MICRO 3060 LAB FINAL QUESTIONS WITH VERIFIED ANSWERS
Correct 66
Incorrect 00
1 of 66
Term
Define endemic
Give this one a try later!
infection that is completely
infection of a specific area
eradicated from the earth
infection of the entire global infection that appears suddenly and
population disappears quickly
Don't know?
2 of 66
,Term
explain how we used serological typing to identify S. aureus
Give this one a try later!
-latex reagent in center of card
-evaluates the quality of milk
with antibody
-if bacteria are present, they cause
-place a colony of S> aureus in
exhaustion of dissolved O2, indicating
latex drop
a lighter color
-observe
-resistant: very small zone of
-swab the nose and inoculate one
inhibition
quadrant of a blood agar
-intermediate: medium zone of
-streak for isolation in each quadrant
inhibition
-repeat on MSA plate
-susceptible: large zone of inhibition
Don't know?
3 of 66
Term
Know the basic steps to isolate DNA from bacterial cells.
Give this one a try later!
, -sodium dodecyl sulfate detergent releases DNA from bacterial cells by
breaking lipids in cell membranes
-proteinase K and heat degrade proteins in the mixture
-ice cold isopropyl alcohol precipitates DNA
-blood: complete hemolysis (opaque yellow colonies)
-MSA: turns the red agar yellow, salt tolerant
-CHROMagar: small pink colonies
-dark center colonies: lactose fermenters
-pale colonies: cannot ferment lactose
-E.coli: small, dark, green sheen
-E. aerogenes: larger, dark center with light border, fish eyed
-gradient plating method is used to isolate antibiotic resistant mutants
-3 plates used in replica plating method are nutrient agar plate, streptomycin plate,
and a spread plate from the previouc week
Don't know?
4 of 66
Definition
-dark center colonies: lactose fermenters
-pale colonies: cannot ferment lactose
-E.coli: small, dark, green sheen
-E. aerogenes: larger, dark center with light border, fish eyed
Give this one a try later!
, how did we test for the presence of EMB agar results meaning for
ammonification in the soil? confirmed test
Describe how we isolated S. aureus Know the basic steps to isolate DNA
from our noses from bacterial cells.
Don't know?
5 of 66
Term
Explain the Filter Paper Disk Method of evaluating the relative
effectiveness of various disinfectants and/or antiseptics.
Give this one a try later!
-inoculating 10 single strength lactose broth tubes and 5 double strength lactose
broth tubes with 1mL, 0.1mL, and 10mL of water sample
-positive tests will have a gas bubble at the top
-Most Probable Number of coliforms was calculated
-before: sweet grape smell, no bubbles present, balloon was flat, slightly acidic pH
-after: sharp, alcohol smell, bubbles present but balloon was not filled, pH dropped
-inoculate an agar pour with a loopful of organism and allow to solidify in
empty petri plate
-use sterilized forceps to pick up filter paper disk and dip it into listerine
Correct 66
Incorrect 00
1 of 66
Term
Define endemic
Give this one a try later!
infection that is completely
infection of a specific area
eradicated from the earth
infection of the entire global infection that appears suddenly and
population disappears quickly
Don't know?
2 of 66
,Term
explain how we used serological typing to identify S. aureus
Give this one a try later!
-latex reagent in center of card
-evaluates the quality of milk
with antibody
-if bacteria are present, they cause
-place a colony of S> aureus in
exhaustion of dissolved O2, indicating
latex drop
a lighter color
-observe
-resistant: very small zone of
-swab the nose and inoculate one
inhibition
quadrant of a blood agar
-intermediate: medium zone of
-streak for isolation in each quadrant
inhibition
-repeat on MSA plate
-susceptible: large zone of inhibition
Don't know?
3 of 66
Term
Know the basic steps to isolate DNA from bacterial cells.
Give this one a try later!
, -sodium dodecyl sulfate detergent releases DNA from bacterial cells by
breaking lipids in cell membranes
-proteinase K and heat degrade proteins in the mixture
-ice cold isopropyl alcohol precipitates DNA
-blood: complete hemolysis (opaque yellow colonies)
-MSA: turns the red agar yellow, salt tolerant
-CHROMagar: small pink colonies
-dark center colonies: lactose fermenters
-pale colonies: cannot ferment lactose
-E.coli: small, dark, green sheen
-E. aerogenes: larger, dark center with light border, fish eyed
-gradient plating method is used to isolate antibiotic resistant mutants
-3 plates used in replica plating method are nutrient agar plate, streptomycin plate,
and a spread plate from the previouc week
Don't know?
4 of 66
Definition
-dark center colonies: lactose fermenters
-pale colonies: cannot ferment lactose
-E.coli: small, dark, green sheen
-E. aerogenes: larger, dark center with light border, fish eyed
Give this one a try later!
, how did we test for the presence of EMB agar results meaning for
ammonification in the soil? confirmed test
Describe how we isolated S. aureus Know the basic steps to isolate DNA
from our noses from bacterial cells.
Don't know?
5 of 66
Term
Explain the Filter Paper Disk Method of evaluating the relative
effectiveness of various disinfectants and/or antiseptics.
Give this one a try later!
-inoculating 10 single strength lactose broth tubes and 5 double strength lactose
broth tubes with 1mL, 0.1mL, and 10mL of water sample
-positive tests will have a gas bubble at the top
-Most Probable Number of coliforms was calculated
-before: sweet grape smell, no bubbles present, balloon was flat, slightly acidic pH
-after: sharp, alcohol smell, bubbles present but balloon was not filled, pH dropped
-inoculate an agar pour with a loopful of organism and allow to solidify in
empty petri plate
-use sterilized forceps to pick up filter paper disk and dip it into listerine
