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“Lab 2 Culturing & Aseptic Technique BIO250L”
Student Name: Yamira Cobb
Access Code (located on the underside of the lid of your lab kit): AC-9JM12U
“Pre-Lab Questions”
1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one
experimental way you can test your practices to confirm that you are using proper aseptic
technique?
Utilizing a sterile flared circle to move material, a sterile pipet to move fluid,
and confining an unadulterated culture are completely ensured approaches
to check you're following appropriate aseptic convention.
2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
a. Using bleaching solvents
b. Using autoclave
c. Using a flame
3. For each inoculation tool, give one scenario in which use of that tool would be appropriate.
When collecting microbiological samples from surfaces, sterile cotton
swabs are typically employed. When transferring microorganisms from one
plate to another, inoculation loops would be employed. When collecting
germs from a specific area, inoculation needles are employed. To transmit
an even bacterial lawn, sterile plate spreaders are utilized.
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“Lab 2 Culturing & Aseptic Technique BIO250L”
4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some
steps you could take to extend the lifespan of a microbial culture?
They have restricted region to develop and nourishment since they are not
presented to oxygen. To make the microbial culture last more. There are a
few stages one can take. Beginning with a decent medium arranged to give
ideal help. Having the right labware for blending media, arrangement is
significant and realizing how to utilize gear for gauging, and estimating. In
the creation of culture media, sterility is vital; it is the most common region
where things turn out badly. When cleaning society media, it's basic to
adhere to the guidelines precisely so that key microbial supplements and
different parts aren't obliterated. Poisons don't frame because of high
warming. In the formation of culture media, sterility is significant; it's the
most common locale for defilement.
5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a
continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli,
which is used to make human insulin.
The microscopic organisms E. coli is critical in present day biotechnology.
Researchers use it to store DNA groupings from different animals, make
proteins, and assess the capacity of proteins. E. coli can create in the
presence or nonattendance of oxygen. Investigations using E. coli can be
done because of the fast expansion of the microbes. quick and without any
problem.
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https://www.stuvia.com/user/allatopnotchPage
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https://www.stuvia.com/user/allatopnotchPage
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1 of 9https://www.stuvia.com/user/allatopnotchhttps://www.stuvia.com/user/allatopno
“Lab 2 Culturing & Aseptic Technique BIO250L”
Student Name: Yamira Cobb
Access Code (located on the underside of the lid of your lab kit): AC-9JM12U
“Pre-Lab Questions”
1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one
experimental way you can test your practices to confirm that you are using proper aseptic
technique?
Utilizing a sterile flared circle to move material, a sterile pipet to move fluid,
and confining an unadulterated culture are completely ensured approaches
to check you're following appropriate aseptic convention.
2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment?
a. Using bleaching solvents
b. Using autoclave
c. Using a flame
3. For each inoculation tool, give one scenario in which use of that tool would be appropriate.
When collecting microbiological samples from surfaces, sterile cotton
swabs are typically employed. When transferring microorganisms from one
plate to another, inoculation loops would be employed. When collecting
germs from a specific area, inoculation needles are employed. To transmit
an even bacterial lawn, sterile plate spreaders are utilized.
ps://www.stuvia.com/user/allatopnotchPage
https://www.stuvia.com/user/allatopnotchPage
1 of 9https://www.stuvia.com/user/allatopnotchhttps://www.stuvia.com/user/allatopnotch
1 of 9https://www.stuvia.com/user/allatopnotchhttps://www.stuvia.com/user/allatopno
,ps://www.stuvia.com/user/allatopnotchPage
https://www.stuvia.com/user/allatopnotchPage
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2 of 9https://www.stuvia.com/user/allatopnotchhttps://www.stuvia.com/user/allatopno
“Lab 2 Culturing & Aseptic Technique BIO250L”
4. Why don’t microorganisms in cultures exhibit constant exponential growth? What are some
steps you could take to extend the lifespan of a microbial culture?
They have restricted region to develop and nourishment since they are not
presented to oxygen. To make the microbial culture last more. There are a
few stages one can take. Beginning with a decent medium arranged to give
ideal help. Having the right labware for blending media, arrangement is
significant and realizing how to utilize gear for gauging, and estimating. In
the creation of culture media, sterility is vital; it is the most common region
where things turn out badly. When cleaning society media, it's basic to
adhere to the guidelines precisely so that key microbial supplements and
different parts aren't obliterated. Poisons don't frame because of high
warming. In the formation of culture media, sterility is significant; it's the
most common locale for defilement.
5. Using a textbook or a reputable online source, describe how lab cultures are maintained in a
continual pattern of growth. Focus particularly on those used in biotechnology, such as E. coli,
which is used to make human insulin.
The microscopic organisms E. coli is critical in present day biotechnology.
Researchers use it to store DNA groupings from different animals, make
proteins, and assess the capacity of proteins. E. coli can create in the
presence or nonattendance of oxygen. Investigations using E. coli can be
done because of the fast expansion of the microbes. quick and without any
problem.
ps://www.stuvia.com/user/allatopnotchPage
https://www.stuvia.com/user/allatopnotchPage
2 of 9https://www.stuvia.com/user/allatopnotchhttps://www.stuvia.com/user/allatopnotch
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