BLOOD BANK EXAM 1 ACTUAL QUESTIONS AND CORRECT ANSWERS

BLOOD BANK EXAM 1 ACTUAL QUESTIONS
AND CORRECT ANSWERS

◉ Serum have what in terms of Ag/Ab binding.
Answer: Ab's


◉ Degree of exposure (DOSE).
Answer: amount of Ag present to invoke an immune response


◉ idiotype.
Answer: specific type of immunoglobulin


◉ isotype.
Answer: broad class of immunoglobulin like G,A,M,D,E


◉ alloantibody.
Answer: makes Ab to foreign Ag


◉ autoantibody.
Answer: makes Ab against self Ag's

,◉ hemagglutination.
Answer: visible clumping of RBCs due to Ag binding to Ab


◉ stages of agglutination (2).
Answer: 1. coating/sensitization (helps IgG)
2. lattice formation (allows visualization)


◉ Ag/Ab binding has to be what pH to occur.
Answer: 6.5-7.5


◉ isotonic reagent that cells have to be SUSPENDED IN.
Answer: physiological saline (0.85%)


◉ what net charge do RBC have (+ and -).
Answer: negative net charge


◉ zeta potential.
Answer: difference between electrostatic potential between RBC's
surface and ionic cloud


◉ prozone.
Answer: excess Ab

, ◉ postzone.
Answer: excess Ag


◉ serum:cell ratio.
Answer: 2:1 (2 drops of serum, 1 drop of cell suspension)


◉ reagent LISS stands for.
Answer: lower ionic strength solution


◉ zygosity.
Answer: dosage of an allele


◉ stronger agglutination when homozygous or heterozygous?.
Answer: homozygous


◉ amorphic.
Answer: silent allele


◉ most blood groups are (dominant, recessive, or codominant).
Answer: codominant