 1
precursor chains, forming H antigen
◉ Chromosome 9.
Answer: ABO gene is located on what chromosome?
,◉ Codominant.
Answer: A and B antigens demonstrate ______________ expression
◉ O = 45, A = 40, B = 11, AB = 4.
Answer: Percentages of blood type in Caucasian population (most
common since most blood donors are white)
◉ Weaker.
Answer: Reactions of newborn RBSd with ABO reagent antisera are
_________ than adults
◉ 80;20 (sese genotype).
Answer: ______ % of the pop are secretors (ABH antigens in body
secretions), ______% are nonsecretors (no ABH soluble antigens in
secretions)
◉ Exon 6 and 7.
Answer: These exons of ABO genes encode for catalytic domain of
ABO glyocsyltransferases. Amino acid substitutions are responsible
for less efficient transfer of H substance, which results in weak
serologic reaction in ABO subgroups
◉ A1; A2.
, Answer: ______ is the most common A subtype (80%) and 20% are
_______
◉ a1.
Answer: In type a2 people, they will have anti-_________, which in
clinically significant. (Type A antigen in forward, will also have type
A antibodies against this in reverse reaction)
◉ Dolichos biflorus (A1 lectin).
Answer: The lectin that agglutinates A1 cells but not A2 cells,, used
to differentiate between A subtypes
◉ Bandeiraea simplicifolia.
Answer: Lectin that agglutinates B cells
◉ Ulex europaeus.
Answer: lectin that agglutinates O cells (H specificity) and other ABO
blood groups depending on amount of H antigen available
◉ More;less.
Answer: The ______ A antigen is formed, the ______ the H antigen will
be expressed on the RBC
WITH COMPLETE ANSWERS
◉ H antigen.
Answer: ALL RBCs, regardless of ABO type, express this antigen
◉ Glycosyltransferases.
Answer: ABO, Hh, and Se Genes encode for _________________, which
causes transfer of monosaccharide molecule onto a precursor
substance on the RBC
◉ H gene.
Answer: Codes for fucosyltransferase (FUT1) that adds fucose to
terminal galactose of type 2 precursor chains to make H antigen
Must be inherited to form ABO antigens on RBCS
◉ A gene.
Answer: Codes for A-trasnferase that adds N-acetylgalactosamine
(GalNac) to subterminal galactose of the preformed H antigen to
form A antigen
◉ B gene.
,Answer: Codes for B-transferase that adds D galactose (Gal) to
subterminal galactose of H antigen to form B antigen
◉ Type 1 precursor chains.
Answer: Found in secretions (saliva, breast milk etc)
Gal-beta (1->3)- GlcNac
◉ Type 2 precursor chains.
Answer: Found on RBC surface
Gal-beta (1->4)- GlacNac
◉ Chromosome 19.
Answer: FUT 1 (H) and FUT 2 (Se) genes are closely linked and
located on what chromosome?
◉ Se gene (secretor gene).
Answer: Gene that must be inherited to form ABO antigens in
secretions
This gene encodes a fucosyl transferase that adds fucose to type 1
precursor chains, forming H antigen
◉ Chromosome 9.
Answer: ABO gene is located on what chromosome?
,◉ Codominant.
Answer: A and B antigens demonstrate ______________ expression
◉ O = 45, A = 40, B = 11, AB = 4.
Answer: Percentages of blood type in Caucasian population (most
common since most blood donors are white)
◉ Weaker.
Answer: Reactions of newborn RBSd with ABO reagent antisera are
_________ than adults
◉ 80;20 (sese genotype).
Answer: ______ % of the pop are secretors (ABH antigens in body
secretions), ______% are nonsecretors (no ABH soluble antigens in
secretions)
◉ Exon 6 and 7.
Answer: These exons of ABO genes encode for catalytic domain of
ABO glyocsyltransferases. Amino acid substitutions are responsible
for less efficient transfer of H substance, which results in weak
serologic reaction in ABO subgroups
◉ A1; A2.
, Answer: ______ is the most common A subtype (80%) and 20% are
_______
◉ a1.
Answer: In type a2 people, they will have anti-_________, which in
clinically significant. (Type A antigen in forward, will also have type
A antibodies against this in reverse reaction)
◉ Dolichos biflorus (A1 lectin).
Answer: The lectin that agglutinates A1 cells but not A2 cells,, used
to differentiate between A subtypes
◉ Bandeiraea simplicifolia.
Answer: Lectin that agglutinates B cells
◉ Ulex europaeus.
Answer: lectin that agglutinates O cells (H specificity) and other ABO
blood groups depending on amount of H antigen available
◉ More;less.
Answer: The ______ A antigen is formed, the ______ the H antigen will
be expressed on the RBC
